Rhizobium rhizogenes rolC gene promotes leaf senescence and enhances osmotic stress resistance in Arabidopsis: the positive role of abscisic acid

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Theroot oncogenic loci(rol) C oncogene has been identified as a key player during theplant-Rhizobium rhizogenesinteraction and is considered to confer resistance to plantabiotic stresses, yet the underlying mechanisms remain largely elusive. In this study,Arabidopsis plants constitutively overexpressingrolC were produced. Biometric anal-ysis showed thatrolC induced diverse phenotypic modifications, including dwarfism,increased number of stem branches (1.3 times more than WT), weak root growth,early flowering (1.6 days earlier than WT) and premature leaf senescence. In addition,senescence stimulus (exogenous ethylene: 10 or 100μLL1) and 10% (w/v) polyeth-ylene glycol 6000 (PEG) treatments demonstrated thatrolC mediated leaf abscisicacid (ABA) enhancement in Arabidopsis, and this might be involved inrolC-inducedpremature leaf senescence and resistance enhancement to osmotic stress. It is con-cluded thatrolC-induced premature leaf senescence may be involved in an abioticstress escape mechanism in Arabidopsis, which is closely related to the increase ofendogenous ABA levels. These findings provide new insights into the role ofrolCinplant-bacterial interaction and uncover the potential of biotechnological applicationassociated withR. rhizogenes/rolgenes in plant drought defense.
TidsskriftPhysiologia Plantarum
Udgave nummer1
Antal sider15
StatusUdgivet - 2024

Bibliografisk note

Funding Information:
The Chinese Scholarship Council provided scholarships to XC (PhD grant no. 202106850009) and JH (PhD grant no. 201906760024). HL, IGM and BF were supported by a SPRINT grant (2019/08846–2) provided by The São Paulo Research Foundation (FAPESP). The Brazilian Federal Agency for Support and Evaluation of Graduate Education (Capes) provided a scholarship to BF (PRINT/Capes grant no. 88887.469034/2019–00). IGM is a Brazilian National Council for Scientific and Technological Development (CNPq) productivity research fellow (CNPQ: 301043/2018–3).

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