Activation of plant plasma membrane H+-ATPase by the non-ionic detergent Brij 58
Research output: Contribution to journal › Journal article › Research › peer-review
Plasma membrane vesicles were purified from tobacco callii and the modulation of H+-ATPase by detergents was investigated. The nonionic detergent Brij 58 not only activated ATP hydrolysis (2-fold) but also proton pumping (more than 4-fold). Triton X-100, within a more limited concentration range, produced a similar effect. The simultaneous activation of ATP hydrolysis and proton pumping is not compatible with current interpretations of effects of nonionic detergents on the H+-ATPase based on latency of the enzyme and opening of vesicles.
Original language | English |
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Journal | BBA - Biomembranes |
Volume | 1196 |
Issue number | 1 |
Pages (from-to) | 93-96 |
Number of pages | 4 |
ISSN | 0005-2736 |
DOIs | |
Publication status | Published - 23 Nov 1994 |
- ATPase, H-, Detergent activation, Plasma membrane, Tobacco calli
Research areas
ID: 245002558