Transient Posttranscriptional Gene Silencing in Medicago truncatula: Virus-Induced Gene Silencing (VIGS)

Research output: Chapter in Book/Report/Conference proceedingBook chapterResearchpeer-review

  • Joanna Serwatowska
  • Ole Søgaard Lund
  • Ida Elisabeth Johansen

Successful application of virus-induced gene silencing for functional genomics requires a virus vector that can initiate a systemic infection of the host plant. Agroinoculation of the pea early browning virus vectors pCAPE1 and pCAPE2 can establish infection in several genotypes of Medicago truncatula and can reduce target gene RNA levels to an extent that allows investigation of gene function.

Original languageEnglish
Title of host publicationFunctional Genomics in Medicago truncatula : Methods and Protocols
EditorsLuis A. Cañas, José Pío Beltrán
Number of pages8
PublisherHumana Press
Publication date2018
ISBN (Print)978-1-4939-8632-3
ISBN (Electronic)978-1-4939-8633-0
Publication statusPublished - 2018
SeriesMethods in Molecular Biology

ID: 210914457