TY - ABST

T1 - Harnessing beneficial microbes from Danish natural soils to biostimulate plant nutrition

AU - Xu, Deyang

AU - Dethier, Laura

AU - Halkier, Barbara Ann

PY - 2022/11/13

Y1 - 2022/11/13

N2 - Under natural environment, plants growing in low-nutrient soils areoften associated with microbes that can promote nutrient acquisition.However, the plant signal cascades linking the response tonutrient deficiency with the establishment of beneficial interactionsremains elusive. Here we use Arabidopsis thaliana growing in naturalsoils that vary in nutrient level as a model system to study themolecular mechanism of microbe recruitment. As a new initiative,we screened soils for beneficial microbes that contribute to plantnutrition across a wide range of Danish habitats.Our studies show that soil samples collected from four sites containmicrobes that ensure the maintenance of magnesium, phosphorus,and sulfur levels in mature rosette leaves of Arabidopsis Col-0 (unpubl.data). By comparing plant-associated microbiota with microbiotafrom bulk soil, we identified many root-enriched operationaltaxonomic units (OTUs). To target the root microbial consortiaresponsible for plant nutrition, we developed a plant transplantation-based method and showed that the endophytic microbes aresufficient for maintaining magnesium level in plants growing in onesite. Identification of beneficial microbes will focus on endophyticmicrobial culture.In summary, combining the phenotype-based screening with thetransplantation experiments simplify the workflow for culture-dependentidentification of beneficial microbes required for plant nutritionand paves the way for investigation of the mechanism of theestablishment of the beneficial community.

AB - Under natural environment, plants growing in low-nutrient soils areoften associated with microbes that can promote nutrient acquisition.However, the plant signal cascades linking the response tonutrient deficiency with the establishment of beneficial interactionsremains elusive. Here we use Arabidopsis thaliana growing in naturalsoils that vary in nutrient level as a model system to study themolecular mechanism of microbe recruitment. As a new initiative,we screened soils for beneficial microbes that contribute to plantnutrition across a wide range of Danish habitats.Our studies show that soil samples collected from four sites containmicrobes that ensure the maintenance of magnesium, phosphorus,and sulfur levels in mature rosette leaves of Arabidopsis Col-0 (unpubl.data). By comparing plant-associated microbiota with microbiotafrom bulk soil, we identified many root-enriched operationaltaxonomic units (OTUs). To target the root microbial consortiaresponsible for plant nutrition, we developed a plant transplantation-based method and showed that the endophytic microbes aresufficient for maintaining magnesium level in plants growing in onesite. Identification of beneficial microbes will focus on endophyticmicrobial culture.In summary, combining the phenotype-based screening with thetransplantation experiments simplify the workflow for culture-dependentidentification of beneficial microbes required for plant nutritionand paves the way for investigation of the mechanism of theestablishment of the beneficial community.

M3 - Conference abstract for conference

T2 - Copenhagen Bioscience Conference: Plant-Microbe Interactions

Y2 - 13 November 2022 through 17 November 2022

ER -