Inactivation of Cryptosporidium parvum oocysts and faecal indicator bacteria in cattle slurry by addition of ammonia

Research output: Contribution to journal › Journal article › Research › peer-review

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Inactivation of Cryptosporidium parvum oocysts and faecal indicator bacteria in cattle slurry by addition of ammonia. / Petersen, H. H.; Dalsgaard, A.; Vinneras, B.; Jensen, L. S.; Le, T. T.A.; Petersen, M. A.; Enemark, H. L.; Forslund, A.

In: Journal of Applied Microbiology, Vol. 130, No. 5, 2021, p. 1745-1757.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Petersen, HH, Dalsgaard, A, Vinneras, B, Jensen, LS, Le, TTA, Petersen, MA, Enemark, HL & Forslund, A 2021, 'Inactivation of Cryptosporidium parvum oocysts and faecal indicator bacteria in cattle slurry by addition of ammonia', Journal of Applied Microbiology, vol. 130, no. 5, pp. 1745-1757. https://doi.org/10.1111/jam.14881

APA

Petersen, H. H., Dalsgaard, A., Vinneras, B., Jensen, L. S., Le, T. T. A., Petersen, M. A., Enemark, H. L., & Forslund, A. (2021). Inactivation of Cryptosporidium parvum oocysts and faecal indicator bacteria in cattle slurry by addition of ammonia. Journal of Applied Microbiology, 130(5), 1745-1757. https://doi.org/10.1111/jam.14881

Vancouver

Petersen HH, Dalsgaard A, Vinneras B, Jensen LS, Le TTA, Petersen MA et al. Inactivation of Cryptosporidium parvum oocysts and faecal indicator bacteria in cattle slurry by addition of ammonia. Journal of Applied Microbiology. 2021;130(5):1745-1757. https://doi.org/10.1111/jam.14881

Author

Petersen, H. H. ; Dalsgaard, A. ; Vinneras, B. ; Jensen, L. S. ; Le, T. T.A. ; Petersen, M. A. ; Enemark, H. L. ; Forslund, A. / Inactivation of Cryptosporidium parvum oocysts and faecal indicator bacteria in cattle slurry by addition of ammonia. In: Journal of Applied Microbiology. 2021 ; Vol. 130, No. 5. pp. 1745-1757.

Bibtex

@article{2c0f422b64cc43709d2135149fc61e0a,
title = "Inactivation of Cryptosporidium parvum oocysts and faecal indicator bacteria in cattle slurry by addition of ammonia",
abstract = "Aims: To determine inactivation of Cryptosporidium parvum oocysts and reduction of Escherichia coli and enterococci in cattle slurry added aqueous ammonia. Methods and Results: Escherichia coli, enterococci and nonviable C. parvum oocysts (DAPI+PI+) were enumerated every second day for 2 weeks in cattle slurry amended with 60 mmol l−1 aq. ammonia and compared with untreated slurry at three temperatures. Regardless of temperature, the proportion of nonviable C. parvum oocysts increased significantly faster over time in slurry with added ammonia than raw slurry (P = 0·021) corresponding to 62·0% higher inactivation (P = 0·001) at day 14. Additionally, 91·8% fewer E. coli and 27·3% fewer enterococci were observed in slurry added ammonia at day 14 compared to raw slurry. Conclusion: The addition of aqueous ammonia to raw slurry significantly reduced the viability of C. parvum oocysts and numbers of bacterial indicators. Hence, ammonia is usable at lower pathogen concentrations in slurry before application to agricultural land. Significance and Impact of the Study: Livestock waste is a valuable source of plant nutrients and organic matter, but may contain high concentrations of pathogens like E. coli and Cryptosporidium sp. that can be spread in the environment, and cause disease outbreaks. However, die-off rates of pathogens in organic waste can increase following increasing ammonia concentrations.",
keywords = "ammonia, cattle slurry, Cryptosporidium parvum, enterococci, Escherichia coli, faecal indicator bacteria",
author = "Petersen, {H. H.} and A. Dalsgaard and B. Vinneras and Jensen, {L. S.} and Le, {T. T.A.} and Petersen, {M. A.} and Enemark, {H. L.} and A. Forslund",
year = "2021",
doi = "10.1111/jam.14881",
language = "English",
volume = "130",
pages = "1745--1757",
journal = "Proceedings of the Society for Applied Bacteriology",
issn = "0266-8254",
publisher = "Wiley-Blackwell",
number = "5",

}

RIS

TY - JOUR

T1 - Inactivation of Cryptosporidium parvum oocysts and faecal indicator bacteria in cattle slurry by addition of ammonia

AU - Petersen, H. H.

