The lipid flippases ALA4 and ALA5 play critical roles in cell expansion and plant growth

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

The lipid flippases ALA4 and ALA5 play critical roles in cell expansion and plant growth. / Davis, Jeffrey A.; Pares, Randall B.; Bernstein, Tilde; McDowell, Stephen C.; Brown, Elizabeth; Stubrich, Jason; Rosenberg, Alexa; Cahoon, Edgar B.; Cahoon, Rebecca E.; Poulsen, Lisbeth R.; Palmgren, Michael; López-Marqués, Rosa L.; Harper, Jeffrey F.

In: Plant Physiology, Vol. 182, No. 4, 2020, p. 2111-2125.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Davis, JA, Pares, RB, Bernstein, T, McDowell, SC, Brown, E, Stubrich, J, Rosenberg, A, Cahoon, EB, Cahoon, RE, Poulsen, LR, Palmgren, M, López-Marqués, RL & Harper, JF 2020, 'The lipid flippases ALA4 and ALA5 play critical roles in cell expansion and plant growth', Plant Physiology, vol. 182, no. 4, pp. 2111-2125. https://doi.org/10.1104/PP.19.01332

APA

Davis, J. A., Pares, R. B., Bernstein, T., McDowell, S. C., Brown, E., Stubrich, J., Rosenberg, A., Cahoon, E. B., Cahoon, R. E., Poulsen, L. R., Palmgren, M., López-Marqués, R. L., & Harper, J. F. (2020). The lipid flippases ALA4 and ALA5 play critical roles in cell expansion and plant growth. Plant Physiology, 182(4), 2111-2125. https://doi.org/10.1104/PP.19.01332

Vancouver

Davis JA, Pares RB, Bernstein T, McDowell SC, Brown E, Stubrich J et al. The lipid flippases ALA4 and ALA5 play critical roles in cell expansion and plant growth. Plant Physiology. 2020;182(4):2111-2125. https://doi.org/10.1104/PP.19.01332

Author

Davis, Jeffrey A. ; Pares, Randall B. ; Bernstein, Tilde ; McDowell, Stephen C. ; Brown, Elizabeth ; Stubrich, Jason ; Rosenberg, Alexa ; Cahoon, Edgar B. ; Cahoon, Rebecca E. ; Poulsen, Lisbeth R. ; Palmgren, Michael ; López-Marqués, Rosa L. ; Harper, Jeffrey F. / The lipid flippases ALA4 and ALA5 play critical roles in cell expansion and plant growth. In: Plant Physiology. 2020 ; Vol. 182, No. 4. pp. 2111-2125.

Bibtex

@article{9378faf4dcd04a7fb8792e196cb9260b,
title = "The lipid flippases ALA4 and ALA5 play critical roles in cell expansion and plant growth",
abstract = "Aminophospholipid ATPases (ALAs) are lipid flippases involved in transporting specific lipids across membrane bilayers. Arabidopsis (Arabidopsis thaliana) contains 12 ALAs in five phylogenetic clusters, including four in cluster 3 (ALA4-ALA7). ALA4/5 and ALA6/7, are expressed primarily in vegetative tissues and pollen, respectively. Previously, a double knockout of ALA6/7 was shown to result in pollen fertility defects. Here we show that a double knockout of ALA4/5 results in dwarfism, characterized by reduced growth in rosettes (6.5-fold), roots (4.3-fold), bolts (4.5-fold), and hypocotyls (2-fold). Reduced cell size was observed for multiple vegetative cell types, suggesting a role for ALA4/5 in cellular expansion. Members of the third ALA cluster are at least partially interchangeable, as transgenes expressing ALA6 in vegetative tissues partially rescued ala4/5 mutant phenotypes, and expression of ALA4 transgenes in pollen fully rescued ala6/7 mutant fertility defects. ALA4-GFP displayed plasma membrane and endomembrane localization patterns when imaged in both guard cells and pollen. Lipid profiling revealed ala4/5 rosettes had perturbations in glycerolipid and sphingolipid content. Assays in yeast revealed that ALA5 can flip a variety of glycerolipids and the sphingolipid sphingomyelin across membranes. These results support a model whereby the flippase activity of ALA4 and ALA5 impacts the homeostasis of both glycerolipids and sphingolipids and is important for cellular expansion during vegetative growth.",
author = "Davis, {Jeffrey A.} and Pares, {Randall B.} and Tilde Bernstein and McDowell, {Stephen C.} and Elizabeth Brown and Jason Stubrich and Alexa Rosenberg and Cahoon, {Edgar B.} and Cahoon, {Rebecca E.} and Poulsen, {Lisbeth R.} and Michael Palmgren and L{\'o}pez-Marqu{\'e}s, {Rosa L.} and Harper, {Jeffrey F.}",
year = "2020",
doi = "10.1104/PP.19.01332",
language = "English",
volume = "182",
pages = "2111--2125",
journal = "Plant Physiology",
issn = "0032-0889",
publisher = "American Society of Plant Biologists",
number = "4",

}

RIS

TY - JOUR

T1 - The lipid flippases ALA4 and ALA5 play critical roles in cell expansion and plant growth

AU - Davis, Jeffrey A.

