Interspecific Hybridisation in Campanula: Establishment of Ovule Culture, Molecular and Morphological Hybrid Characterisation

Research output: Book/Report › Ph.D. thesis › Research

Standard

Interspecific Hybridisation in Campanula : Establishment of Ovule Culture, Molecular and Morphological Hybrid Characterisation . / Röper, Anna Catharina.

Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, 2015. 143 p.

Research output: Book/Report › Ph.D. thesis › Research

Harvard

Röper, AC 2015, Interspecific Hybridisation in Campanula: Establishment of Ovule Culture, Molecular and Morphological Hybrid Characterisation . Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen. <https://soeg.kb.dk/permalink/45KBDK_KGL/fbp0ps/alma99122138672405763>

APA

Röper, A. C. (2015). Interspecific Hybridisation in Campanula: Establishment of Ovule Culture, Molecular and Morphological Hybrid Characterisation . Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen. https://soeg.kb.dk/permalink/45KBDK_KGL/fbp0ps/alma99122138672405763

Vancouver

Röper AC. Interspecific Hybridisation in Campanula: Establishment of Ovule Culture, Molecular and Morphological Hybrid Characterisation . Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, 2015. 143 p.

Author

Röper, Anna Catharina. / Interspecific Hybridisation in Campanula : Establishment of Ovule Culture, Molecular and Morphological Hybrid Characterisation . Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, 2015. 143 p.

Bibtex

@phdthesis{83f5f9af1ce048b781303f3b56aaf4c8,
title = "Interspecific Hybridisation in Campanula: Establishment of Ovule Culture, Molecular and Morphological Hybrid Characterisation ",
abstract = "In the present thesis, economically important Campanula species were selected for interspecific hybridisation to increase the genetic viability in plant breeding material.To reach this goal, ovule culture was established as an embryo rescue technique to overcome post-fertilisation barriers in selected Campanula species. Furthermore, interspecific hybrids with new phenotypes were investigated at the molecular and morphological level.The first research part focused on the effect of different ovule isolation times on selected cultivars from different Campanula species. The ovule isolation time had an influence on the number of ovules and germination success in some cross combinations.In the second research part interspecific hybrids obtained from ovule culture were genotypically and phenotypically characterised by AFLP analysis, flow cytometry and biometrical parameters. Hybridity was confirmed for most of the interspecific hybrids. Intermediate phenotypes compared to the parental species were found for several interspecific hybrids.The results from this PhD study demonstrated that the genetic viability could be increased in Campanula and reveal valuable information for interspecific hybridisation in general",
author = "R{\"o}per, {Anna Catharina}",
year = "2015",
language = "English",
publisher = "Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen",

}

RIS

TY - BOOK

T1 - Interspecific Hybridisation in Campanula

T2 - Establishment of Ovule Culture, Molecular and Morphological Hybrid Characterisation

AU - Röper, Anna Catharina

PY - 2015

Y1 - 2015

N2 - In the present thesis, economically important Campanula species were selected for interspecific hybridisation to increase the genetic viability in plant breeding material.To reach this goal, ovule culture was established as an embryo rescue technique to overcome post-fertilisation barriers in selected Campanula species. Furthermore, interspecific hybrids with new phenotypes were investigated at the molecular and morphological level.The first research part focused on the effect of different ovule isolation times on selected cultivars from different Campanula species. The ovule isolation time had an influence on the number of ovules and germination success in some cross combinations.In the second research part interspecific hybrids obtained from ovule culture were genotypically and phenotypically characterised by AFLP analysis, flow cytometry and biometrical parameters. Hybridity was confirmed for most of the interspecific hybrids. Intermediate phenotypes compared to the parental species were found for several interspecific hybrids.The results from this PhD study demonstrated that the genetic viability could be increased in Campanula and reveal valuable information for interspecific hybridisation in general

AB - In the present thesis, economically important Campanula species were selected for interspecific hybridisation to increase the genetic viability in plant breeding material.To reach this goal, ovule culture was established as an embryo rescue technique to overcome post-fertilisation barriers in selected Campanula species. Furthermore, interspecific hybrids with new phenotypes were investigated at the molecular and morphological level.The first research part focused on the effect of different ovule isolation times on selected cultivars from different Campanula species. The ovule isolation time had an influence on the number of ovules and germination success in some cross combinations.In the second research part interspecific hybrids obtained from ovule culture were genotypically and phenotypically characterised by AFLP analysis, flow cytometry and biometrical parameters. Hybridity was confirmed for most of the interspecific hybrids. Intermediate phenotypes compared to the parental species were found for several interspecific hybrids.The results from this PhD study demonstrated that the genetic viability could be increased in Campanula and reveal valuable information for interspecific hybridisation in general

UR - https://soeg.kb.dk/permalink/45KBDK_KGL/fbp0ps/alma99122138672405763

M3 - Ph.D. thesis

BT - Interspecific Hybridisation in Campanula

PB - Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen

ER -

ID: 140778243