Morgane Ourry

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April 2021-now: Postdoctoral researcher, Department of Plant and Environmental Sciences, University of Copenhagen, Frederiksberg (Denmark)

H2020 Excalibur project aims to enhance the knowledge on soil biodiversity dynamics and its synergistic effects with prebiotic and probiotic approaches in horticulture, using a multi-actor approach

• Carry out trials in strawberry integrated and organic fields to assess the effects of fungal inoculum on the plant growth, production and health, as well as on the soil biodiversity;
• Develop markers to detect and quantify fungal inoculum in soil to assess its persistence in the field;
• Study the molecular mechanisms driving the interaction between strawberry plants, spider mites and entomopathogenic fungi under control conditions.

 

June-September 2020: Research engineer, UMR INRAE 1349 IGEPP, Rennes (France)

Testing a biopesticide on the cabbage root fly development Delia radicum

• In vivo and in vitro experiments
• Phenotype measurements and statistical analyses

 

November 2016-December 2019: PhD student, UMR INRAE 1349 IGEPP, Le Rheu (France). Thesis manuscript

Study the interaction between oilseed rape (Brassica napus), the cabbage root fly (Delia radicum) and their associated microbial communities, in order to:

1. Assess the effect of soil microbial diversity on plant chemistry and insect life history traits (doi: 10.1111/1744-7917.12478);
2. Assess the effect of insect herbivory on root chemistry and both root and rhizosphere microbial communities (doi: 10.3389/fevo.2018.00091);
3. Determine the effect of antibiotics on insect bacterial communities (doi: 10.1093/femsec/fiaa028);
4. Evaluate vertical and horizontal bacterial transmission in D. radicum.

 

• Bibliography monitoring
• Protocol development, planning and implementation of experiments by coordinating several people
• Inoculation of microorganisms and pathogens in soil assessment of disease symptoms and insect damages
• Bacterial and fungal culture
• DNA extraction (soil, plant, insect), library preparation
• PCR amplification and DNA quantification (Nanodrop, Quantus), electrophoresis
• Bioinformatical analysis of Illumina MiSeq paired-end sequences (DADA)
• Statistical analyses and interpretation of metabolomic and metabarcoding data: uni and multivariate, differential heat trees, networks, function prediction
• Writing, restitution, synthesis and popularization of results
• Teaching at the University de Rennes 1 24 h in the Ecology course for the 1st year BS students
• Organization of a national congress event management and planning, funding collection and budget management

 

January-July 2016: Master 2 internship, UMR INRAE 1349 IGEPP, Le Rheu (France)

Assess the effects of the rhizosphere microbial diversity on the defense modulation of B. napus interacting with D. radicum

• Plant and insect phenotype measurements
• Leaf and root amino acid, carbohydrate, glucosinolate extractions

 

April-May 2015: Master 1 internship, UMR CNRS 6553 ECOBIO, Rennes (France)

Assess the effects of gradual acclimation on Drosophila melanogaster apoptosis and necrosis Diptera Drosophilidae

• Fly breeding
• Protein quantification