Improvement of Tryptophan Analysis by Liquid Chromatography-Single Quadrupole Mass Spectrometry Through the Evaluation of Multiple Parameters

Research output: Contribution to journalJournal articleResearchpeer-review

Standard

Improvement of Tryptophan Analysis by Liquid Chromatography-Single Quadrupole Mass Spectrometry Through the Evaluation of Multiple Parameters. / la Cour, Rasmus; Jørgensen, Henning; Schjoerring, Jan K.

In: Frontiers in Chemistry, Vol. 7, 797, 19.11.2019, p. 1-7.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

la Cour, R, Jørgensen, H & Schjoerring, JK 2019, 'Improvement of Tryptophan Analysis by Liquid Chromatography-Single Quadrupole Mass Spectrometry Through the Evaluation of Multiple Parameters', Frontiers in Chemistry, vol. 7, 797, pp. 1-7. https://doi.org/10.3389/fchem.2019.00797

APA

la Cour, R., Jørgensen, H., & Schjoerring, J. K. (2019). Improvement of Tryptophan Analysis by Liquid Chromatography-Single Quadrupole Mass Spectrometry Through the Evaluation of Multiple Parameters. Frontiers in Chemistry, 7, 1-7. [797]. https://doi.org/10.3389/fchem.2019.00797

Vancouver

la Cour R, Jørgensen H, Schjoerring JK. Improvement of Tryptophan Analysis by Liquid Chromatography-Single Quadrupole Mass Spectrometry Through the Evaluation of Multiple Parameters. Frontiers in Chemistry. 2019 Nov 19;7:1-7. 797. https://doi.org/10.3389/fchem.2019.00797

Author

la Cour, Rasmus ; Jørgensen, Henning ; Schjoerring, Jan K. / Improvement of Tryptophan Analysis by Liquid Chromatography-Single Quadrupole Mass Spectrometry Through the Evaluation of Multiple Parameters. In: Frontiers in Chemistry. 2019 ; Vol. 7. pp. 1-7.

Bibtex

@article{38b6e2cfeed649f092cb9046e5538104,
title = "Improvement of Tryptophan Analysis by Liquid Chromatography-Single Quadrupole Mass Spectrometry Through the Evaluation of Multiple Parameters",
abstract = "Tryptophan is a key component in many biological processes and an essential amino acid in food and feed materials. Analysis of the tryptophan content in proteins or protein-containing matrices has always been a challenge. We show here that the preparation of samples prior to tryptophan analysis can be significantly simplified, and the time consumption reduced, by using ascorbic acid as antioxidant to eliminate the problem of tryptophan degradation during alkaline hydrolysis. Combined with separation by HPLC and detection by Single Quadrupole Mass Spectrometry, this allows the analytical run time to be reduced to 10 min. The alkaline hydrolysate obtained in the method presented here may be combined with the oxidized hydrolysate obtained when sulfur-containing amino acids are to be measured, thus essentially providing two analyses for the time of one.",
keywords = "amino acid profile, high-throughput, HPLC, LC-MS, protein",
author = "{la Cour}, Rasmus and Henning J{\o}rgensen and Schjoerring, {Jan K.}",
year = "2019",
month = "11",
day = "19",
doi = "10.3389/fchem.2019.00797",
language = "English",
volume = "7",
pages = "1--7",
journal = "Frontiers in Chemistry",
issn = "2296-2646",
publisher = "Frontiers Media S.A.",

}

RIS

TY - JOUR

T1 - Improvement of Tryptophan Analysis by Liquid Chromatography-Single Quadrupole Mass Spectrometry Through the Evaluation of Multiple Parameters

AU - la Cour, Rasmus

AU - Jørgensen, Henning

AU - Schjoerring, Jan K.

PY - 2019/11/19

Y1 - 2019/11/19

N2 - Tryptophan is a key component in many biological processes and an essential amino acid in food and feed materials. Analysis of the tryptophan content in proteins or protein-containing matrices has always been a challenge. We show here that the preparation of samples prior to tryptophan analysis can be significantly simplified, and the time consumption reduced, by using ascorbic acid as antioxidant to eliminate the problem of tryptophan degradation during alkaline hydrolysis. Combined with separation by HPLC and detection by Single Quadrupole Mass Spectrometry, this allows the analytical run time to be reduced to 10 min. The alkaline hydrolysate obtained in the method presented here may be combined with the oxidized hydrolysate obtained when sulfur-containing amino acids are to be measured, thus essentially providing two analyses for the time of one.

AB - Tryptophan is a key component in many biological processes and an essential amino acid in food and feed materials. Analysis of the tryptophan content in proteins or protein-containing matrices has always been a challenge. We show here that the preparation of samples prior to tryptophan analysis can be significantly simplified, and the time consumption reduced, by using ascorbic acid as antioxidant to eliminate the problem of tryptophan degradation during alkaline hydrolysis. Combined with separation by HPLC and detection by Single Quadrupole Mass Spectrometry, this allows the analytical run time to be reduced to 10 min. The alkaline hydrolysate obtained in the method presented here may be combined with the oxidized hydrolysate obtained when sulfur-containing amino acids are to be measured, thus essentially providing two analyses for the time of one.

KW - amino acid profile

KW - high-throughput

KW - HPLC

KW - LC-MS

KW - protein

U2 - 10.3389/fchem.2019.00797

DO - 10.3389/fchem.2019.00797

M3 - Journal article

C2 - 31803725

AN - SCOPUS:85076687243

VL - 7

SP - 1

EP - 7

JO - Frontiers in Chemistry

JF - Frontiers in Chemistry

SN - 2296-2646

M1 - 797

ER -

ID: 234079453