Parasitic plant small RNA analyses unveil parasite-specific signatures of microRNA retention, loss, and gain

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Parasitism is a successful life strategy that has evolved independently in several families of vascular plants. The genera Cuscuta and Orobanche represent examples of the two profoundly different groups of parasites: one parasitizing host shoots and the other infecting host roots. In this study, we sequenced and described the overall repertoire of small RNAs from Cuscuta campestris and Orobanche aegyptiaca. We showed that Cuscuta campestris contains a number of novel microRNAs (miRNAs) in addition to a conspicuous retention of miRNAs that are typically lacking in other Solanales, while several typically conserved miRNAs seem to have become obsolete in the parasite. One new miRNA appears to be derived from a horizontal gene transfer event. The exploratory analysis of the miRNA population (exploratory due to the absence of a full genomic sequence for reference) from the root parasitic Orobanche aegyptiaca also revealed a loss of a number of miRNAs compared to photosynthetic species from the same order. In summary, our study shows partly similar evolutionary signatures in the RNA silencing machinery in both parasites. Our data bear proof for the dynamism of this regulatory mechanism in parasitic plants.

OriginalsprogEngelsk
TidsskriftPlant Physiology
Vol/bind190
Udgave nummer2
Sider (fra-til)1242-1259
ISSN0032-0889
DOI
StatusUdgivet - 2022

Bibliografisk note

© The Author(s) 2022. Published by Oxford University Press on behalf of American Society of Plant Biologists.

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