Stigma development and receptivity of two Kalanchoë blossfeldiana cultivars
Research output: Contribution to journal › Journal article › Research › peer-review
Standard
Stigma development and receptivity of two Kalanchoë blossfeldiana cultivars. / Traoré, Leila Thérèse; Kuligowska, Katarzyna; Lütken, Henrik Vlk; Müller, Renate.
In: Acta Physiologiae Plantarum, Vol. 36, No. 7, 2014, p. 1763-1769.Research output: Contribution to journal › Journal article › Research › peer-review
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - Stigma development and receptivity of two Kalanchoë blossfeldiana cultivars
AU - Traoré, Leila Thérèse
AU - Kuligowska, Katarzyna
AU - Lütken, Henrik Vlk
AU - Müller, Renate
PY - 2014
Y1 - 2014
N2 - Several members of the Kalanchoë genus are popular as ornamental plants. Cross-breeding and wide hybridisation are essential to continuously introduce novel traits into cultivated plant material. This study aimed to identify the major factors related to the stigma affecting cross-pollination in the Kalanchoë blossfeldiana. Pollen tube growth after pollination of K. blossfeldiana 'Jackie' and 'Reese' was examined at different stigma developmental stages. Five distinct developmental stages were identified based on changes in morphology and activity of stigmatic peroxidase. After reciprocal pollination at the five stigma developmental stages, fluorescence microscopy was used to estimate the number of pollen tubes in situ. Both cultivars had receptive stigmas from stage I to IV, which concurred with the continuous expansion of the stigma covered with exudates. No pollen tube growth was observed at stage V for both cultivars. The number of pollen tubes was significantly higher in carpels pollinated at stage III, characterized by loose arrangement of the papillae and maximal amount of exudates, compared to all other developmental stages. Stigmas showing drying exudates and absence of peroxidase exhibited a relatively decreased number of pollen tubes in situ. No pollen tubes germinated on wilting stigmas. The arrangement of the papillae, the presence of exudates and peroxidase activity affected the number of pollen tubes in cross-pollination of K. blossfeldiana cultivars 'Jackie' and 'Reese'. These results will help breeders to better select the optimal time for effective pollination. The findings may be applicable for other cultivars of K. blossfeldiana and relevant for different species of Kalanchoë.
AB - Several members of the Kalanchoë genus are popular as ornamental plants. Cross-breeding and wide hybridisation are essential to continuously introduce novel traits into cultivated plant material. This study aimed to identify the major factors related to the stigma affecting cross-pollination in the Kalanchoë blossfeldiana. Pollen tube growth after pollination of K. blossfeldiana 'Jackie' and 'Reese' was examined at different stigma developmental stages. Five distinct developmental stages were identified based on changes in morphology and activity of stigmatic peroxidase. After reciprocal pollination at the five stigma developmental stages, fluorescence microscopy was used to estimate the number of pollen tubes in situ. Both cultivars had receptive stigmas from stage I to IV, which concurred with the continuous expansion of the stigma covered with exudates. No pollen tube growth was observed at stage V for both cultivars. The number of pollen tubes was significantly higher in carpels pollinated at stage III, characterized by loose arrangement of the papillae and maximal amount of exudates, compared to all other developmental stages. Stigmas showing drying exudates and absence of peroxidase exhibited a relatively decreased number of pollen tubes in situ. No pollen tubes germinated on wilting stigmas. The arrangement of the papillae, the presence of exudates and peroxidase activity affected the number of pollen tubes in cross-pollination of K. blossfeldiana cultivars 'Jackie' and 'Reese'. These results will help breeders to better select the optimal time for effective pollination. The findings may be applicable for other cultivars of K. blossfeldiana and relevant for different species of Kalanchoë.
KW - Cross-breeding
KW - Exudates
KW - Papillae
KW - Peroxidase
KW - Pollen tubes
KW - Stigma morphology
U2 - 10.1007/s11738-014-1550-8
DO - 10.1007/s11738-014-1550-8
M3 - Journal article
AN - SCOPUS:84903306359
VL - 36
SP - 1763
EP - 1769
JO - Acta Physiologiae Plantarum
JF - Acta Physiologiae Plantarum
SN - 0137-5881
IS - 7
ER -
ID: 129916917