Arabidopsis TWISTED DWARF1 functionally interacts with Auxin Exporter ABCB1 on the root plasma membrane
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Arabidopsis TWISTED DWARF1 functionally interacts with Auxin Exporter ABCB1 on the root plasma membrane. / Wang, Bangjun; Bailly, Aurélien; Zwiewka, Marta; Henrichs, Sina; Azzarello, Elisa; Mancuso, Stefano; Maeshima, Masayoshi; Friml, Jirí; Schulz, Alexander; Geisler, Markus.
In: Plant Cell, Vol. 25, No. 1, 2013, p. 202-214.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Arabidopsis TWISTED DWARF1 functionally interacts with Auxin Exporter ABCB1 on the root plasma membrane
AU - Wang, Bangjun
AU - Bailly, Aurélien
AU - Zwiewka, Marta
AU - Henrichs, Sina
AU - Azzarello, Elisa
AU - Mancuso, Stefano
AU - Maeshima, Masayoshi
AU - Friml, Jirí
AU - Schulz, Alexander
AU - Geisler, Markus
PY - 2013
Y1 - 2013
N2 - Plant architecture is influenced by the polar, cell-to-cell transport of auxin that is primarily provided and regulated by plasma membrane efflux catalysts of the PIN-FORMED and B family of ABC transporter (ABCB) classes. The latter were shown to require the functionality of the FK506 binding protein42 TWISTED DWARF1 (TWD1), although underlying mechanisms are unclear. By genetic manipulation of TWD1 expression, we show here that TWD1 affects shootward root auxin reflux and, thus, downstream developmental traits, such as epidermal twisting and gravitropism of the root. Using immunological assays, we demonstrate a predominant lateral, mainly outward-facing, plasma membrane location for TWD1 in the root epidermis characterized by the lateral marker ABC transporter G36/PLEIOTROPIC DRUG-RESISTANCE8/PENETRATION3. At these epidermal plasma membrane domains, TWD1 colocalizes with nonpolar ABCB1. In planta bioluminescence resonance energy transfer analysis was used to verify specific ABC transporter B1 (ABCB1)-TWD1 interaction. Our data support a model in which TWD1 promotes lateral ABCB-mediated auxin efflux via protein-protein interaction at the plasma membrane, minimizing reflux from the root apoplast into the cytoplasm.
AB - Plant architecture is influenced by the polar, cell-to-cell transport of auxin that is primarily provided and regulated by plasma membrane efflux catalysts of the PIN-FORMED and B family of ABC transporter (ABCB) classes. The latter were shown to require the functionality of the FK506 binding protein42 TWISTED DWARF1 (TWD1), although underlying mechanisms are unclear. By genetic manipulation of TWD1 expression, we show here that TWD1 affects shootward root auxin reflux and, thus, downstream developmental traits, such as epidermal twisting and gravitropism of the root. Using immunological assays, we demonstrate a predominant lateral, mainly outward-facing, plasma membrane location for TWD1 in the root epidermis characterized by the lateral marker ABC transporter G36/PLEIOTROPIC DRUG-RESISTANCE8/PENETRATION3. At these epidermal plasma membrane domains, TWD1 colocalizes with nonpolar ABCB1. In planta bioluminescence resonance energy transfer analysis was used to verify specific ABC transporter B1 (ABCB1)-TWD1 interaction. Our data support a model in which TWD1 promotes lateral ABCB-mediated auxin efflux via protein-protein interaction at the plasma membrane, minimizing reflux from the root apoplast into the cytoplasm.
KW - binding cassette transporter
KW - cells
KW - efflux
KW - immunophilin
KW - lateral root
KW - localization
KW - p-glycoproteins
KW - pin
KW - plant development
KW - resistance
U2 - 10.1105/tpc.112.105999
DO - 10.1105/tpc.112.105999
M3 - Journal article
C2 - 23321285
VL - 25
SP - 202
EP - 214
JO - The Plant Cell
JF - The Plant Cell
SN - 1040-4651
IS - 1
ER -
ID: 83913114