Suppression of XopQ–XopX-induced immune responses of rice by the type III effector XopG
Research output: Contribution to journal › Journal article › Research › peer-review
Standard
Suppression of XopQ–XopX-induced immune responses of rice by the type III effector XopG. / Deb, Sohini; Gokulan, C. G.; Nathawat, Rajkanwar; Patel, Hitendra K.; Sonti, Ramesh V.
In: Molecular Plant Pathology, Vol. 23, No. 5, 2022, p. 634-648.Research output: Contribution to journal › Journal article › Research › peer-review
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - Suppression of XopQ–XopX-induced immune responses of rice by the type III effector XopG
AU - Deb, Sohini
AU - Gokulan, C. G.
AU - Nathawat, Rajkanwar
AU - Patel, Hitendra K.
AU - Sonti, Ramesh V.
N1 - Publisher Copyright: © 2022 The Authors. Molecular Plant Pathology published by British Society for Plant Pathology and John Wiley & Sons Ltd.
PY - 2022
Y1 - 2022
N2 - Effectors that suppress effector-triggered immunity (ETI) are an essential part of the arms race in the co-evolution of bacterial pathogens and their host plants. Xanthomonas oryzae pv. oryzae uses multiple type III secretion system (T3SS) secreted effectors such as XopU, XopV, XopP, XopG, and AvrBs2 to suppress rice immune responses that are induced by the interaction of two other effectors, XopQ and XopX. Here we show that each of these five suppressors can interact individually with both XopQ and XopX. One of the suppressors, XopG, is a predicted metallopeptidase that appears to have been introduced into X. oryzae pv. oryzae by horizontal gene transfer. XopQ and XopX interact with each other in the nucleus while interaction with XopG sequesters them in the cytoplasm. The XopG E76A and XopG E85A mutants are defective in interaction with XopQ and XopX, and are also defective in suppression of XopQ–XopX-mediated immune responses. Both mutations individually affect the virulence-promoting ability of XopG. These results indicate that XopG is important for X. oryzae pv. oryzae virulence and provide insights into the mechanisms by which this protein suppresses ETI in rice.
AB - Effectors that suppress effector-triggered immunity (ETI) are an essential part of the arms race in the co-evolution of bacterial pathogens and their host plants. Xanthomonas oryzae pv. oryzae uses multiple type III secretion system (T3SS) secreted effectors such as XopU, XopV, XopP, XopG, and AvrBs2 to suppress rice immune responses that are induced by the interaction of two other effectors, XopQ and XopX. Here we show that each of these five suppressors can interact individually with both XopQ and XopX. One of the suppressors, XopG, is a predicted metallopeptidase that appears to have been introduced into X. oryzae pv. oryzae by horizontal gene transfer. XopQ and XopX interact with each other in the nucleus while interaction with XopG sequesters them in the cytoplasm. The XopG E76A and XopG E85A mutants are defective in interaction with XopQ and XopX, and are also defective in suppression of XopQ–XopX-mediated immune responses. Both mutations individually affect the virulence-promoting ability of XopG. These results indicate that XopG is important for X. oryzae pv. oryzae virulence and provide insights into the mechanisms by which this protein suppresses ETI in rice.
KW - effector
KW - effector-triggered immunity
KW - resistance
KW - rice
KW - Xanthomonas oryzae pv. oryzae
KW - XopG
KW - XopQ
KW - XopX
U2 - 10.1111/mpp.13184
DO - 10.1111/mpp.13184
M3 - Journal article
C2 - 35150038
AN - SCOPUS:85124503323
VL - 23
SP - 634
EP - 648
JO - Molecular Plant Pathology
JF - Molecular Plant Pathology
SN - 1464-6722
IS - 5
ER -
ID: 298998868