Physiological phenotyping of mammalian cell lines by enzymatic activity fingerprinting of key carbohydrate metabolic enzymes: a pilot and feasibility study

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Physiological phenotyping of mammalian cell lines by enzymatic activity fingerprinting of key carbohydrate metabolic enzymes : a pilot and feasibility study. / Geiger, Julian; Doelker, Rebecca; Salö, Sofia; Roitsch, Thomas; Dalgaard, Louise T.

In: BMC Research Notes, Vol. 12, 682, 22.10.2019, p. 1-6.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Geiger, J, Doelker, R, Salö, S, Roitsch, T & Dalgaard, LT 2019, 'Physiological phenotyping of mammalian cell lines by enzymatic activity fingerprinting of key carbohydrate metabolic enzymes: a pilot and feasibility study', BMC Research Notes, vol. 12, 682, pp. 1-6. https://doi.org/10.1186/s13104-019-4697-y

APA

Geiger, J., Doelker, R., Salö, S., Roitsch, T., & Dalgaard, L. T. (2019). Physiological phenotyping of mammalian cell lines by enzymatic activity fingerprinting of key carbohydrate metabolic enzymes: a pilot and feasibility study. BMC Research Notes, 12, 1-6. [682]. https://doi.org/10.1186/s13104-019-4697-y

Vancouver

Geiger J, Doelker R, Salö S, Roitsch T, Dalgaard LT. Physiological phenotyping of mammalian cell lines by enzymatic activity fingerprinting of key carbohydrate metabolic enzymes: a pilot and feasibility study. BMC Research Notes. 2019 Oct 22;12:1-6. 682. https://doi.org/10.1186/s13104-019-4697-y

Author

Geiger, Julian ; Doelker, Rebecca ; Salö, Sofia ; Roitsch, Thomas ; Dalgaard, Louise T. / Physiological phenotyping of mammalian cell lines by enzymatic activity fingerprinting of key carbohydrate metabolic enzymes : a pilot and feasibility study. In: BMC Research Notes. 2019 ; Vol. 12. pp. 1-6.

Bibtex

@article{a11e2b7527a84cd8a1ae60491ca826a0,
title = "Physiological phenotyping of mammalian cell lines by enzymatic activity fingerprinting of key carbohydrate metabolic enzymes: a pilot and feasibility study",
abstract = "Objective: Enzymatic fingerprinting of key enzymes of glucose metabolism is a valuable analysis tool in cell physiological phenotyping of plant samples. Yet, a similar approach for mammalian cell line samples is missing. In this study, we applied semi-high throughput enzyme activity assays that were originally designed for plant samples and tested their feasibility in extracts of six frequently used mammalian cell lines (Caco2, HaCaT, C2C12, HEK293, HepG2 and INS-1E). Results: Enzyme activities for aldolase, hexokinase, glucose-6-phosphate dehydrogenase, phosphoglucoisomerase, phosphoglucomutase, phosphofructokinase could be detected in samples of one or more mammalian cell lines. We characterized effects of sample dilution, assay temperature and repeated freeze-thaw cycles causing potential biases. After careful selection of experimental parameters, the presented semi-high throughput methods could be established as useful tool for physiological phenotyping of cultured mammalian cells.",
keywords = "96 well format, Aldolase, Enzyme assays, Glucose-6-phosphate dehydrogenase, Hexokinase, INS-1E, Phosphofructokinase, Phosphoglucoisomerase, Phosphoglucomutase",
author = "Julian Geiger and Rebecca Doelker and Sofia Sal{\"o} and Thomas Roitsch and Dalgaard, {Louise T.}",
year = "2019",
month = oct,
day = "22",
doi = "10.1186/s13104-019-4697-y",
language = "English",
volume = "12",
pages = "1--6",
journal = "BMC Research Notes",
issn = "1756-0500",
publisher = "BioMed Central Ltd.",

}

RIS

TY - JOUR

T1 - Physiological phenotyping of mammalian cell lines by enzymatic activity fingerprinting of key carbohydrate metabolic enzymes

T2 - a pilot and feasibility study

AU - Geiger, Julian

AU - Doelker, Rebecca

AU - Salö, Sofia

AU - Roitsch, Thomas

AU - Dalgaard, Louise T.

PY - 2019/10/22

Y1 - 2019/10/22

N2 - Objective: Enzymatic fingerprinting of key enzymes of glucose metabolism is a valuable analysis tool in cell physiological phenotyping of plant samples. Yet, a similar approach for mammalian cell line samples is missing. In this study, we applied semi-high throughput enzyme activity assays that were originally designed for plant samples and tested their feasibility in extracts of six frequently used mammalian cell lines (Caco2, HaCaT, C2C12, HEK293, HepG2 and INS-1E). Results: Enzyme activities for aldolase, hexokinase, glucose-6-phosphate dehydrogenase, phosphoglucoisomerase, phosphoglucomutase, phosphofructokinase could be detected in samples of one or more mammalian cell lines. We characterized effects of sample dilution, assay temperature and repeated freeze-thaw cycles causing potential biases. After careful selection of experimental parameters, the presented semi-high throughput methods could be established as useful tool for physiological phenotyping of cultured mammalian cells.

AB - Objective: Enzymatic fingerprinting of key enzymes of glucose metabolism is a valuable analysis tool in cell physiological phenotyping of plant samples. Yet, a similar approach for mammalian cell line samples is missing. In this study, we applied semi-high throughput enzyme activity assays that were originally designed for plant samples and tested their feasibility in extracts of six frequently used mammalian cell lines (Caco2, HaCaT, C2C12, HEK293, HepG2 and INS-1E). Results: Enzyme activities for aldolase, hexokinase, glucose-6-phosphate dehydrogenase, phosphoglucoisomerase, phosphoglucomutase, phosphofructokinase could be detected in samples of one or more mammalian cell lines. We characterized effects of sample dilution, assay temperature and repeated freeze-thaw cycles causing potential biases. After careful selection of experimental parameters, the presented semi-high throughput methods could be established as useful tool for physiological phenotyping of cultured mammalian cells.

KW - 96 well format

KW - Aldolase

KW - Enzyme assays

KW - Glucose-6-phosphate dehydrogenase

KW - Hexokinase

KW - INS-1E

KW - Phosphofructokinase

KW - Phosphoglucoisomerase

KW - Phosphoglucomutase

U2 - 10.1186/s13104-019-4697-y

DO - 10.1186/s13104-019-4697-y

M3 - Journal article

C2 - 31640766

AN - SCOPUS:85073747408

VL - 12

SP - 1

EP - 6

JO - BMC Research Notes

JF - BMC Research Notes

SN - 1756-0500

M1 - 682

ER -

ID: 234213741