Determination of the activity signature of key carbohydrate metabolism enzymes in phenolic-rich grapevine tissues
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Determination of the activity signature of key carbohydrate metabolism enzymes in phenolic-rich grapevine tissues. / Covington, Elizabeth Dunn; Roitsch, Thomas Georg; Dermastia, Marina.
In: Acta Chimica Slovenica, Vol. 63, No. 4, 2016, p. 757-762.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Determination of the activity signature of key carbohydrate metabolism enzymes in phenolic-rich grapevine tissues
AU - Covington, Elizabeth Dunn
AU - Roitsch, Thomas Georg
AU - Dermastia, Marina
PY - 2016
Y1 - 2016
N2 - Physiological studies in plants often require enzyme extraction from tissues containing high concentrations of phenols and polyphenols. Unless removed or neutralized, such compounds may hinder extraction, inactivate enzymes, and interfere with enzyme detection. The following protocol for activity assays for enzymes of primary carbohydrate metabolism, while based on our recently published one for quantitative measurement of activities using coupled spectrophotometric assays in a 96-well format, is tailored to the complexities of phenolic- and anthocyanin-rich extracts from grapevine leaf. As a case study we applied the protocol to grapevine leaf samples infected with plant pathogenic bacteria 'Candidatus Phytoplasma solani', known to alter carbohydrate metabolism in grapevine. The described adaptations may be useful for determination of metabolic fingerprints for physiological phenotyping of other plant species with inherently high levels of phenolic compounds.
AB - Physiological studies in plants often require enzyme extraction from tissues containing high concentrations of phenols and polyphenols. Unless removed or neutralized, such compounds may hinder extraction, inactivate enzymes, and interfere with enzyme detection. The following protocol for activity assays for enzymes of primary carbohydrate metabolism, while based on our recently published one for quantitative measurement of activities using coupled spectrophotometric assays in a 96-well format, is tailored to the complexities of phenolic- and anthocyanin-rich extracts from grapevine leaf. As a case study we applied the protocol to grapevine leaf samples infected with plant pathogenic bacteria 'Candidatus Phytoplasma solani', known to alter carbohydrate metabolism in grapevine. The described adaptations may be useful for determination of metabolic fingerprints for physiological phenotyping of other plant species with inherently high levels of phenolic compounds.
KW - AGPase
KW - Carbohydrates
KW - Invertases
KW - Panel of enzyme activity assays
KW - Phytoplasma
KW - Sucrose synthase
U2 - 10.17344/acsi.2016.2484
DO - 10.17344/acsi.2016.2484
M3 - Journal article
AN - SCOPUS:85003875024
VL - 63
SP - 757
EP - 762
JO - Acta Chimica Slovenica
JF - Acta Chimica Slovenica
SN - 1318-0207
IS - 4
ER -
ID: 172029535