AU - Dalsgaard, A.

AU - Vinneras, B.

AU - Jensen, L. S.

AU - Le, T. T.A.

AU - Petersen, M. A.

AU - Enemark, H. L.

AU - Forslund, A.

PY - 2021

Y1 - 2021

N2 - Aims: To determine inactivation of Cryptosporidium parvum oocysts and reduction of Escherichia coli and enterococci in cattle slurry added aqueous ammonia. Methods and Results: Escherichia coli, enterococci and nonviable C. parvum oocysts (DAPI+PI+) were enumerated every second day for 2 weeks in cattle slurry amended with 60 mmol l−1 aq. ammonia and compared with untreated slurry at three temperatures. Regardless of temperature, the proportion of nonviable C. parvum oocysts increased significantly faster over time in slurry with added ammonia than raw slurry (P = 0·021) corresponding to 62·0% higher inactivation (P = 0·001) at day 14. Additionally, 91·8% fewer E. coli and 27·3% fewer enterococci were observed in slurry added ammonia at day 14 compared to raw slurry. Conclusion: The addition of aqueous ammonia to raw slurry significantly reduced the viability of C. parvum oocysts and numbers of bacterial indicators. Hence, ammonia is usable at lower pathogen concentrations in slurry before application to agricultural land. Significance and Impact of the Study: Livestock waste is a valuable source of plant nutrients and organic matter, but may contain high concentrations of pathogens like E. coli and Cryptosporidium sp. that can be spread in the environment, and cause disease outbreaks. However, die-off rates of pathogens in organic waste can increase following increasing ammonia concentrations.

AB - Aims: To determine inactivation of Cryptosporidium parvum oocysts and reduction of Escherichia coli and enterococci in cattle slurry added aqueous ammonia. Methods and Results: Escherichia coli, enterococci and nonviable C. parvum oocysts (DAPI+PI+) were enumerated every second day for 2 weeks in cattle slurry amended with 60 mmol l−1 aq. ammonia and compared with untreated slurry at three temperatures. Regardless of temperature, the proportion of nonviable C. parvum oocysts increased significantly faster over time in slurry with added ammonia than raw slurry (P = 0·021) corresponding to 62·0% higher inactivation (P = 0·001) at day 14. Additionally, 91·8% fewer E. coli and 27·3% fewer enterococci were observed in slurry added ammonia at day 14 compared to raw slurry. Conclusion: The addition of aqueous ammonia to raw slurry significantly reduced the viability of C. parvum oocysts and numbers of bacterial indicators. Hence, ammonia is usable at lower pathogen concentrations in slurry before application to agricultural land. Significance and Impact of the Study: Livestock waste is a valuable source of plant nutrients and organic matter, but may contain high concentrations of pathogens like E. coli and Cryptosporidium sp. that can be spread in the environment, and cause disease outbreaks. However, die-off rates of pathogens in organic waste can increase following increasing ammonia concentrations.

KW - ammonia

KW - cattle slurry

KW - Cryptosporidium parvum

KW - enterococci

KW - Escherichia coli

KW - faecal indicator bacteria

U2 - 10.1111/jam.14881

DO - 10.1111/jam.14881

M3 - Journal article

C2 - 33012074

AN - SCOPUS:85092939565

VL - 130

SP - 1745

EP - 1757

JO - Proceedings of the Society for Applied Bacteriology

JF - Proceedings of the Society for Applied Bacteriology

SN - 0266-8254

IS - 5

ER -

ID: 250828344