Investigating the origins and evolution of a glyphosate-resistant weed invasion in South America
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Investigating the origins and evolution of a glyphosate-resistant weed invasion in South America. / Gaines, Todd A.; Slavov, Gancho T.; Hughes, David; Küpper, Anita; Sparks, Crystal D.; Oliva, Julian; Vila-Aiub, Martin M.; Garcia, M. Alejandro; Merotto, Aldo; Neve, Paul.
In: Molecular Ecology, Vol. 30, No. 21, 2021, p. 5360-5372.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Investigating the origins and evolution of a glyphosate-resistant weed invasion in South America
AU - Gaines, Todd A.
AU - Slavov, Gancho T.
AU - Hughes, David
AU - Küpper, Anita
AU - Sparks, Crystal D.
AU - Oliva, Julian
AU - Vila-Aiub, Martin M.
AU - Garcia, M. Alejandro
AU - Merotto, Aldo
AU - Neve, Paul
N1 - Publisher Copyright: © 2021 John Wiley & Sons Ltd
PY - 2021
Y1 - 2021
N2 - The global invasion, and subsequent spread and evolution of weeds provides unique opportunities to address fundamental questions in evolutionary and invasion ecology. Amaranthus palmeri is a widespread glyphosate-resistant (GR) weed in the USA. Since 2015, GR populations of A. palmeri have been confirmed in South America, raising questions about introduction pathways and the importance of pre- vs. post-invasion evolution of GR traits. We used RAD-sequencing genotyping to characterize genetic structure of populations from Brazil, Argentina, Uruguay and the USA. We also quantified gene copy number of the glyphosate target, 5-enolpyruvyl-3-shikimate phosphate synthase (EPSPS), and the presence of an extrachromosomal circular DNA (eccDNA) replicon known to confer glyphosate resistance in USA populations. Populations in Brazil, Argentina and Uruguay were only weakly differentiated (pairwise FST ≤0.043) in comparison to USA populations (mean pairwise FST =0.161, range =0.068–0.258), suggesting a single major invasion event. However, elevated EPSPS copy number and the EPSPS replicon were identified in all populations from Brazil and Uruguay, but only in a single Argentinean population. These observations are consistent with independent in situ evolution of glyphosate resistance in Argentina, followed by some limited recent migration of the eccDNA-based mechanism from Brazil to Argentina. Taken together, our results are consistent with an initial introduction of A. palmeri into South America sometime before the 1980s, and local evolution of GR in Argentina, followed by a secondary invasion of GR A. palmeri with the unique eccDNA-based mechanism from the USA into Brazil and Uruguay during the 2010s.
AB - The global invasion, and subsequent spread and evolution of weeds provides unique opportunities to address fundamental questions in evolutionary and invasion ecology. Amaranthus palmeri is a widespread glyphosate-resistant (GR) weed in the USA. Since 2015, GR populations of A. palmeri have been confirmed in South America, raising questions about introduction pathways and the importance of pre- vs. post-invasion evolution of GR traits. We used RAD-sequencing genotyping to characterize genetic structure of populations from Brazil, Argentina, Uruguay and the USA. We also quantified gene copy number of the glyphosate target, 5-enolpyruvyl-3-shikimate phosphate synthase (EPSPS), and the presence of an extrachromosomal circular DNA (eccDNA) replicon known to confer glyphosate resistance in USA populations. Populations in Brazil, Argentina and Uruguay were only weakly differentiated (pairwise FST ≤0.043) in comparison to USA populations (mean pairwise FST =0.161, range =0.068–0.258), suggesting a single major invasion event. However, elevated EPSPS copy number and the EPSPS replicon were identified in all populations from Brazil and Uruguay, but only in a single Argentinean population. These observations are consistent with independent in situ evolution of glyphosate resistance in Argentina, followed by some limited recent migration of the eccDNA-based mechanism from Brazil to Argentina. Taken together, our results are consistent with an initial introduction of A. palmeri into South America sometime before the 1980s, and local evolution of GR in Argentina, followed by a secondary invasion of GR A. palmeri with the unique eccDNA-based mechanism from the USA into Brazil and Uruguay during the 2010s.
KW - Amaranthus palmeri
KW - herbicide resistance
KW - Palmer amaranth
KW - population genomics
KW - RAD-seq
U2 - 10.1111/mec.16221
DO - 10.1111/mec.16221
M3 - Journal article
C2 - 34637174
AN - SCOPUS:85118353356
VL - 30
SP - 5360
EP - 5372
JO - Molecular Ecology
JF - Molecular Ecology
SN - 0962-1083
IS - 21
ER -
ID: 284634011