TY - JOUR

T1 - Insights on strain 115 plant growth-promoting bacteria traits and its contribution in lead stress alleviation in pea (Pisum sativum L.) plants

AU - Chamekh, Anissa

AU - Kharbech, Oussama

AU - Fersi, Cheima

AU - Driss Limam, Rim

AU - Brandt, Kristian Koefed

AU - Djebali, Wahbi

AU - Chouari, Rakia

PY - 2023

Y1 - 2023

N2 - The present study aims to characterize the plant growth-promoting bacterial traits of Bacillus simplex (strain 115). This bacterium was inoculated in hydroponically conditions to improve pea (Pisum sativum L.) growth submitted to lead (Pb) toxicity. Root nodulation system was developed enough in 23-day-old plants attesting the interaction between the two organisms. In addition to its phosphate solubilization and siderophore production traits that reached 303.8 mu g P mL(-1) and 49.6 psu respectively, the Bacillus strain 115 exhibited Pb bio-sorption ability. Inoculation of Pb-stressed pea with strain 115 showed roots and shoots biomass recovery (+ 70% and + 61%, respectively). Similarly, water and protein contents were increased in Pb-treated plants after bacterial inoculation. In the presence of strain 115, Pb relative toxicity level decreased (- 39.3% compared to Pb stress only). Moreover, catalase and superoxide dismutase activities were upregulated in Pb-exposed plants (+ 56% and + 51%, respectively). After inoculation with strain 115, catalase and superoxide dismutase activities were restored by - 38% and - 44% respectively. Simultaneously, oxidant stress indicator (H2O2 and 4-hydroxynonenal) and osmo-regulators (proline and glycine-betaine) contents as well as lipoxygenase activity decreased significantly in Pb-treated plants after Bacillus strain's inoculation. Taken together, the results give some evidences for the plant growth-promoting capacity of strain 115 in helping alleviation of Pb stress.

AB - The present study aims to characterize the plant growth-promoting bacterial traits of Bacillus simplex (strain 115). This bacterium was inoculated in hydroponically conditions to improve pea (Pisum sativum L.) growth submitted to lead (Pb) toxicity. Root nodulation system was developed enough in 23-day-old plants attesting the interaction between the two organisms. In addition to its phosphate solubilization and siderophore production traits that reached 303.8 mu g P mL(-1) and 49.6 psu respectively, the Bacillus strain 115 exhibited Pb bio-sorption ability. Inoculation of Pb-stressed pea with strain 115 showed roots and shoots biomass recovery (+ 70% and + 61%, respectively). Similarly, water and protein contents were increased in Pb-treated plants after bacterial inoculation. In the presence of strain 115, Pb relative toxicity level decreased (- 39.3% compared to Pb stress only). Moreover, catalase and superoxide dismutase activities were upregulated in Pb-exposed plants (+ 56% and + 51%, respectively). After inoculation with strain 115, catalase and superoxide dismutase activities were restored by - 38% and - 44% respectively. Simultaneously, oxidant stress indicator (H2O2 and 4-hydroxynonenal) and osmo-regulators (proline and glycine-betaine) contents as well as lipoxygenase activity decreased significantly in Pb-treated plants after Bacillus strain's inoculation. Taken together, the results give some evidences for the plant growth-promoting capacity of strain 115 in helping alleviation of Pb stress.

KW - Antioxidants

KW - Bacillus simplex

KW - Lead

KW - Pisum sativum

KW - OXIDATIVE STRESS

KW - NITRIC-OXIDE

KW - ASSAY

KW - PHOSPHATE

KW - SEEDLINGS

KW - CADMIUM

KW - COPPER

U2 - 10.1007/s00203-022-03341-7

DO - 10.1007/s00203-022-03341-7

M3 - Journal article

C2 - 36436136

VL - 205

JO - Archives of Microbiology

JF - Archives of Microbiology

SN - 0302-8933

M1 - 1

ER -