Modulating the levels of plant hormone cytokinins at the host-pathogen interface

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Standard

Modulating the levels of plant hormone cytokinins at the host-pathogen interface. / Naseem, Muhammad; Shams, Shabana; Roitsch, Thomas Georg.

Auxins and cytokinins in plant biology: methods and protocols. ed. / Thomas Dandekar; Muhammad Naseem. Springer, 2017. p. 141-150 (Methods in Molecular Biology, Vol. 1569).

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Harvard

Naseem, M, Shams, S & Roitsch, TG 2017, Modulating the levels of plant hormone cytokinins at the host-pathogen interface. in T Dandekar & M Naseem (eds), Auxins and cytokinins in plant biology: methods and protocols. Springer, Methods in Molecular Biology, vol. 1569, pp. 141-150. https://doi.org/10.1007/978-1-4939-6831-2_11

APA

Naseem, M., Shams, S., & Roitsch, T. G. (2017). Modulating the levels of plant hormone cytokinins at the host-pathogen interface. In T. Dandekar, & M. Naseem (Eds.), Auxins and cytokinins in plant biology: methods and protocols (pp. 141-150). Springer. Methods in Molecular Biology Vol. 1569 https://doi.org/10.1007/978-1-4939-6831-2_11

Vancouver

Naseem M, Shams S, Roitsch TG. Modulating the levels of plant hormone cytokinins at the host-pathogen interface. In Dandekar T, Naseem M, editors, Auxins and cytokinins in plant biology: methods and protocols. Springer. 2017. p. 141-150. (Methods in Molecular Biology, Vol. 1569). https://doi.org/10.1007/978-1-4939-6831-2_11

Author

Naseem, Muhammad ; Shams, Shabana ; Roitsch, Thomas Georg. / Modulating the levels of plant hormone cytokinins at the host-pathogen interface. Auxins and cytokinins in plant biology: methods and protocols. editor / Thomas Dandekar ; Muhammad Naseem. Springer, 2017. pp. 141-150 (Methods in Molecular Biology, Vol. 1569).

Bibtex

@inbook{8f65b405d6194ea8a0d010c0dc137922,
title = "Modulating the levels of plant hormone cytokinins at the host-pathogen interface",
abstract = "Cytokinins are adenine and non-adenine derived heterogeneous class of regulatory molecules that participate in almost every aspect of plant biology. They also affect plant defense responses as well as help microbial pathogens to establish pathogenesis. The functional approaches that ensure desired and subtle modulations in the levels of plant cytokinins are highly instrumental in assessing their functions in plant immunity. Here, we describe a detailed working protocol regarding the enhanced production of cytokinins from plants that harbor isopentenyltransferase (IPT) enzyme gene under the control of 4xJERE (jasmonic acid and elicitor-responsive element) pathogen-inducible promoter. Our devised expression system is a context-dependent solution when it comes to investigating host-pathogen interactions under the modulated conditions of plant cytokinins.",
keywords = "Journal Article",
author = "Muhammad Naseem and Shabana Shams and Roitsch, {Thomas Georg}",
year = "2017",
doi = "10.1007/978-1-4939-6831-2_11",
language = "English",
isbn = "978-1-4939-6829-9",
series = "Methods in Molecular Biology",
publisher = "Springer",
pages = "141--150",
editor = "Thomas Dandekar and Muhammad Naseem",
booktitle = "Auxins and cytokinins in plant biology",
address = "Switzerland",

}

RIS

TY - CHAP

T1 - Modulating the levels of plant hormone cytokinins at the host-pathogen interface

AU - Naseem, Muhammad

AU - Shams, Shabana

AU - Roitsch, Thomas Georg

PY - 2017

Y1 - 2017

N2 - Cytokinins are adenine and non-adenine derived heterogeneous class of regulatory molecules that participate in almost every aspect of plant biology. They also affect plant defense responses as well as help microbial pathogens to establish pathogenesis. The functional approaches that ensure desired and subtle modulations in the levels of plant cytokinins are highly instrumental in assessing their functions in plant immunity. Here, we describe a detailed working protocol regarding the enhanced production of cytokinins from plants that harbor isopentenyltransferase (IPT) enzyme gene under the control of 4xJERE (jasmonic acid and elicitor-responsive element) pathogen-inducible promoter. Our devised expression system is a context-dependent solution when it comes to investigating host-pathogen interactions under the modulated conditions of plant cytokinins.

AB - Cytokinins are adenine and non-adenine derived heterogeneous class of regulatory molecules that participate in almost every aspect of plant biology. They also affect plant defense responses as well as help microbial pathogens to establish pathogenesis. The functional approaches that ensure desired and subtle modulations in the levels of plant cytokinins are highly instrumental in assessing their functions in plant immunity. Here, we describe a detailed working protocol regarding the enhanced production of cytokinins from plants that harbor isopentenyltransferase (IPT) enzyme gene under the control of 4xJERE (jasmonic acid and elicitor-responsive element) pathogen-inducible promoter. Our devised expression system is a context-dependent solution when it comes to investigating host-pathogen interactions under the modulated conditions of plant cytokinins.

KW - Journal Article

U2 - 10.1007/978-1-4939-6831-2_11

DO - 10.1007/978-1-4939-6831-2_11

M3 - Book chapter

C2 - 28265994

SN - 978-1-4939-6829-9

T3 - Methods in Molecular Biology

SP - 141

EP - 150

BT - Auxins and cytokinins in plant biology

A2 - Dandekar, Thomas

A2 - Naseem, Muhammad

PB - Springer

ER -

ID: 174007792