Root distribution in intercropping systems–a comparison of DNA based methods and visual distinction of roots
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Root distribution in intercropping systems–a comparison of DNA based methods and visual distinction of roots. / Hassan, Affendy; Dresbøll, Dorte Bodin; Rasmussen, Camilla Ruø; Lyhne-Kjærbye, Annemette; Nicolaisen, Mette Haubjerg; Stokholm, Michaela Schiller; Lund, Ole Søgaard; Thorup-Kristensen, Kristian.
In: Archives of Agronomy and Soil Science, Vol. 67, No. 1, 2021, p. 15-28.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Root distribution in intercropping systems–a comparison of DNA based methods and visual distinction of roots
AU - Hassan, Affendy
AU - Dresbøll, Dorte Bodin
AU - Rasmussen, Camilla Ruø
AU - Lyhne-Kjærbye, Annemette
AU - Nicolaisen, Mette Haubjerg
AU - Stokholm, Michaela Schiller
AU - Lund, Ole Søgaard
AU - Thorup-Kristensen, Kristian
PY - 2021
Y1 - 2021
N2 - This study was performed to i) compare root distribution of legume/non-legume intercrops determined by qPCR and amplicon sequencing (Meta-barcoding) to root growth of the individual species determined visually, and ii) compare the qPCR and amplicon sequencing techniques as a method for quantifying root growth of individual species grown in mixtures. Red beet (Beta vulgaris L. cv Detroit) and lucerne (Medicago sativa L. cv Creno) were grown in one-meter-high transparent tubes. Root competition was studied by direct observation on the tube surface determining root intensity (RI), root wash and extraction followed by determination of root length density (RLD), and DNA-based methods. Intercropping decreased lucerne RI strongly, whereas the RI of red beet was not affected. The determination of RLD did not allow us to distinguish species in mixed samples. However, both DNA methods showed apparently a tendency to overestimate the fraction of lucerne roots compared to the direct root observations. The discrepancy between methods was explained by the fact that visual methods provide estimates of root length while the DNA methods provide estimates of root mass. In conclusion, DNA-based estimates of species fractions in mixed root samples give valuable information on root interactions in mixed crops.
AB - This study was performed to i) compare root distribution of legume/non-legume intercrops determined by qPCR and amplicon sequencing (Meta-barcoding) to root growth of the individual species determined visually, and ii) compare the qPCR and amplicon sequencing techniques as a method for quantifying root growth of individual species grown in mixtures. Red beet (Beta vulgaris L. cv Detroit) and lucerne (Medicago sativa L. cv Creno) were grown in one-meter-high transparent tubes. Root competition was studied by direct observation on the tube surface determining root intensity (RI), root wash and extraction followed by determination of root length density (RLD), and DNA-based methods. Intercropping decreased lucerne RI strongly, whereas the RI of red beet was not affected. The determination of RLD did not allow us to distinguish species in mixed samples. However, both DNA methods showed apparently a tendency to overestimate the fraction of lucerne roots compared to the direct root observations. The discrepancy between methods was explained by the fact that visual methods provide estimates of root length while the DNA methods provide estimates of root mass. In conclusion, DNA-based estimates of species fractions in mixed root samples give valuable information on root interactions in mixed crops.
KW - amplicon sequencing
KW - intercropping
KW - qPCR technique
KW - Rhizotron
KW - root growth
U2 - 10.1080/03650340.2019.1675872
DO - 10.1080/03650340.2019.1675872
M3 - Journal article
AN - SCOPUS:85074325213
VL - 67
SP - 15
EP - 28
JO - Archives of Agronomy and Soil Science
JF - Archives of Agronomy and Soil Science
SN - 0365-0340
IS - 1
ER -
ID: 234453347