Activation tagging of the two closely linked genes LEP and VAS independently affects vascular cell number

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Activation tagging of the two closely linked genes LEP and VAS independently affects vascular cell number. / van der Graaff, Eric; Hooykaas, Paul J J; Keller, Beat.

In: Plant Journal, Vol. 32, No. 5, 12.2002, p. 819-30.

Research output: Contribution to journal › Journal article › Research

Harvard

van der Graaff, E, Hooykaas, PJJ & Keller, B 2002, 'Activation tagging of the two closely linked genes LEP and VAS independently affects vascular cell number', Plant Journal, vol. 32, no. 5, pp. 819-30.

APA

van der Graaff, E., Hooykaas, P. J. J., & Keller, B. (2002). Activation tagging of the two closely linked genes LEP and VAS independently affects vascular cell number. Plant Journal, 32(5), 819-30.

Vancouver

van der Graaff E, Hooykaas PJJ, Keller B. Activation tagging of the two closely linked genes LEP and VAS independently affects vascular cell number. Plant Journal. 2002 Dec;32(5):819-30.

Author

van der Graaff, Eric ; Hooykaas, Paul J J ; Keller, Beat. / Activation tagging of the two closely linked genes LEP and VAS independently affects vascular cell number. In: Plant Journal. 2002 ; Vol. 32, No. 5. pp. 819-30.

Bibtex

@article{c193613915c14d2683ea349b4439dcae,
title = "Activation tagging of the two closely linked genes LEP and VAS independently affects vascular cell number",
abstract = "The complex dominant Arabidopsis thaliana mutant lettuce (let) shows the conversion of the leaf petiole into a leaf blade caused by an ectopic leaf blade formation. This is the result of the activation tagging of the LEAFY PETIOLE (LEP) gene encoding an AP2/EREBP-like transcription factor. Here, we report that in addition to this leafy petiole phenotype, the size of the vascular bundles is increased in all aerial organs in let as a result of an increase in the number of xylem, phloem (pro)cambial and pericycle cells. This vascular phenotype is caused by activation tagging of the two genes VASCULAR TISSUE SIZE (VAS) and LEP. These genes are closely linked and arranged in tandem. Activation tagging of LEP only caused a specific increase in the number of xylem cells. This increased xylem cell number, together with the ectopic leaf blade formation, indicates that LEP functions as a cell division-promoting factor. The activation tagging of VAS only resulted in a specific increase in phloem (pro)cambial and pericycle cells. We conclude that activation tagging of LEP and VAS results in additive phenotypes. Insertional mutants for LEP and VAS display wild-type vascular development, indicating the relevance of activation tagging for functional analysis of novel genes involved in plant development.",
keywords = "Amino Acid Sequence, Arabidopsis, Arabidopsis Proteins, Base Sequence, Cell Count, Cell Division, Gene Expression Regulation, Plant, Genes, Plant, Hypocotyl, Phenotype, Plant Leaves, Transcription Factors, Transgenes",
author = "{van der Graaff}, Eric and Hooykaas, {Paul J J} and Beat Keller",
year = "2002",
month = dec,
language = "English",
volume = "32",
pages = "819--30",
journal = "Plant Journal",
issn = "0960-7412",
publisher = "Wiley-Blackwell",
number = "5",

}

RIS

TY - JOUR

T1 - Activation tagging of the two closely linked genes LEP and VAS independently affects vascular cell number

AU - van der Graaff, Eric

AU - Hooykaas, Paul J J

AU - Keller, Beat

PY - 2002/12

Y1 - 2002/12

N2 - The complex dominant Arabidopsis thaliana mutant lettuce (let) shows the conversion of the leaf petiole into a leaf blade caused by an ectopic leaf blade formation. This is the result of the activation tagging of the LEAFY PETIOLE (LEP) gene encoding an AP2/EREBP-like transcription factor. Here, we report that in addition to this leafy petiole phenotype, the size of the vascular bundles is increased in all aerial organs in let as a result of an increase in the number of xylem, phloem (pro)cambial and pericycle cells. This vascular phenotype is caused by activation tagging of the two genes VASCULAR TISSUE SIZE (VAS) and LEP. These genes are closely linked and arranged in tandem. Activation tagging of LEP only caused a specific increase in the number of xylem cells. This increased xylem cell number, together with the ectopic leaf blade formation, indicates that LEP functions as a cell division-promoting factor. The activation tagging of VAS only resulted in a specific increase in phloem (pro)cambial and pericycle cells. We conclude that activation tagging of LEP and VAS results in additive phenotypes. Insertional mutants for LEP and VAS display wild-type vascular development, indicating the relevance of activation tagging for functional analysis of novel genes involved in plant development.

AB - The complex dominant Arabidopsis thaliana mutant lettuce (let) shows the conversion of the leaf petiole into a leaf blade caused by an ectopic leaf blade formation. This is the result of the activation tagging of the LEAFY PETIOLE (LEP) gene encoding an AP2/EREBP-like transcription factor. Here, we report that in addition to this leafy petiole phenotype, the size of the vascular bundles is increased in all aerial organs in let as a result of an increase in the number of xylem, phloem (pro)cambial and pericycle cells. This vascular phenotype is caused by activation tagging of the two genes VASCULAR TISSUE SIZE (VAS) and LEP. These genes are closely linked and arranged in tandem. Activation tagging of LEP only caused a specific increase in the number of xylem cells. This increased xylem cell number, together with the ectopic leaf blade formation, indicates that LEP functions as a cell division-promoting factor. The activation tagging of VAS only resulted in a specific increase in phloem (pro)cambial and pericycle cells. We conclude that activation tagging of LEP and VAS results in additive phenotypes. Insertional mutants for LEP and VAS display wild-type vascular development, indicating the relevance of activation tagging for functional analysis of novel genes involved in plant development.

KW - Amino Acid Sequence

KW - Arabidopsis

KW - Arabidopsis Proteins

KW - Base Sequence

KW - Cell Count

KW - Cell Division

KW - Gene Expression Regulation, Plant

KW - Genes, Plant

KW - Hypocotyl

KW - Phenotype

KW - Plant Leaves

KW - Transcription Factors

KW - Transgenes

M3 - Journal article

C2 - 12472696

VL - 32

SP - 819

EP - 830

JO - Plant Journal

JF - Plant Journal

SN - 0960-7412

IS - 5

ER -

ID: 106391552