Arabidopsis ABCG28 is required for the apical accumulation of reactive oxygen species in growing pollen tubes
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Arabidopsis ABCG28 is required for the apical accumulation of reactive oxygen species in growing pollen tubes. / Do, Thanh Ha Thi; Choi, Hyunju; Palmgren, Michael; Martinoia, Enrico; Hwang, Jae Ung; Lee, Youngsook.
In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 116, No. 25, 18.06.2019, p. 12540-12549.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Arabidopsis ABCG28 is required for the apical accumulation of reactive oxygen species in growing pollen tubes
AU - Do, Thanh Ha Thi
AU - Choi, Hyunju
AU - Palmgren, Michael
AU - Martinoia, Enrico
AU - Hwang, Jae Ung
AU - Lee, Youngsook
PY - 2019/6/18
Y1 - 2019/6/18
N2 - Tip-focused accumulation of reactive oxygen species (ROS) is tightly associated with pollen tube growth and is thus critical for fertilization. However, it is unclear how tip-growing cells establish such specific ROS localization. Polyamines have been proposed to function in tip growth as precursors of the ROS, hydrogen peroxide. The ABC transporter AtABCG28 may regulate ROS status, as it contains multiple cysteine residues, a characteristic of proteins involved in ROS homeostasis. In this study, we found that AtABCG28 was specifically expressed in the mature pollen grains and pollen tubes. AtABCG28 was localized to secretory vesicles inside the pollen tube that moved toward and fused with the plasma membrane of the pollen tube tip. Knocking out AtABCG28 resulted in defective pollen tube growth, failure to localize polyamine and ROS to the growing pollen tube tip, and complete male sterility, whereas ectopic expression of this gene in root hair could recover ROS accumulation at the tip and improved the growth under high-pH conditions, which normally prevent ROS accumulation and tip growth. Together, these data suggest that AtABCG28 is critical for localizing polyamine and ROS at the growing tip. In addition, this function of AtABCG28 is likely to protect the pollen tube from the cytotoxicity of polyamine and contribute to the delivery of polyamine to the growing tip for incorporation into the expanding cell wall.
AB - Tip-focused accumulation of reactive oxygen species (ROS) is tightly associated with pollen tube growth and is thus critical for fertilization. However, it is unclear how tip-growing cells establish such specific ROS localization. Polyamines have been proposed to function in tip growth as precursors of the ROS, hydrogen peroxide. The ABC transporter AtABCG28 may regulate ROS status, as it contains multiple cysteine residues, a characteristic of proteins involved in ROS homeostasis. In this study, we found that AtABCG28 was specifically expressed in the mature pollen grains and pollen tubes. AtABCG28 was localized to secretory vesicles inside the pollen tube that moved toward and fused with the plasma membrane of the pollen tube tip. Knocking out AtABCG28 resulted in defective pollen tube growth, failure to localize polyamine and ROS to the growing pollen tube tip, and complete male sterility, whereas ectopic expression of this gene in root hair could recover ROS accumulation at the tip and improved the growth under high-pH conditions, which normally prevent ROS accumulation and tip growth. Together, these data suggest that AtABCG28 is critical for localizing polyamine and ROS at the growing tip. In addition, this function of AtABCG28 is likely to protect the pollen tube from the cytotoxicity of polyamine and contribute to the delivery of polyamine to the growing tip for incorporation into the expanding cell wall.
KW - ABC transporters
KW - AtABCG28
KW - Pollen tube growth
KW - Polyamine
KW - ROS
U2 - 10.1073/pnas.1902010116
DO - 10.1073/pnas.1902010116
M3 - Journal article
C2 - 31152136
AN - SCOPUS:85067605572
VL - 116
SP - 12540
EP - 12549
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
SN - 0027-8424
IS - 25
ER -
ID: 223678297