Seronegative patients with primary Sjögren's syndrome and non-pSS sicca test positive for anti-SSA/Ro52 and -Ro60 in saliva

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Seronegative patients with primary Sjögren's syndrome and non-pSS sicca test positive for anti-SSA/Ro52 and -Ro60 in saliva. / Kamounah, Sarah; Wei, Fang; Park, Jin Kyun; Song, Yeong-Wook; Chia, David; Wong, David T W; Pedersen, Anne Marie Lynge.

In: B B A - Molecular Basis of Disease, 04.2024, p. 167168.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Kamounah, S, Wei, F, Park, JK, Song, Y-W, Chia, D, Wong, DTW & Pedersen, AML 2024, 'Seronegative patients with primary Sjögren's syndrome and non-pSS sicca test positive for anti-SSA/Ro52 and -Ro60 in saliva', B B A - Molecular Basis of Disease, pp. 167168. https://doi.org/10.1016/j.bbadis.2024.167168

APA

Kamounah, S., Wei, F., Park, J. K., Song, Y-W., Chia, D., Wong, D. T. W., & Pedersen, A. M. L. (2024). Seronegative patients with primary Sjögren's syndrome and non-pSS sicca test positive for anti-SSA/Ro52 and -Ro60 in saliva. B B A - Molecular Basis of Disease, 167168. https://doi.org/10.1016/j.bbadis.2024.167168

Vancouver

Kamounah S, Wei F, Park JK, Song Y-W, Chia D, Wong DTW et al. Seronegative patients with primary Sjögren's syndrome and non-pSS sicca test positive for anti-SSA/Ro52 and -Ro60 in saliva. B B A - Molecular Basis of Disease. 2024 Apr;167168. https://doi.org/10.1016/j.bbadis.2024.167168

Author

Kamounah, Sarah ; Wei, Fang ; Park, Jin Kyun ; Song, Yeong-Wook ; Chia, David ; Wong, David T W ; Pedersen, Anne Marie Lynge. / Seronegative patients with primary Sjögren's syndrome and non-pSS sicca test positive for anti-SSA/Ro52 and -Ro60 in saliva. In: B B A - Molecular Basis of Disease. 2024 ; pp. 167168.

Bibtex

@article{c2bd4bb3ddf349c5a31b5e43b19202fc,
title = "Seronegative patients with primary Sj{\"o}gren's syndrome and non-pSS sicca test positive for anti-SSA/Ro52 and -Ro60 in saliva",
abstract = "OBJECTIVES: Testing for anti-SSA/Ro antibodies in serum is essential in the diagnostic work-up for primary Sj{\"o}gren's syndrome (pSS). In this study, we aimed to validate our previous assay for detection of salivary anti-SSA/Ro52, and to develop assays for detection of salivary anti-SSA/Ro60 and for detection of anti-Ro52 and -Ro60 in plasma using the electric field-induced release and measurement (EFIRM) platform.METHODS: Whole saliva samples from two independent Danish cohorts (DN1 and DN2) including 49 patients with pSS, 73 patients with sicca symptoms, but not fulfilling the classification criteria for pSS (non-pSS sicca), and 51 healthy controls (HC), as well as plasma samples from the DN1 cohort were analyzed using EFIRM to detect anti-SSA/Ro52 and -Ro60.RESULTS: In the DN1 cohort, 100 % in the pSS group and 16 % in the non-pSS sicca group were serum anti-SSA/Ro positive by ELISA. EFIRM detected anti-SSA (Ro52 and/or -Ro60) in plasma and saliva in 100 % and 96 % patients with pSS, and 16 % and 29 % with non-pSS sicca. In the DN2 cohort, 80 % patients with pSS and 26 % with non-pSS sicca were serum anti-SSA/Ro positive. Salivary anti-SSA discriminated patients with pSS from HC and non-pSS sicca with an AUC range of 0.74-0.96 in the DN1 and DN2 cohorts. EFIRM discriminated pSS from non-pSS sicca with an AUC of 0.98 in plasma.CONCLUSION: Our findings suggest that salivary anti-SSA/Ro antibodies are potential discriminatory biomarkers for pSS, which may also identify seronegative patients, addressing the unmet clinical need of early detection and stratification of pSS.",
author = "Sarah Kamounah and Fang Wei and Park, {Jin Kyun} and Yeong-Wook Song and David Chia and Wong, {David T W} and Pedersen, {Anne Marie Lynge}",
note = "Copyright {\textcopyright} 2024. Published by Elsevier B.V.",
year = "2024",
month = apr,
doi = "10.1016/j.bbadis.2024.167168",
language = "English",
pages = "167168",
journal = "B B A - Molecular Basis of Disease",
issn = "0925-4439",
publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - Seronegative patients with primary Sjögren's syndrome and non-pSS sicca test positive for anti-SSA/Ro52 and -Ro60 in saliva

AU - Kamounah, Sarah

AU - Wei, Fang

AU - Park, Jin Kyun

AU - Song, Yeong-Wook

AU - Chia, David

AU - Wong, David T W

AU - Pedersen, Anne Marie Lynge

N1 - Copyright © 2024. Published by Elsevier B.V.

