Comparison of pharmacokinetic study profiles of insulin in rat plasma through conventional sampling and microsampling by micro-LC-MS/MS

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Comparison of pharmacokinetic study profiles of insulin in rat plasma through conventional sampling and microsampling by micro-LC-MS/MS. / Troche, Gaudry Bruno; Søeborg, Tue; Bödvarsdottir, Thóra Brynja; Bjelke, Mads; Nielsen, Nikoline Juul.

In: Bioanalysis, Vol. 15, No. 5, 2023, p. 283-294.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Troche, GB, Søeborg, T, Bödvarsdottir, TB, Bjelke, M & Nielsen, NJ 2023, 'Comparison of pharmacokinetic study profiles of insulin in rat plasma through conventional sampling and microsampling by micro-LC-MS/MS', Bioanalysis, vol. 15, no. 5, pp. 283-294. https://doi.org/10.4155/bio-2023-0006

APA

Troche, G. B., Søeborg, T., Bödvarsdottir, T. B., Bjelke, M., & Nielsen, N. J. (2023). Comparison of pharmacokinetic study profiles of insulin in rat plasma through conventional sampling and microsampling by micro-LC-MS/MS. Bioanalysis, 15(5), 283-294. https://doi.org/10.4155/bio-2023-0006

Vancouver

Troche GB, Søeborg T, Bödvarsdottir TB, Bjelke M, Nielsen NJ. Comparison of pharmacokinetic study profiles of insulin in rat plasma through conventional sampling and microsampling by micro-LC-MS/MS. Bioanalysis. 2023;15(5):283-294. https://doi.org/10.4155/bio-2023-0006

Author

Troche, Gaudry Bruno ; Søeborg, Tue ; Bödvarsdottir, Thóra Brynja ; Bjelke, Mads ; Nielsen, Nikoline Juul. / Comparison of pharmacokinetic study profiles of insulin in rat plasma through conventional sampling and microsampling by micro-LC-MS/MS. In: Bioanalysis. 2023 ; Vol. 15, No. 5. pp. 283-294.

Bibtex

@article{9657981a77f24e1c80f18ca3826b726b,
title = "Comparison of pharmacokinetic study profiles of insulin in rat plasma through conventional sampling and microsampling by micro-LC-MS/MS",
abstract = "Aim: With microsamples of blood, full pharmacokinetic profiles from individual animals can be obtained as an alternative to the sparse-sampling approach, where conventional volume samples from several animals are required. However, microsamples require assays that are more sensitive. Methods: The sensitivity of the LC-MS assay was increased 47-fold using microflow LC-MS. Results & conclusion: By analyzing both microsamples and conventional samples from the same animals, it is demonstrated that sparse-sampling profiles can be nonrepresentative of the full profiles. This bias can affect the tested treatment by increasing or reducing its apparent effect. Microsampling enables unbiased results compared with sparse-sampling. An increase in assay sensitivity to balance the low sample volumes was achievable by microflow LC-MS.",
keywords = "insulins, microflow liquid chromatography, microsampling, pharmacokinetics, DOPING CONTROL ANALYSIS, LIQUID-CHROMATOGRAPHY, MASS-SPECTROMETRY, WHOLE-BLOOD, PROTEINS, PEPTIDES, ANALOGS, QUANTIFICATION, URINE",
author = "Troche, {Gaudry Bruno} and Tue S{\o}eborg and B{\"o}dvarsdottir, {Th{\'o}ra Brynja} and Mads Bjelke and Nielsen, {Nikoline Juul}",
year = "2023",
doi = "10.4155/bio-2023-0006",
language = "English",
volume = "15",
pages = "283--294",
journal = "Bioanalysis",
issn = "1757-6180",
publisher = "Future Science",
number = "5",

}

RIS

TY - JOUR

T1 - Comparison of pharmacokinetic study profiles of insulin in rat plasma through conventional sampling and microsampling by micro-LC-MS/MS

AU - Troche, Gaudry Bruno

AU - Søeborg, Tue

AU - Bödvarsdottir, Thóra Brynja

AU - Bjelke, Mads

AU - Nielsen, Nikoline Juul

PY - 2023

Y1 - 2023

N2 - Aim: With microsamples of blood, full pharmacokinetic profiles from individual animals can be obtained as an alternative to the sparse-sampling approach, where conventional volume samples from several animals are required. However, microsamples require assays that are more sensitive. Methods: The sensitivity of the LC-MS assay was increased 47-fold using microflow LC-MS. Results & conclusion: By analyzing both microsamples and conventional samples from the same animals, it is demonstrated that sparse-sampling profiles can be nonrepresentative of the full profiles. This bias can affect the tested treatment by increasing or reducing its apparent effect. Microsampling enables unbiased results compared with sparse-sampling. An increase in assay sensitivity to balance the low sample volumes was achievable by microflow LC-MS.

AB - Aim: With microsamples of blood, full pharmacokinetic profiles from individual animals can be obtained as an alternative to the sparse-sampling approach, where conventional volume samples from several animals are required. However, microsamples require assays that are more sensitive. Methods: The sensitivity of the LC-MS assay was increased 47-fold using microflow LC-MS. Results & conclusion: By analyzing both microsamples and conventional samples from the same animals, it is demonstrated that sparse-sampling profiles can be nonrepresentative of the full profiles. This bias can affect the tested treatment by increasing or reducing its apparent effect. Microsampling enables unbiased results compared with sparse-sampling. An increase in assay sensitivity to balance the low sample volumes was achievable by microflow LC-MS.

KW - insulins

KW - microflow liquid chromatography

KW - microsampling

KW - pharmacokinetics

KW - DOPING CONTROL ANALYSIS

KW - LIQUID-CHROMATOGRAPHY

KW - MASS-SPECTROMETRY

KW - WHOLE-BLOOD

KW - PROTEINS

KW - PEPTIDES

KW - ANALOGS

KW - QUANTIFICATION

KW - URINE

U2 - 10.4155/bio-2023-0006

DO - 10.4155/bio-2023-0006

M3 - Journal article

C2 - 37058314

VL - 15

SP - 283

EP - 294

JO - Bioanalysis

JF - Bioanalysis

SN - 1757-6180

IS - 5

ER -

ID: 346597215