Biochemical and molecular tools reveal two diverse Xanthomonas groups in bananas

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Biochemical and molecular tools reveal two diverse Xanthomonas groups in bananas. / Adriko, John; Aritua, V.; Mortensen, Carmen Nieves; Tushemereirwe, W.K.; Mulondo, A.L.; Kubiriba, J.; Lund, Ole Søgaard.

In: Microbiological Research, Vol. 183, 2016, p. 109-116.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Adriko, J, Aritua, V, Mortensen, CN, Tushemereirwe, WK, Mulondo, AL, Kubiriba, J & Lund, OS 2016, 'Biochemical and molecular tools reveal two diverse Xanthomonas groups in bananas', Microbiological Research, vol. 183, pp. 109-116. https://doi.org/10.1016/j.micres.2015.12.002

APA

Adriko, J., Aritua, V., Mortensen, C. N., Tushemereirwe, W. K., Mulondo, A. L., Kubiriba, J., & Lund, O. S. (2016). Biochemical and molecular tools reveal two diverse Xanthomonas groups in bananas. Microbiological Research, 183, 109-116. https://doi.org/10.1016/j.micres.2015.12.002

Vancouver

Adriko J, Aritua V, Mortensen CN, Tushemereirwe WK, Mulondo AL, Kubiriba J et al. Biochemical and molecular tools reveal two diverse Xanthomonas groups in bananas. Microbiological Research. 2016;183:109-116. https://doi.org/10.1016/j.micres.2015.12.002

Author

Adriko, John ; Aritua, V. ; Mortensen, Carmen Nieves ; Tushemereirwe, W.K. ; Mulondo, A.L. ; Kubiriba, J. ; Lund, Ole Søgaard. / Biochemical and molecular tools reveal two diverse Xanthomonas groups in bananas. In: Microbiological Research. 2016 ; Vol. 183. pp. 109-116.

Bibtex

@article{92a5dacf0ea84723b0829c5e5a5945b4,
title = "Biochemical and molecular tools reveal two diverse Xanthomonas groups in bananas",
abstract = "Xanthomonas campestris pv. musacearum (Xcm) causing the banana Xanthomonas wilt (BXW) disease has been the main xanthomonad associated with bananas in East and Central Africa based on phenotypic and biochemical characteristics. However, biochemical methods cannot effectively distinguish between pathogenic and non-pathogenic xanthomonads. In this study, gram-negative and yellow-pigmented mucoid bacteria were isolated from BXW symptomatic and symptomless bananas collected from different parts of Uganda. Biolog, Xcm-specific (GspDm), Xanthomonas vasicola species-specific (NZ085) and Xanthomonas genus-specific (X1623) primers in PCR, and sequencing of ITS region were used to identify and characterize the isolates. Biolog tests revealed several isolates as xanthomonads. The GspDm and NZ085 primers accurately identified three isolates from diseased bananas as Xcm and these were pathogenic when re-inoculated into bananas. DNA from more isolates than those amplified by GspDm and NZ085 primers were amplified by the X1623 primers implying they are xanthomonads, these were however non-pathogenic on bananas. In the 16-23 ITS sequence based phylogeny, the pathogenic bacteria clustered together with the Xcm reference strain, while the non-pathogenic xanthomonads isolated from both BXW symptomatic and symptomless bananas clustered with group I xanthomonads. The findings reveal dynamic Xanthomonas populations in bananas, which can easily be misrepresented by only using phenotyping and biochemical tests. A combination of tools provides the most accurate identity and characterization of these plant associated bacteria. The interactions between the pathogenic and non-pathogenic xanthomonads in bananas may pave way to understanding effect of microbial interactions on BXW disease development and offer clues to biocontrol of Xcm.",
keywords = "Journal Article, Research Support, Non-U.S. Gov't",
author = "John Adriko and V. Aritua and Mortensen, {Carmen Nieves} and W.K. Tushemereirwe and A.L. Mulondo and J. Kubiriba and Lund, {Ole S{\o}gaard}",
note = "Copyright {\textcopyright} 2016. Published by Elsevier GmbH.",
year = "2016",
doi = "10.1016/j.micres.2015.12.002",
language = "English",
volume = "183",
pages = "109--116",
journal = "Microbiological Research",
issn = "0944-5013",
publisher = "Elsevier GmbH - Urban und Fischer",

}

RIS

TY - JOUR

T1 - Biochemical and molecular tools reveal two diverse Xanthomonas groups in bananas

AU - Adriko, John

AU - Aritua, V.

