Comparison of pharmacokinetic study profiles of insulin in rat plasma through conventional sampling and microsampling by micro-LC-MS/MS
Research output: Contribution to journal › Journal article › Research › peer-review
Documents
- Fulltext
Accepted author manuscript, 19.2 MB, PDF document
Aim: With microsamples of blood, full pharmacokinetic profiles from individual animals can be obtained as an alternative to the sparse-sampling approach, where conventional volume samples from several animals are required. However, microsamples require assays that are more sensitive. Methods: The sensitivity of the LC-MS assay was increased 47-fold using microflow LC-MS. Results & conclusion: By analyzing both microsamples and conventional samples from the same animals, it is demonstrated that sparse-sampling profiles can be nonrepresentative of the full profiles. This bias can affect the tested treatment by increasing or reducing its apparent effect. Microsampling enables unbiased results compared with sparse-sampling. An increase in assay sensitivity to balance the low sample volumes was achievable by microflow LC-MS.
Original language | English |
---|---|
Journal | Bioanalysis |
Volume | 15 |
Issue number | 5 |
Pages (from-to) | 283-294 |
Number of pages | 12 |
ISSN | 1757-6180 |
DOIs | |
Publication status | Published - 2023 |
- insulins, microflow liquid chromatography, microsampling, pharmacokinetics, DOPING CONTROL ANALYSIS, LIQUID-CHROMATOGRAPHY, MASS-SPECTROMETRY, WHOLE-BLOOD, PROTEINS, PEPTIDES, ANALOGS, QUANTIFICATION, URINE
Research areas
ID: 346597215