The Arabidopsis male-sterile mutant dde2-2 is defective in the ALLENE OXIDE SYNTHASE gene encoding one of the key enzymes of the jasmonic acid biosynthesis pathway

Research output: Contribution to journal › Journal article › Research

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The Arabidopsis male-sterile mutant dde2-2 is defective in the ALLENE OXIDE SYNTHASE gene encoding one of the key enzymes of the jasmonic acid biosynthesis pathway. / von Malek, Bernadette; van der Graaff, Eric; Schneitz, Kay; Keller, Beat.

In: Planta, Vol. 216, No. 1, 11.2002, p. 187-92.

Research output: Contribution to journal › Journal article › Research

Harvard

von Malek, B, van der Graaff, E, Schneitz, K & Keller, B 2002, 'The Arabidopsis male-sterile mutant dde2-2 is defective in the ALLENE OXIDE SYNTHASE gene encoding one of the key enzymes of the jasmonic acid biosynthesis pathway', Planta, vol. 216, no. 1, pp. 187-92. https://doi.org/10.1007/s00425-002-0906-2

APA

von Malek, B., van der Graaff, E., Schneitz, K., & Keller, B. (2002). The Arabidopsis male-sterile mutant dde2-2 is defective in the ALLENE OXIDE SYNTHASE gene encoding one of the key enzymes of the jasmonic acid biosynthesis pathway. Planta, 216(1), 187-92. https://doi.org/10.1007/s00425-002-0906-2

Vancouver

von Malek B, van der Graaff E, Schneitz K, Keller B. The Arabidopsis male-sterile mutant dde2-2 is defective in the ALLENE OXIDE SYNTHASE gene encoding one of the key enzymes of the jasmonic acid biosynthesis pathway. Planta. 2002 Nov;216(1):187-92. https://doi.org/10.1007/s00425-002-0906-2

Author

von Malek, Bernadette ; van der Graaff, Eric ; Schneitz, Kay ; Keller, Beat. / The Arabidopsis male-sterile mutant dde2-2 is defective in the ALLENE OXIDE SYNTHASE gene encoding one of the key enzymes of the jasmonic acid biosynthesis pathway. In: Planta. 2002 ; Vol. 216, No. 1. pp. 187-92.

Bibtex

@article{c3ae4e9f641a4cf98b7283dffc810d28,
title = "The Arabidopsis male-sterile mutant dde2-2 is defective in the ALLENE OXIDE SYNTHASE gene encoding one of the key enzymes of the jasmonic acid biosynthesis pathway",
abstract = "The Arabidopsis thaliana (L.) Heynh. mutant delayed-dehiscence2-2 (dde2-2) was identified in an En1/Spm1 transposon-induced mutant population screened for plants showing defects in fertility. The dde2-2 mutant allele is defective in the anther dehiscence process and filament elongation and thus exhibits a male-sterile phenotype. The dde2-2 phenotype can be rescued by application of methyl jasmonate, indicating that the mutant is affected in jasmonic acid biosynthesis. The combination of genetic mapping and a candidate-gene approach identified a frameshift mutation in the ALLENE OXIDE SYNTHASE (AOS) gene, encoding one of the key enzymes of jasmonic acid biosynthesis. Expression analysis and genetic complementation of the dde2-2 phenotype by overexpression of the AOS coding sequence confirmed that the male-sterile phenotype is indeed caused by the mutation in the AOS gene.",
keywords = "Acetates, Amino Acid Sequence, Arabidopsis, Chromosome Mapping, Chromosomes, Plant, Cyclopentanes, Fertility, Genetic Complementation Test, Intramolecular Oxidoreductases, Molecular Sequence Data, Mutation, Oxylipins, Sequence Homology, Amino Acid",
author = "{von Malek}, Bernadette and {van der Graaff}, Eric and Kay Schneitz and Beat Keller",
year = "2002",
month = nov,
doi = "10.1007/s00425-002-0906-2",
language = "English",
volume = "216",
pages = "187--92",
journal = "Planta",
issn = "0032-0935",
publisher = "Springer",
number = "1",

}

RIS

TY - JOUR

T1 - The Arabidopsis male-sterile mutant dde2-2 is defective in the ALLENE OXIDE SYNTHASE gene encoding one of the key enzymes of the jasmonic acid biosynthesis pathway

AU - von Malek, Bernadette

AU - van der Graaff, Eric

AU - Schneitz, Kay

AU - Keller, Beat

PY - 2002/11

Y1 - 2002/11

N2 - The Arabidopsis thaliana (L.) Heynh. mutant delayed-dehiscence2-2 (dde2-2) was identified in an En1/Spm1 transposon-induced mutant population screened for plants showing defects in fertility. The dde2-2 mutant allele is defective in the anther dehiscence process and filament elongation and thus exhibits a male-sterile phenotype. The dde2-2 phenotype can be rescued by application of methyl jasmonate, indicating that the mutant is affected in jasmonic acid biosynthesis. The combination of genetic mapping and a candidate-gene approach identified a frameshift mutation in the ALLENE OXIDE SYNTHASE (AOS) gene, encoding one of the key enzymes of jasmonic acid biosynthesis. Expression analysis and genetic complementation of the dde2-2 phenotype by overexpression of the AOS coding sequence confirmed that the male-sterile phenotype is indeed caused by the mutation in the AOS gene.

AB - The Arabidopsis thaliana (L.) Heynh. mutant delayed-dehiscence2-2 (dde2-2) was identified in an En1/Spm1 transposon-induced mutant population screened for plants showing defects in fertility. The dde2-2 mutant allele is defective in the anther dehiscence process and filament elongation and thus exhibits a male-sterile phenotype. The dde2-2 phenotype can be rescued by application of methyl jasmonate, indicating that the mutant is affected in jasmonic acid biosynthesis. The combination of genetic mapping and a candidate-gene approach identified a frameshift mutation in the ALLENE OXIDE SYNTHASE (AOS) gene, encoding one of the key enzymes of jasmonic acid biosynthesis. Expression analysis and genetic complementation of the dde2-2 phenotype by overexpression of the AOS coding sequence confirmed that the male-sterile phenotype is indeed caused by the mutation in the AOS gene.

KW - Acetates

KW - Amino Acid Sequence

KW - Arabidopsis

KW - Chromosome Mapping

KW - Chromosomes, Plant

KW - Cyclopentanes

KW - Fertility

KW - Genetic Complementation Test

KW - Intramolecular Oxidoreductases

KW - Molecular Sequence Data

KW - Mutation

KW - Oxylipins

KW - Sequence Homology, Amino Acid

U2 - 10.1007/s00425-002-0906-2

DO - 10.1007/s00425-002-0906-2

M3 - Journal article

C2 - 12430030

VL - 216

SP - 187

EP - 192

JO - Planta

JF - Planta

SN - 0032-0935

IS - 1

ER -

ID: 106391607