Biocontrol effect of Clonostachys rosea on Fusarium graminearum infection and mycotoxin detoxification in oat (Avena sativa)
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Biocontrol effect of Clonostachys rosea on Fusarium graminearum infection and mycotoxin detoxification in oat (Avena sativa). / Khairullina, Alfia; Micic, Nikola; Jørgensen, Hans J.Lyngs; Bjarnholt, Nanna; Bülow, Leif; Collinge, David B.; Jensen, Birgit.
In: Plants, Vol. 12, No. 3, 500, 2023.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Biocontrol effect of Clonostachys rosea on Fusarium graminearum infection and mycotoxin detoxification in oat (Avena sativa)
AU - Khairullina, Alfia
AU - Micic, Nikola
AU - Jørgensen, Hans J.Lyngs
AU - Bjarnholt, Nanna
AU - Bülow, Leif
AU - Collinge, David B.
AU - Jensen, Birgit
N1 - Publisher Copyright: © 2023 by the authors.
PY - 2023
Y1 - 2023
N2 - Oat (Avena sativa) is susceptible to Fusarium head blight (FHB). The quality of oat grain is threatened by the accumulation of mycotoxins, particularly the trichothecene deoxynivalenol (DON), which also acts as a virulence factor for the main pathogen Fusarium graminearum. The plant can defend itself, e.g., by DON detoxification by UGT-glycosyltransferases (UTGs) and accumulation of PR-proteins, even though these mechanisms do not deliver effective levels of resistance. We studied the ability of the fungal biocontrol agent (BCA) Clonostachys rosea to reduce FHB and mycotoxin accumulation. Greenhouse trials showed that C. rosea-inoculation of oat spikelets at anthesis 3 days prior to F. graminearum inoculation reduced both the amount of Fusarium DNA (79%) and DON level (80%) in mature oat kernels substantially. DON applied to C. rosea-treated spikelets resulted in higher conversion of DON to DON-3-Glc than in mock treated plants. Moreover, there was a significant enhancement of expression of two oat UGT-glycosyltransferase genes in C. rosea-treated oat. In addition, C. rosea treatment activated expression of genes encoding four PR-proteins and a WRKY23-like transcription factor, suggesting that C. rosea may induce resistance in oat. Thus, C. rosea IK726 has strong potential to be used as a BCA against FHB in oat as it inhibits F. graminearum infection effectively, whilst detoxifying DON mycotoxin rapidly.
AB - Oat (Avena sativa) is susceptible to Fusarium head blight (FHB). The quality of oat grain is threatened by the accumulation of mycotoxins, particularly the trichothecene deoxynivalenol (DON), which also acts as a virulence factor for the main pathogen Fusarium graminearum. The plant can defend itself, e.g., by DON detoxification by UGT-glycosyltransferases (UTGs) and accumulation of PR-proteins, even though these mechanisms do not deliver effective levels of resistance. We studied the ability of the fungal biocontrol agent (BCA) Clonostachys rosea to reduce FHB and mycotoxin accumulation. Greenhouse trials showed that C. rosea-inoculation of oat spikelets at anthesis 3 days prior to F. graminearum inoculation reduced both the amount of Fusarium DNA (79%) and DON level (80%) in mature oat kernels substantially. DON applied to C. rosea-treated spikelets resulted in higher conversion of DON to DON-3-Glc than in mock treated plants. Moreover, there was a significant enhancement of expression of two oat UGT-glycosyltransferase genes in C. rosea-treated oat. In addition, C. rosea treatment activated expression of genes encoding four PR-proteins and a WRKY23-like transcription factor, suggesting that C. rosea may induce resistance in oat. Thus, C. rosea IK726 has strong potential to be used as a BCA against FHB in oat as it inhibits F. graminearum infection effectively, whilst detoxifying DON mycotoxin rapidly.
KW - biocontrol
KW - Clonostachys rosea
KW - deoxynivalenol
KW - Fusarium head blight
KW - mycotoxins
KW - oat
U2 - 10.3390/plants12030500
DO - 10.3390/plants12030500
M3 - Journal article
C2 - 36771583
AN - SCOPUS:85147827627
VL - 12
JO - Plants
JF - Plants
SN - 2223-7747
IS - 3
M1 - 500
ER -
ID: 337439599