Transient Posttranscriptional Gene Silencing in Medicago truncatula: Virus-Induced Gene Silencing (VIGS)
Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review
Successful application of virus-induced gene silencing for functional genomics requires a virus vector that can initiate a systemic infection of the host plant. Agroinoculation of the pea early browning virus vectors pCAPE1 and pCAPE2 can establish infection in several genotypes of Medicago truncatula and can reduce target gene RNA levels to an extent that allows investigation of gene function.
Original language | English |
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Title of host publication | Functional Genomics in Medicago truncatula : Methods and Protocols |
Editors | Luis A. Cañas, José Pío Beltrán |
Number of pages | 8 |
Volume | 1822 |
Publisher | Humana Press |
Publication date | 2018 |
Pages | 115-122 |
ISBN (Print) | 978-1-4939-8632-3 |
ISBN (Electronic) | 978-1-4939-8633-0 |
DOIs | |
Publication status | Published - 2018 |
Series | Methods in Molecular Biology |
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Volume | 1822 |
ISSN | 1064-3745 |
ID: 210914457