Expression of the sarcoplasmic reticulum Ca2+-ATPase in yeast
Research output: Contribution to journal › Journal article › Research › peer-review
We describe here an easy system for the production of mg amounts of the rabbit Ca2+-ATPase SERCA 1a in the yeast S. cerevisiae. The protein is present in several membranes, including the plasma membrane of the yeast, in a native conformation. It can be purified by immunoprecipitation and can be phosphorylated from ATP in a Ca2+-dependent manner. Using a temperature-sensitive secretion mutant strain, the fully active protein can also be obtained in secretory vesicles.
Original language | English |
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Journal | FEBS Letters |
Volume | 354 |
Issue number | 1 |
Pages (from-to) | 117-122 |
Number of pages | 6 |
ISSN | 0014-5793 |
DOIs | |
Publication status | Published - 31 Oct 1994 |
ID: 245002833