Temporal and Spatial Patterns of Zinc and Iron Accumulation during Barley (Hordeum vulgare L.) Grain Development
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Temporal and Spatial Patterns of Zinc and Iron Accumulation during Barley (Hordeum vulgare L.) Grain Development. / Detterbeck, Amelie; Pongrac, Paula; Persson, Daniel P.; Vogel-Mikus, Katarina; Kelemen, Mitja; Vavpetic, Primoz; Pelicon, Primoz; Arcon, Iztok; Husted, Søren; Schjoerring, Jan Kofod; Clemens, Stephan.
In: Journal of Agricultural and Food Chemistry, Vol. 68, No. 44, 2020, p. 12229-12240.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Temporal and Spatial Patterns of Zinc and Iron Accumulation during Barley (Hordeum vulgare L.) Grain Development
AU - Detterbeck, Amelie
AU - Pongrac, Paula
AU - Persson, Daniel P.
AU - Vogel-Mikus, Katarina
AU - Kelemen, Mitja
AU - Vavpetic, Primoz
AU - Pelicon, Primoz
AU - Arcon, Iztok
AU - Husted, Søren
AU - Schjoerring, Jan Kofod
AU - Clemens, Stephan
PY - 2020
Y1 - 2020
N2 - Breeding and engineering of biofortified crops will benefit from a better understanding of bottlenecks controlling micronutrient loading within the seeds. However, few studies have addressed the changes in micronutrient concentrations, localization, and speciation occurring over time. Therefore, we studied spatial patterns of zinc and iron accumulation during grain development in two barley lines with contrasting grain zinc concentrations. Microparticle-induced-X-ray emission and laser ablation-inductively coupled plasma mass spectrometry were used to determine tissue-specific accumulation of zinc, iron, phosphorus, and sulfur. Differences in zinc accumulation between the lines were most evident in the endosperm and aleurone. A gradual decrease in zinc concentrations from the aleurone to the underlying endosperm was observed, while iron and phosphorus concentrations decreased sharply. Iron co-localized with phosphorus in the aleurone, whereas zinc co-localized with sulfur in the sub-aleurone. We hypothesize that differences in grain zinc are largely explained by the endosperm storage capacity. Engineering attempts should be targeted accordingly.
AB - Breeding and engineering of biofortified crops will benefit from a better understanding of bottlenecks controlling micronutrient loading within the seeds. However, few studies have addressed the changes in micronutrient concentrations, localization, and speciation occurring over time. Therefore, we studied spatial patterns of zinc and iron accumulation during grain development in two barley lines with contrasting grain zinc concentrations. Microparticle-induced-X-ray emission and laser ablation-inductively coupled plasma mass spectrometry were used to determine tissue-specific accumulation of zinc, iron, phosphorus, and sulfur. Differences in zinc accumulation between the lines were most evident in the endosperm and aleurone. A gradual decrease in zinc concentrations from the aleurone to the underlying endosperm was observed, while iron and phosphorus concentrations decreased sharply. Iron co-localized with phosphorus in the aleurone, whereas zinc co-localized with sulfur in the sub-aleurone. We hypothesize that differences in grain zinc are largely explained by the endosperm storage capacity. Engineering attempts should be targeted accordingly.
KW - barley (Hordeum vulgare L.)
KW - biofortification
KW - grain development
KW - grain loading
KW - LA-ICP-MS
KW - mu-PIXE
U2 - 10.1021/acs.jafc.0c04833
DO - 10.1021/acs.jafc.0c04833
M3 - Journal article
C2 - 33070613
VL - 68
SP - 12229
EP - 12240
JO - Journal of Agricultural and Food Chemistry
JF - Journal of Agricultural and Food Chemistry
SN - 0021-8561
IS - 44
ER -
ID: 255460761