Allopolyploidization in Cucumis contributes to delayed leaf maturation with repression of redundant homoeologous genes
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Allopolyploidization in Cucumis contributes to delayed leaf maturation with repression of redundant homoeologous genes. / Yu, Xiaqing; Wang, Xixi; Hyldgaard, Benita; Zhu, Zaobing; Zhou, Rong; Kjær, Katrine Heinsvig; Ouzounis, Theoharis; Lou, Qunfeng; Li, Ji; Cai, Qingsheng; Rosenqvist, Eva; Ottosen, Carl Otto; Chen, Jinfeng.
In: Plant Journal, Vol. 94, No. 2, 2018, p. 393-404.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Allopolyploidization in Cucumis contributes to delayed leaf maturation with repression of redundant homoeologous genes
AU - Yu, Xiaqing
AU - Wang, Xixi
AU - Hyldgaard, Benita
AU - Zhu, Zaobing
AU - Zhou, Rong
AU - Kjær, Katrine Heinsvig
AU - Ouzounis, Theoharis
AU - Lou, Qunfeng
AU - Li, Ji
AU - Cai, Qingsheng
AU - Rosenqvist, Eva
AU - Ottosen, Carl Otto
AU - Chen, Jinfeng
PY - 2018
Y1 - 2018
N2 - The important role of polyploidy in plant evolution is widely recognized. However, many questions remain to be explored to address how polyploidy affects the phenotype of the plant. To shed light on the phenotypic and molecular impacts of allopolyploidy, we investigated the leaf development of a synthesized allotetraploid (Cucumis × hytivus), with an emphasis on chlorophyll development. Delayed leaf maturation was identified in C. × hytivus, based on delayed leaf expansion, initial chlorophyll deficiency in the leaves and disordered sink-source transition. Anatomical observations also revealed disturbed chloroplast development in C. ×hytivus. The determination of chlorophyll biosynthesis intermediates suggested that the chlorophyll biosynthesis pathway of C. × hytivus is blocked at the site at which uroporphyrinogen III is catalysed to coproporphyrinogen III. Three chlorophyll biosynthesis-related genes, HEMA1, HEME2 and POR, were significantly repressed in C. × hytivus. Sequence alignment showed both synonymous and non-synonymous substitutions in the HEMA1, HEME2 and POR genes of the parents. Cloning of the chlorophyll biosynthetic genes suggested the retention of homoeologs. In addition, a chimeric clone of the HEMA1 gene that consisted of homologous genes from the parents was identified in C. × hytivus. Overall, our results showed that allopolyploidization in Cucumis has resulted in disturbed chloroplast development and reduced chlorophyll biosynthesis caused by the repressed expression of duplicated homologous genes, which further led to delayed leaf maturation in the allotetraploid, C. × hytivus. The preferential retention/loss of certain types of genes and non-reciprocal homoeologous recombination were also supported in the present study, which provides new insights into the impact of allopolyploidy.
AB - The important role of polyploidy in plant evolution is widely recognized. However, many questions remain to be explored to address how polyploidy affects the phenotype of the plant. To shed light on the phenotypic and molecular impacts of allopolyploidy, we investigated the leaf development of a synthesized allotetraploid (Cucumis × hytivus), with an emphasis on chlorophyll development. Delayed leaf maturation was identified in C. × hytivus, based on delayed leaf expansion, initial chlorophyll deficiency in the leaves and disordered sink-source transition. Anatomical observations also revealed disturbed chloroplast development in C. ×hytivus. The determination of chlorophyll biosynthesis intermediates suggested that the chlorophyll biosynthesis pathway of C. × hytivus is blocked at the site at which uroporphyrinogen III is catalysed to coproporphyrinogen III. Three chlorophyll biosynthesis-related genes, HEMA1, HEME2 and POR, were significantly repressed in C. × hytivus. Sequence alignment showed both synonymous and non-synonymous substitutions in the HEMA1, HEME2 and POR genes of the parents. Cloning of the chlorophyll biosynthetic genes suggested the retention of homoeologs. In addition, a chimeric clone of the HEMA1 gene that consisted of homologous genes from the parents was identified in C. × hytivus. Overall, our results showed that allopolyploidization in Cucumis has resulted in disturbed chloroplast development and reduced chlorophyll biosynthesis caused by the repressed expression of duplicated homologous genes, which further led to delayed leaf maturation in the allotetraploid, C. × hytivus. The preferential retention/loss of certain types of genes and non-reciprocal homoeologous recombination were also supported in the present study, which provides new insights into the impact of allopolyploidy.
KW - allopolyploidy
KW - chlorophyll
KW - Cucumis
KW - delayed maturity
KW - homoeolog
KW - recombination
KW - retention
U2 - 10.1111/tpj.13865
DO - 10.1111/tpj.13865
M3 - Journal article
C2 - 29421854
AN - SCOPUS:85044230889
VL - 94
SP - 393
EP - 404
JO - Plant Journal
JF - Plant Journal
SN - 0960-7412
IS - 2
ER -
ID: 201043800