AU - Mortensen, Carmen Nieves

AU - Tushemereirwe, W.K.

AU - Mulondo, A.L.

AU - Kubiriba, J.

AU - Lund, Ole Søgaard

N1 - Copyright © 2016. Published by Elsevier GmbH.

PY - 2016

Y1 - 2016

N2 - Xanthomonas campestris pv. musacearum (Xcm) causing the banana Xanthomonas wilt (BXW) disease has been the main xanthomonad associated with bananas in East and Central Africa based on phenotypic and biochemical characteristics. However, biochemical methods cannot effectively distinguish between pathogenic and non-pathogenic xanthomonads. In this study, gram-negative and yellow-pigmented mucoid bacteria were isolated from BXW symptomatic and symptomless bananas collected from different parts of Uganda. Biolog, Xcm-specific (GspDm), Xanthomonas vasicola species-specific (NZ085) and Xanthomonas genus-specific (X1623) primers in PCR, and sequencing of ITS region were used to identify and characterize the isolates. Biolog tests revealed several isolates as xanthomonads. The GspDm and NZ085 primers accurately identified three isolates from diseased bananas as Xcm and these were pathogenic when re-inoculated into bananas. DNA from more isolates than those amplified by GspDm and NZ085 primers were amplified by the X1623 primers implying they are xanthomonads, these were however non-pathogenic on bananas. In the 16-23 ITS sequence based phylogeny, the pathogenic bacteria clustered together with the Xcm reference strain, while the non-pathogenic xanthomonads isolated from both BXW symptomatic and symptomless bananas clustered with group I xanthomonads. The findings reveal dynamic Xanthomonas populations in bananas, which can easily be misrepresented by only using phenotyping and biochemical tests. A combination of tools provides the most accurate identity and characterization of these plant associated bacteria. The interactions between the pathogenic and non-pathogenic xanthomonads in bananas may pave way to understanding effect of microbial interactions on BXW disease development and offer clues to biocontrol of Xcm.

AB - Xanthomonas campestris pv. musacearum (Xcm) causing the banana Xanthomonas wilt (BXW) disease has been the main xanthomonad associated with bananas in East and Central Africa based on phenotypic and biochemical characteristics. However, biochemical methods cannot effectively distinguish between pathogenic and non-pathogenic xanthomonads. In this study, gram-negative and yellow-pigmented mucoid bacteria were isolated from BXW symptomatic and symptomless bananas collected from different parts of Uganda. Biolog, Xcm-specific (GspDm), Xanthomonas vasicola species-specific (NZ085) and Xanthomonas genus-specific (X1623) primers in PCR, and sequencing of ITS region were used to identify and characterize the isolates. Biolog tests revealed several isolates as xanthomonads. The GspDm and NZ085 primers accurately identified three isolates from diseased bananas as Xcm and these were pathogenic when re-inoculated into bananas. DNA from more isolates than those amplified by GspDm and NZ085 primers were amplified by the X1623 primers implying they are xanthomonads, these were however non-pathogenic on bananas. In the 16-23 ITS sequence based phylogeny, the pathogenic bacteria clustered together with the Xcm reference strain, while the non-pathogenic xanthomonads isolated from both BXW symptomatic and symptomless bananas clustered with group I xanthomonads. The findings reveal dynamic Xanthomonas populations in bananas, which can easily be misrepresented by only using phenotyping and biochemical tests. A combination of tools provides the most accurate identity and characterization of these plant associated bacteria. The interactions between the pathogenic and non-pathogenic xanthomonads in bananas may pave way to understanding effect of microbial interactions on BXW disease development and offer clues to biocontrol of Xcm.

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1016/j.micres.2015.12.002

DO - 10.1016/j.micres.2015.12.002

M3 - Journal article

C2 - 26805624

VL - 183

SP - 109

EP - 116

JO - Microbiological Research

JF - Microbiological Research

SN - 0944-5013

ER -

ID: 169136822