AU - Pares, Randall B.

AU - Bernstein, Tilde

AU - McDowell, Stephen C.

AU - Brown, Elizabeth

AU - Stubrich, Jason

AU - Rosenberg, Alexa

AU - Cahoon, Edgar B.

AU - Cahoon, Rebecca E.

AU - Poulsen, Lisbeth R.

AU - Palmgren, Michael

AU - López-Marqués, Rosa L.

AU - Harper, Jeffrey F.

PY - 2020

Y1 - 2020

N2 - Aminophospholipid ATPases (ALAs) are lipid flippases involved in transporting specific lipids across membrane bilayers. Arabidopsis (Arabidopsis thaliana) contains 12 ALAs in five phylogenetic clusters, including four in cluster 3 (ALA4-ALA7). ALA4/5 and ALA6/7, are expressed primarily in vegetative tissues and pollen, respectively. Previously, a double knockout of ALA6/7 was shown to result in pollen fertility defects. Here we show that a double knockout of ALA4/5 results in dwarfism, characterized by reduced growth in rosettes (6.5-fold), roots (4.3-fold), bolts (4.5-fold), and hypocotyls (2-fold). Reduced cell size was observed for multiple vegetative cell types, suggesting a role for ALA4/5 in cellular expansion. Members of the third ALA cluster are at least partially interchangeable, as transgenes expressing ALA6 in vegetative tissues partially rescued ala4/5 mutant phenotypes, and expression of ALA4 transgenes in pollen fully rescued ala6/7 mutant fertility defects. ALA4-GFP displayed plasma membrane and endomembrane localization patterns when imaged in both guard cells and pollen. Lipid profiling revealed ala4/5 rosettes had perturbations in glycerolipid and sphingolipid content. Assays in yeast revealed that ALA5 can flip a variety of glycerolipids and the sphingolipid sphingomyelin across membranes. These results support a model whereby the flippase activity of ALA4 and ALA5 impacts the homeostasis of both glycerolipids and sphingolipids and is important for cellular expansion during vegetative growth.

AB - Aminophospholipid ATPases (ALAs) are lipid flippases involved in transporting specific lipids across membrane bilayers. Arabidopsis (Arabidopsis thaliana) contains 12 ALAs in five phylogenetic clusters, including four in cluster 3 (ALA4-ALA7). ALA4/5 and ALA6/7, are expressed primarily in vegetative tissues and pollen, respectively. Previously, a double knockout of ALA6/7 was shown to result in pollen fertility defects. Here we show that a double knockout of ALA4/5 results in dwarfism, characterized by reduced growth in rosettes (6.5-fold), roots (4.3-fold), bolts (4.5-fold), and hypocotyls (2-fold). Reduced cell size was observed for multiple vegetative cell types, suggesting a role for ALA4/5 in cellular expansion. Members of the third ALA cluster are at least partially interchangeable, as transgenes expressing ALA6 in vegetative tissues partially rescued ala4/5 mutant phenotypes, and expression of ALA4 transgenes in pollen fully rescued ala6/7 mutant fertility defects. ALA4-GFP displayed plasma membrane and endomembrane localization patterns when imaged in both guard cells and pollen. Lipid profiling revealed ala4/5 rosettes had perturbations in glycerolipid and sphingolipid content. Assays in yeast revealed that ALA5 can flip a variety of glycerolipids and the sphingolipid sphingomyelin across membranes. These results support a model whereby the flippase activity of ALA4 and ALA5 impacts the homeostasis of both glycerolipids and sphingolipids and is important for cellular expansion during vegetative growth.

U2 - 10.1104/PP.19.01332

DO - 10.1104/PP.19.01332

M3 - Journal article

C2 - 32051180

AN - SCOPUS:85086172178

VL - 182

SP - 2111

EP - 2125

JO - Plant Physiology

JF - Plant Physiology

SN - 0032-0889

IS - 4

ER -

ID: 245000107