Imaging of Lipid Uptake in Arabidopsis Seedlings Utilizing Fluorescent Lipids and Confocal Microscopy

Research output: Contribution to journal › Journal article › Research › peer-review

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Imaging of Lipid Uptake in Arabidopsis Seedlings Utilizing Fluorescent Lipids and Confocal Microscopy. / López-Marqués, Rosa L.; Pomorski, Thomas G.

In: Bio-protocol, Vol. 11, No. 22, e4228, 2021.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

López-Marqués, RL & Pomorski, TG 2021, 'Imaging of Lipid Uptake in Arabidopsis Seedlings Utilizing Fluorescent Lipids and Confocal Microscopy', Bio-protocol, vol. 11, no. 22, e4228. https://doi.org/10.21769/BioProtoc.4228

APA

López-Marqués, R. L., & Pomorski, T. G. (2021). Imaging of Lipid Uptake in Arabidopsis Seedlings Utilizing Fluorescent Lipids and Confocal Microscopy. Bio-protocol, 11(22), [e4228]. https://doi.org/10.21769/BioProtoc.4228

Vancouver

López-Marqués RL, Pomorski TG. Imaging of Lipid Uptake in Arabidopsis Seedlings Utilizing Fluorescent Lipids and Confocal Microscopy. Bio-protocol. 2021;11(22). e4228. https://doi.org/10.21769/BioProtoc.4228

Author

López-Marqués, Rosa L. ; Pomorski, Thomas G. / Imaging of Lipid Uptake in Arabidopsis Seedlings Utilizing Fluorescent Lipids and Confocal Microscopy. In: Bio-protocol. 2021 ; Vol. 11, No. 22.

Bibtex

@article{c6f11835cfd94feebac66e45595c9c10,
title = "Imaging of Lipid Uptake in Arabidopsis Seedlings Utilizing Fluorescent Lipids and Confocal Microscopy",
abstract = "Eukaryotic cells use a diverse set of transporters to control the movement of lipids across their plasma membrane, which drastically affects membrane properties. Various tools and techniques to analyze the activity of these transporters have been developed. Among them, assays based on fluorescent phospholipid probes are particularly suitable, allowing for imaging and quantification of lipid internalization in living cells. Classically, these assays have been applied to yeast and animal cells. Here, we describe the adaptation of this powerful approach to characterize lipid internalization in plant roots and aerial tissues using confocal imaging.",
keywords = "Arabidopsis thaliana, Flippases, Guard cells, Leaves, Lipid transport, NBD-lipids, P4 ATPases, Roots",
author = "L{\'o}pez-Marqu{\'e}s, {Rosa L.} and Pomorski, {Thomas G.}",
note = "Publisher Copyright: {\textcopyright} 2021 The Authors.",
year = "2021",
doi = "10.21769/BioProtoc.4228",
language = "English",
volume = "11",
journal = "Bio-protocol",
issn = "2331-8325",
publisher = "bio-protocol",
number = "22",

}

RIS

TY - JOUR

T1 - Imaging of Lipid Uptake in Arabidopsis Seedlings Utilizing Fluorescent Lipids and Confocal Microscopy

AU - López-Marqués, Rosa L.

AU - Pomorski, Thomas G.

N1 - Publisher Copyright: © 2021 The Authors.

PY - 2021

Y1 - 2021

N2 - Eukaryotic cells use a diverse set of transporters to control the movement of lipids across their plasma membrane, which drastically affects membrane properties. Various tools and techniques to analyze the activity of these transporters have been developed. Among them, assays based on fluorescent phospholipid probes are particularly suitable, allowing for imaging and quantification of lipid internalization in living cells. Classically, these assays have been applied to yeast and animal cells. Here, we describe the adaptation of this powerful approach to characterize lipid internalization in plant roots and aerial tissues using confocal imaging.

AB - Eukaryotic cells use a diverse set of transporters to control the movement of lipids across their plasma membrane, which drastically affects membrane properties. Various tools and techniques to analyze the activity of these transporters have been developed. Among them, assays based on fluorescent phospholipid probes are particularly suitable, allowing for imaging and quantification of lipid internalization in living cells. Classically, these assays have been applied to yeast and animal cells. Here, we describe the adaptation of this powerful approach to characterize lipid internalization in plant roots and aerial tissues using confocal imaging.

KW - Arabidopsis thaliana

KW - Flippases

KW - Guard cells

KW - Leaves

KW - Lipid transport

KW - NBD-lipids

KW - P4 ATPases

KW - Roots

U2 - 10.21769/BioProtoc.4228

DO - 10.21769/BioProtoc.4228

M3 - Journal article

C2 - 34909449

AN - SCOPUS:85121982014

VL - 11

JO - Bio-protocol

JF - Bio-protocol

SN - 2331-8325

IS - 22

M1 - e4228

ER -

ID: 290601806