NRT/PTR transporters are essential for translocation of glucosinolate defence compounds to seeds
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NRT/PTR transporters are essential for translocation of glucosinolate defence compounds to seeds. / Nour-Eldin, Hussam Hassan; Andersen, Tonni Grube; Burow, Meike; Madsen, Svend Roesen; Jørgensen, Morten Egevang; Olsen, Carl Erik; Dreyer, Ingo; Hedrich, Rainer; Geiger, Dietmar; Halkier, Barbara Ann.
In: Nature, Vol. 488, No. 7412, 2012, p. 531-534.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - NRT/PTR transporters are essential for translocation of glucosinolate defence compounds to seeds
AU - Nour-Eldin, Hussam Hassan
AU - Andersen, Tonni Grube
AU - Burow, Meike
AU - Madsen, Svend Roesen
AU - Jørgensen, Morten Egevang
AU - Olsen, Carl Erik
AU - Dreyer, Ingo
AU - Hedrich, Rainer
AU - Geiger, Dietmar
AU - Halkier, Barbara Ann
PY - 2012
Y1 - 2012
N2 - In plants, transport processes are important for the reallocation of defence compounds to protect tissues of high value, as demonstrated in the plant model Arabidopsis, in which the major defence compounds, glucosinolates, are translocated to seeds on maturation. The molecular basis for long-distance transport of glucosinolates and other defence compounds, however, remains unknown. Here we identify and characterize two members of the nitrate/peptide transporter family, GTR1 and GTR2, as high-affinity, proton-dependent glucosinolate-specific transporters. The gtr1¿gtr2 double mutant did not accumulate glucosinolates in seeds and had more than tenfold over-accumulation in source tissues such as leaves and silique walls, indicating that both plasma membrane-localized transporters are essential for long-distance transport of glucosinolates. We propose that GTR1 and GTR2 control the loading of glucosinolates from the apoplasm into the phloem. Identification of the glucosinolate transporters has agricultural potential as a means to control allocation of defence compounds in a tissue-specific manner.
AB - In plants, transport processes are important for the reallocation of defence compounds to protect tissues of high value, as demonstrated in the plant model Arabidopsis, in which the major defence compounds, glucosinolates, are translocated to seeds on maturation. The molecular basis for long-distance transport of glucosinolates and other defence compounds, however, remains unknown. Here we identify and characterize two members of the nitrate/peptide transporter family, GTR1 and GTR2, as high-affinity, proton-dependent glucosinolate-specific transporters. The gtr1¿gtr2 double mutant did not accumulate glucosinolates in seeds and had more than tenfold over-accumulation in source tissues such as leaves and silique walls, indicating that both plasma membrane-localized transporters are essential for long-distance transport of glucosinolates. We propose that GTR1 and GTR2 control the loading of glucosinolates from the apoplasm into the phloem. Identification of the glucosinolate transporters has agricultural potential as a means to control allocation of defence compounds in a tissue-specific manner.
KW - Animals
KW - Arabidopsis
KW - Arabidopsis Proteins
KW - Biological Transport
KW - Cell Extracts
KW - Evolution, Molecular
KW - Gene Deletion
KW - Gene Library
KW - Genes, Plant
KW - Glucosinolates
KW - Monosaccharide Transport Proteins
KW - Oocytes
KW - Organ Specificity
KW - Phloem
KW - Protons
KW - Seeds
KW - Xenopus laevis
U2 - 10.1038/nature11285
DO - 10.1038/nature11285
M3 - Journal article
C2 - 22864417
VL - 488
SP - 531
EP - 534
JO - Nature
JF - Nature
SN - 0028-0836
IS - 7412
ER -
ID: 40925058