Removal of Polysorbate 80 by complexation prior to LC-MS analysis

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Removal of Polysorbate 80 by complexation prior to LC-MS analysis. / Jäpelt, Kristina Bak; Johnsen, Lea Giørtz; Christensen, Jan H.

In: Analusis - European Journal of Analytical Chemistry, Vol. 408, No. 9, 2016, p. 2303-2307.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Jäpelt, KB, Johnsen, LG & Christensen, JH 2016, 'Removal of Polysorbate 80 by complexation prior to LC-MS analysis', Analusis - European Journal of Analytical Chemistry, vol. 408, no. 9, pp. 2303-2307. https://doi.org/10.1007/s00216-016-9326-1

APA

Jäpelt, K. B., Johnsen, L. G., & Christensen, J. H. (2016). Removal of Polysorbate 80 by complexation prior to LC-MS analysis. Analusis - European Journal of Analytical Chemistry, 408(9), 2303-2307. https://doi.org/10.1007/s00216-016-9326-1

Vancouver

Jäpelt KB, Johnsen LG, Christensen JH. Removal of Polysorbate 80 by complexation prior to LC-MS analysis. Analusis - European Journal of Analytical Chemistry. 2016;408(9):2303-2307. https://doi.org/10.1007/s00216-016-9326-1

Author

Jäpelt, Kristina Bak ; Johnsen, Lea Giørtz ; Christensen, Jan H. / Removal of Polysorbate 80 by complexation prior to LC-MS analysis. In: Analusis - European Journal of Analytical Chemistry. 2016 ; Vol. 408, No. 9. pp. 2303-2307.

Bibtex

@article{e292d3a178304dc7bd47a758efd59910,
title = "Removal of Polysorbate 80 by complexation prior to LC-MS analysis",
abstract = "The presence of Polysorbate 80 in samples can challenge liquid chromatography-electrospray ionisation mass spectrometry (LC-ESI-MS) analysis as it is easily ionised and detected. In this study, we demonstrate that interference from Polysorbate 80 can be reduced by complexation with a metal ion followed by precipitation by thiocyanate. The precipitation procedure was tested on a mixture of low molecular weight compounds (e.g. amino acids and non-amino organic acids) and it was shown that none of the tested compounds were precipitated.",
keywords = "Journal Article, Research Support, Non-U.S. Gov't",
author = "J{\"a}pelt, {Kristina Bak} and Johnsen, {Lea Gi{\o}rtz} and Christensen, {Jan H.}",
year = "2016",
doi = "10.1007/s00216-016-9326-1",
language = "English",
volume = "408",
pages = "2303--2307",
journal = "Analusis",
issn = "0365-4877",
publisher = "EDP Sciences",
number = "9",

}

RIS

TY - JOUR

T1 - Removal of Polysorbate 80 by complexation prior to LC-MS analysis

AU - Jäpelt, Kristina Bak

AU - Johnsen, Lea Giørtz

AU - Christensen, Jan H.

PY - 2016

Y1 - 2016

N2 - The presence of Polysorbate 80 in samples can challenge liquid chromatography-electrospray ionisation mass spectrometry (LC-ESI-MS) analysis as it is easily ionised and detected. In this study, we demonstrate that interference from Polysorbate 80 can be reduced by complexation with a metal ion followed by precipitation by thiocyanate. The precipitation procedure was tested on a mixture of low molecular weight compounds (e.g. amino acids and non-amino organic acids) and it was shown that none of the tested compounds were precipitated.

AB - The presence of Polysorbate 80 in samples can challenge liquid chromatography-electrospray ionisation mass spectrometry (LC-ESI-MS) analysis as it is easily ionised and detected. In this study, we demonstrate that interference from Polysorbate 80 can be reduced by complexation with a metal ion followed by precipitation by thiocyanate. The precipitation procedure was tested on a mixture of low molecular weight compounds (e.g. amino acids and non-amino organic acids) and it was shown that none of the tested compounds were precipitated.

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1007/s00216-016-9326-1

DO - 10.1007/s00216-016-9326-1

M3 - Journal article

C2 - 26894763

VL - 408

SP - 2303

EP - 2307

JO - Analusis

JF - Analusis

SN - 0365-4877

IS - 9

ER -

ID: 169136315