Development and application of a suite of polysaccharide-degrading enzymes for analyzing plant cell walls
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
To facilitate analysis of plant cell wall polysaccharide structure and composition, we cloned 74 genes encoding polysaccharide-degrading enzymes from Aspergillus nidulans, Aspergillus fumigatus, and Neurospora crassa and expressed the genes as secreted proteins with C-terminal Myc and 6x His tags. Most of the recombinant enzymes were active in enzyme assays, and optima for pH and temperature were established. A subset of the enzymes was used to fragment polysaccharides from the irregular xylem 9 (irx9) mutant of Arabidopsis. The analysis revealed a decrease in the abundance of xylan in the mutant, indicating that the IRX9 gene, which encodes a putative family 43 glycosyltransferase, is required for xylan synthesis.
Originalsprog | Engelsk |
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Tidsskrift | Proceedings of the National Academy of Sciences of the United States of America |
Vol/bind | 103 |
Udgave nummer | 30 |
Sider (fra-til) | 11417-11422 |
Antal sider | 6 |
ISSN | 0027-8424 |
DOI | |
Status | Udgivet - 2006 |
Eksternt udgivet | Ja |
ID: 273641626