Genome and chromosome identification in cultivated barley and related species of the Triticeae (Poaceae) by in situ hybridization with the GAA-satellite sequence
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Genome and chromosome identification in cultivated barley and related species of the Triticeae (Poaceae) by in situ hybridization with the GAA-satellite sequence. / Pedersen, C.; Rasmussen, S. K.; Linde-Laursen, I.
In: Genome, Vol. 39, No. 1, 01.01.1996, p. 93-104.Research output: Contribution to journal › Journal article › Research › peer-review
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T1 - Genome and chromosome identification in cultivated barley and related species of the Triticeae (Poaceae) by in situ hybridization with the GAA-satellite sequence
AU - Pedersen, C.
AU - Rasmussen, S. K.
AU - Linde-Laursen, I.
PY - 1996/1/1
Y1 - 1996/1/1
N2 - The satellite sequence studied was primarily composed of GAA repeats organized in long tracts of heterochromatic DNA. Fluorescent in situ hybridization (FISH) with the GAA satellite (GAA banding) to the chromosomes of barley, wheat, rye, and other Triticeae species produced banding patterns similar to those obtained by N-banding. The GAA-banding patterns of barley are described in detail and those of 12 other Triticeae species are described briefly. In situ hybridization with the GAA-satellite sequence permits identification of all the chromosomes of barley. It is a valuable alternative to other banding techniques, especially in connection with physical gene mapping by FISH. The application of the GAA-satellite sequence for the characterization of genomes in phylogenetic studies of genera containing the sequence is discussed.
AB - The satellite sequence studied was primarily composed of GAA repeats organized in long tracts of heterochromatic DNA. Fluorescent in situ hybridization (FISH) with the GAA satellite (GAA banding) to the chromosomes of barley, wheat, rye, and other Triticeae species produced banding patterns similar to those obtained by N-banding. The GAA-banding patterns of barley are described in detail and those of 12 other Triticeae species are described briefly. In situ hybridization with the GAA-satellite sequence permits identification of all the chromosomes of barley. It is a valuable alternative to other banding techniques, especially in connection with physical gene mapping by FISH. The application of the GAA-satellite sequence for the characterization of genomes in phylogenetic studies of genera containing the sequence is discussed.
KW - Chromosome identification
KW - GAA-satellite sequence
KW - Genome differentiation
KW - Hordeum vulgare
KW - Triticeae
UR - http://www.scopus.com/inward/record.url?scp=0030087749&partnerID=8YFLogxK
U2 - 10.1139/g96-013
DO - 10.1139/g96-013
M3 - Journal article
C2 - 8851798
AN - SCOPUS:0030087749
VL - 39
SP - 93
EP - 104
JO - Genome
JF - Genome
SN - 0831-2796
IS - 1
ER -
ID: 204470143