The Arabidopsis male-sterile mutant dde2-2 is defective in the ALLENE OXIDE SYNTHASE gene encoding one of the key enzymes of the jasmonic acid biosynthesis pathway
Research output: Contribution to journal › Journal article
The Arabidopsis thaliana (L.) Heynh. mutant delayed-dehiscence2-2 (dde2-2) was identified in an En1/Spm1 transposon-induced mutant population screened for plants showing defects in fertility. The dde2-2 mutant allele is defective in the anther dehiscence process and filament elongation and thus exhibits a male-sterile phenotype. The dde2-2 phenotype can be rescued by application of methyl jasmonate, indicating that the mutant is affected in jasmonic acid biosynthesis. The combination of genetic mapping and a candidate-gene approach identified a frameshift mutation in the ALLENE OXIDE SYNTHASE (AOS) gene, encoding one of the key enzymes of jasmonic acid biosynthesis. Expression analysis and genetic complementation of the dde2-2 phenotype by overexpression of the AOS coding sequence confirmed that the male-sterile phenotype is indeed caused by the mutation in the AOS gene.
Original language | English |
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Journal | Planta |
Volume | 216 |
Issue number | 1 |
Pages (from-to) | 187-92 |
Number of pages | 6 |
ISSN | 0032-0935 |
DOIs | |
Publication status | Published - Nov 2002 |
- Acetates, Amino Acid Sequence, Arabidopsis, Chromosome Mapping, Chromosomes, Plant, Cyclopentanes, Fertility, Genetic Complementation Test, Intramolecular Oxidoreductases, Molecular Sequence Data, Mutation, Oxylipins, Sequence Homology, Amino Acid
Research areas
ID: 106391607