Dual labelling by 2H and 15N revealed differences in uptake potential by deep roots of chicory
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Dual labelling by 2H and 15N revealed differences in uptake potential by deep roots of chicory. / Chen, Guanying; Dresbøll, Dorte Bodin; Thorup-Kristensen, Kristian.
In: Rhizosphere, Vol. 19, 100368, 2021.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Dual labelling by 2H and 15N revealed differences in uptake potential by deep roots of chicory
AU - Chen, Guanying
AU - Dresbøll, Dorte Bodin
AU - Thorup-Kristensen, Kristian
N1 - Publisher Copyright: © 2021 The Author(s)
PY - 2021
Y1 - 2021
N2 - Aims: Deep-rooted crops have been widely used in agricultural systems to access deep resources such as water and nitrogen (N). However, the potential of deep roots to take water and N at various depths have not been well studied. Here we used chicory (Cichorium intybus L.) to study the potential and dynamics of water and nitrogen uptake in deep soil layers (below 1 m). Methods: Chicory plants grown in outdoor rhizotrons were labelled by injecting a 2H2O and Ca(15NO3)2 mixture into the soil column at 1.1, 2.3 and 3.5 m depth. Five, ten and twenty days after injection, 2H and 15N were traced in transpiration water and leaves. Results: We found enriched 2H and 15N in water and plant samples, and both water and N uptake were observed down to 3.5 m. The 2H enrichment after injection at 1.1 m depth was 1552‰, almost 10 times higher than after injection at 2.3 m depth, which was 156‰. In contrast, injection at 1.1 and 2.3 m depth resulted in similar 15N enrichment of leaf samples. Conclusion: Deep water uptake was found to be more sensitive to increased depth and reduced root intensity than N uptake, and labelled N was used more rapidly than labelled water. We propose several possible explanations for the discrepancies between deep water and N uptake, and further discuss the challenges of using isotopes and models in deep root studies.
AB - Aims: Deep-rooted crops have been widely used in agricultural systems to access deep resources such as water and nitrogen (N). However, the potential of deep roots to take water and N at various depths have not been well studied. Here we used chicory (Cichorium intybus L.) to study the potential and dynamics of water and nitrogen uptake in deep soil layers (below 1 m). Methods: Chicory plants grown in outdoor rhizotrons were labelled by injecting a 2H2O and Ca(15NO3)2 mixture into the soil column at 1.1, 2.3 and 3.5 m depth. Five, ten and twenty days after injection, 2H and 15N were traced in transpiration water and leaves. Results: We found enriched 2H and 15N in water and plant samples, and both water and N uptake were observed down to 3.5 m. The 2H enrichment after injection at 1.1 m depth was 1552‰, almost 10 times higher than after injection at 2.3 m depth, which was 156‰. In contrast, injection at 1.1 and 2.3 m depth resulted in similar 15N enrichment of leaf samples. Conclusion: Deep water uptake was found to be more sensitive to increased depth and reduced root intensity than N uptake, and labelled N was used more rapidly than labelled water. We propose several possible explanations for the discrepancies between deep water and N uptake, and further discuss the challenges of using isotopes and models in deep root studies.
KW - Dual labelling
KW - Dynamics
KW - Nitrate uptake
KW - Rhizotrons
KW - Root intensity
KW - Water uptake
U2 - 10.1016/j.rhisph.2021.100368
DO - 10.1016/j.rhisph.2021.100368
M3 - Journal article
AN - SCOPUS:85105822452
VL - 19
JO - Rhizosphere
JF - Rhizosphere
SN - 2452-2198
M1 - 100368
ER -
ID: 272643718