TY - JOUR

T1 - Differential response of grapevine to infection with "Candidatus Phytoplasma solani" in early and late growing season through complex regulation of mRNA and small RNA transcriptomes

AU - Dermastia, Marina

AU - Škrlj, Blaž

AU - Strah, Rebeka

AU - Anžič, Barbara

AU - Tomaž, Špela

AU - Križnik, Maja

AU - Schönhuber, Christina

AU - Riedle-bauer, Monika

AU - Ramšak, Živa

AU - Petek, Marko

AU - Kladnik, Aleš

AU - Lavrač, Nada

AU - Gruden, Kristina

AU - Roitsch, Thomas

AU - Brader, Günter

AU - Pompe-novak, Maruša

PY - 2021

Y1 - 2021

N2 - Bois noir is the most widespread phytoplasma grapevine disease in Europe. It is associated with ‘Candidatus Phytoplasma solani’, but molecular interactions between the causal pathogen and its host plant are not well understood. In this work, we combined the analysis of high-throughput RNA- Seq and sRNA-Seq data with interaction network analysis for finding new cross-talks among pathways involved in infection of grapevine cv. Zweigelt with ‘Ca. P. solani’ in early and late growing seasons. While the early growing season was very dynamic at the transcriptional level in asymptomatic grape-vines, the regulation at the level of small RNAs was more pronounced later in the season when symptoms developed in infected grapevines. Most differentially expressed small RNAs were associated with biotic stress. Our study also exposes the less-studied role of hormones in disease development and shows that hormonal balance was already perturbed before symptoms development in infected grapevines. Analysis at the level of communities of genes and mRNA-microRNA interaction networks revealed sev-eral new genes (e.g., expansins and cryptdin) that have not been associated with phytoplasma patho-genicity previously. These novel actors may present a new reference framework for research and diag-nostics of phytoplasma diseases of grapevine.

AB - Bois noir is the most widespread phytoplasma grapevine disease in Europe. It is associated with ‘Candidatus Phytoplasma solani’, but molecular interactions between the causal pathogen and its host plant are not well understood. In this work, we combined the analysis of high-throughput RNA- Seq and sRNA-Seq data with interaction network analysis for finding new cross-talks among pathways involved in infection of grapevine cv. Zweigelt with ‘Ca. P. solani’ in early and late growing seasons. While the early growing season was very dynamic at the transcriptional level in asymptomatic grape-vines, the regulation at the level of small RNAs was more pronounced later in the season when symptoms developed in infected grapevines. Most differentially expressed small RNAs were associated with biotic stress. Our study also exposes the less-studied role of hormones in disease development and shows that hormonal balance was already perturbed before symptoms development in infected grapevines. Analysis at the level of communities of genes and mRNA-microRNA interaction networks revealed sev-eral new genes (e.g., expansins and cryptdin) that have not been associated with phytoplasma patho-genicity previously. These novel actors may present a new reference framework for research and diag-nostics of phytoplasma diseases of grapevine.

KW - Bois noir 3

KW - Candidatus Phytoplasma solani 1

KW - Grapevine 2

KW - Hormones 8

KW - Interaction network 9

KW - MiRNA 6

KW - PhasiRNA 7

KW - RNA-Seq 4

KW - SRNA-Seq 5

U2 - 10.3390/ijms22073531

DO - 10.3390/ijms22073531

M3 - Journal article

C2 - 33805429

AN - SCOPUS:85103098482

VL - 22

JO - International Journal of Molecular Sciences (CD-ROM)

JF - International Journal of Molecular Sciences (CD-ROM)

SN - 1424-6783

IS - 7

M1 - 3531

ER -