TY - JOUR

T1 - Expression of genes encoding carbohydrate-metabolic enzymes during taproot development in sugar beet (Beta vulgaris subsp. vulgaris)

AU - Jammer, Alexandra

AU - Albacete, Alfonso

AU - Schulz, Britta

AU - Koch, Wolfgang

AU - Weltmeier, Fridtjof

AU - van Der Graaff, Eric

AU - RoitscH, Thomas

PY - 2021

Y1 - 2021

N2 - In sugar beet (Beta vulgaris subsp. vulgaris, Chenopodiaceae), sucrose is stored in a taproot developed by a genus-specific type of anomalous secondary thickening from supernumerary cambial rings. In a previous study (Jammer & al. 2020), we showed that the transition from primary root development to the formation of the sucrose-storing taproot is accompanied by a clear shift in physiological signatures: characteristic changes in the activities of several key enzymes of carbohydrate metabolism were associated with the onset of taproot development and sucrose storage, resulting in three distinct physiological stages (prestorage, transition, secondary growth and sucrose storage). In order to investigate the regulatory mechanisms behind these changes in enzyme activities, we performed a microarray expression analysis for the genes encoding the respective carbohydrate-metabolic enzymes. The data obtained from the microarray were validated for selected transcripts by semi-quantitative reverse transcription PCR. A high level of similarity between the developmental changes in transcript levels and enzyme activities at the transition stage suggests that the majority of the respective enzymes are predominantly regulated on the transcriptional level during the early stages of taproot development.

AB - In sugar beet (Beta vulgaris subsp. vulgaris, Chenopodiaceae), sucrose is stored in a taproot developed by a genus-specific type of anomalous secondary thickening from supernumerary cambial rings. In a previous study (Jammer & al. 2020), we showed that the transition from primary root development to the formation of the sucrose-storing taproot is accompanied by a clear shift in physiological signatures: characteristic changes in the activities of several key enzymes of carbohydrate metabolism were associated with the onset of taproot development and sucrose storage, resulting in three distinct physiological stages (prestorage, transition, secondary growth and sucrose storage). In order to investigate the regulatory mechanisms behind these changes in enzyme activities, we performed a microarray expression analysis for the genes encoding the respective carbohydrate-metabolic enzymes. The data obtained from the microarray were validated for selected transcripts by semi-quantitative reverse transcription PCR. A high level of similarity between the developmental changes in transcript levels and enzyme activities at the transition stage suggests that the majority of the respective enzymes are predominantly regulated on the transcriptional level during the early stages of taproot development.

KW - Beta vulgaris subsp. vulgaris

KW - assimilate partitioning

KW - carbohydrate metabolism

KW - developmental regulation

KW - taproot development

KW - sucrose accumulation

KW - ORGAN-SPECIFIC EXPRESSION

KW - SUCROSE-CLEAVING ENZYMES

KW - INVERTASE ACTIVITY

KW - TRANSLOCATION

KW - ANATOMY

KW - DEFENSE

KW - STORAGE

KW - YIELD

U2 - 10.12905/0380.phyton60-2021-0031

DO - 10.12905/0380.phyton60-2021-0031

M3 - Journal article

VL - 61

SP - 31

EP - 50

JO - Phyton - Annales Rei Botanicae

JF - Phyton - Annales Rei Botanicae

SN - 0079-2047

ER -