PY - 2024/4

Y1 - 2024/4

N2 - OBJECTIVES: Testing for anti-SSA/Ro antibodies in serum is essential in the diagnostic work-up for primary Sjögren's syndrome (pSS). In this study, we aimed to validate our previous assay for detection of salivary anti-SSA/Ro52, and to develop assays for detection of salivary anti-SSA/Ro60 and for detection of anti-Ro52 and -Ro60 in plasma using the electric field-induced release and measurement (EFIRM) platform.METHODS: Whole saliva samples from two independent Danish cohorts (DN1 and DN2) including 49 patients with pSS, 73 patients with sicca symptoms, but not fulfilling the classification criteria for pSS (non-pSS sicca), and 51 healthy controls (HC), as well as plasma samples from the DN1 cohort were analyzed using EFIRM to detect anti-SSA/Ro52 and -Ro60.RESULTS: In the DN1 cohort, 100 % in the pSS group and 16 % in the non-pSS sicca group were serum anti-SSA/Ro positive by ELISA. EFIRM detected anti-SSA (Ro52 and/or -Ro60) in plasma and saliva in 100 % and 96 % patients with pSS, and 16 % and 29 % with non-pSS sicca. In the DN2 cohort, 80 % patients with pSS and 26 % with non-pSS sicca were serum anti-SSA/Ro positive. Salivary anti-SSA discriminated patients with pSS from HC and non-pSS sicca with an AUC range of 0.74-0.96 in the DN1 and DN2 cohorts. EFIRM discriminated pSS from non-pSS sicca with an AUC of 0.98 in plasma.CONCLUSION: Our findings suggest that salivary anti-SSA/Ro antibodies are potential discriminatory biomarkers for pSS, which may also identify seronegative patients, addressing the unmet clinical need of early detection and stratification of pSS.

AB - OBJECTIVES: Testing for anti-SSA/Ro antibodies in serum is essential in the diagnostic work-up for primary Sjögren's syndrome (pSS). In this study, we aimed to validate our previous assay for detection of salivary anti-SSA/Ro52, and to develop assays for detection of salivary anti-SSA/Ro60 and for detection of anti-Ro52 and -Ro60 in plasma using the electric field-induced release and measurement (EFIRM) platform.METHODS: Whole saliva samples from two independent Danish cohorts (DN1 and DN2) including 49 patients with pSS, 73 patients with sicca symptoms, but not fulfilling the classification criteria for pSS (non-pSS sicca), and 51 healthy controls (HC), as well as plasma samples from the DN1 cohort were analyzed using EFIRM to detect anti-SSA/Ro52 and -Ro60.RESULTS: In the DN1 cohort, 100 % in the pSS group and 16 % in the non-pSS sicca group were serum anti-SSA/Ro positive by ELISA. EFIRM detected anti-SSA (Ro52 and/or -Ro60) in plasma and saliva in 100 % and 96 % patients with pSS, and 16 % and 29 % with non-pSS sicca. In the DN2 cohort, 80 % patients with pSS and 26 % with non-pSS sicca were serum anti-SSA/Ro positive. Salivary anti-SSA discriminated patients with pSS from HC and non-pSS sicca with an AUC range of 0.74-0.96 in the DN1 and DN2 cohorts. EFIRM discriminated pSS from non-pSS sicca with an AUC of 0.98 in plasma.CONCLUSION: Our findings suggest that salivary anti-SSA/Ro antibodies are potential discriminatory biomarkers for pSS, which may also identify seronegative patients, addressing the unmet clinical need of early detection and stratification of pSS.

U2 - 10.1016/j.bbadis.2024.167168

DO - 10.1016/j.bbadis.2024.167168

M3 - Journal article

C2 - 38641012

SP - 167168

JO - B B A - Molecular Basis of Disease

JF - B B A - Molecular Basis of Disease

SN - 0925-4439

ER -

ID: 389311799