Synthesis of 11-carbon terpenoids in yeast using protein and metabolic engineering

Research output: Contribution to journal › Journal article › Research › peer-review

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Synthesis of 11-carbon terpenoids in yeast using protein and metabolic engineering. / Ignea, Codruta; Pontini, Marianna; Motawia, Mohammed S.; Maffei, Massimo E; Makris, Antonios M; Kampranis, Sotirios C.

In: Nature Chemical Biology, Vol. 14, No. 12, 2018, p. 1090-1098.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Ignea, C, Pontini, M, Motawia, MS, Maffei, ME, Makris, AM & Kampranis, SC 2018, 'Synthesis of 11-carbon terpenoids in yeast using protein and metabolic engineering', Nature Chemical Biology, vol. 14, no. 12, pp. 1090-1098. https://doi.org/10.1038/s41589-018-0166-5

APA

Ignea, C., Pontini, M., Motawia, M. S., Maffei, M. E., Makris, A. M., & Kampranis, S. C. (2018). Synthesis of 11-carbon terpenoids in yeast using protein and metabolic engineering. Nature Chemical Biology, 14(12), 1090-1098. https://doi.org/10.1038/s41589-018-0166-5

Vancouver

Ignea C, Pontini M, Motawia MS, Maffei ME, Makris AM, Kampranis SC. Synthesis of 11-carbon terpenoids in yeast using protein and metabolic engineering. Nature Chemical Biology. 2018;14(12):1090-1098. https://doi.org/10.1038/s41589-018-0166-5

Author

Ignea, Codruta ; Pontini, Marianna ; Motawia, Mohammed S. ; Maffei, Massimo E ; Makris, Antonios M ; Kampranis, Sotirios C. / Synthesis of 11-carbon terpenoids in yeast using protein and metabolic engineering. In: Nature Chemical Biology. 2018 ; Vol. 14, No. 12. pp. 1090-1098.

Bibtex

@article{4fc78011644646db92e0995afc4a80d7,
title = "Synthesis of 11-carbon terpenoids in yeast using protein and metabolic engineering",
abstract = "One application of synthetic biology is the redesign of existing biological systems to acquire new functions. In this context, expanding the chemical code underlying key biosynthetic pathways will lead to the synthesis of compounds with new structures and potentially new biological activities. Terpenoids are a large group of specialized metabolites with numerous applications. Yet, being synthesized from five-carbon units, they are restricted to distinct classes that differ by five carbon atoms (C10, C15, C20, etc.). To expand the diversity of terpenoid structures, we engineered yeast cells to synthesize a noncanonical building block with 11 carbons, and produced 40 C11 terpene scaffolds that can form the basis for an entire terpenoid class. By identifying a single-residue switch that converts C10 plant monoterpene synthases to C11-specific enzymes, we engineered dedicated synthases for C11 terpene production. This approach will enable the systematic expansion of the chemical space accessed by terpenoids.",
author = "Codruta Ignea and Marianna Pontini and Motawia, {Mohammed S.} and Maffei, {Massimo E} and Makris, {Antonios M} and Kampranis, {Sotirios C.}",
year = "2018",
doi = "10.1038/s41589-018-0166-5",
language = "English",
volume = "14",
pages = "1090--1098",
journal = "Nature Chemical Biology",
issn = "1552-4450",
publisher = "nature publishing group",
number = "12",

}

RIS

TY - JOUR

T1 - Synthesis of 11-carbon terpenoids in yeast using protein and metabolic engineering

AU - Ignea, Codruta

AU - Pontini, Marianna

AU - Motawia, Mohammed S.

AU - Maffei, Massimo E

AU - Makris, Antonios M

AU - Kampranis, Sotirios C.

PY - 2018

Y1 - 2018

N2 - One application of synthetic biology is the redesign of existing biological systems to acquire new functions. In this context, expanding the chemical code underlying key biosynthetic pathways will lead to the synthesis of compounds with new structures and potentially new biological activities. Terpenoids are a large group of specialized metabolites with numerous applications. Yet, being synthesized from five-carbon units, they are restricted to distinct classes that differ by five carbon atoms (C10, C15, C20, etc.). To expand the diversity of terpenoid structures, we engineered yeast cells to synthesize a noncanonical building block with 11 carbons, and produced 40 C11 terpene scaffolds that can form the basis for an entire terpenoid class. By identifying a single-residue switch that converts C10 plant monoterpene synthases to C11-specific enzymes, we engineered dedicated synthases for C11 terpene production. This approach will enable the systematic expansion of the chemical space accessed by terpenoids.

AB - One application of synthetic biology is the redesign of existing biological systems to acquire new functions. In this context, expanding the chemical code underlying key biosynthetic pathways will lead to the synthesis of compounds with new structures and potentially new biological activities. Terpenoids are a large group of specialized metabolites with numerous applications. Yet, being synthesized from five-carbon units, they are restricted to distinct classes that differ by five carbon atoms (C10, C15, C20, etc.). To expand the diversity of terpenoid structures, we engineered yeast cells to synthesize a noncanonical building block with 11 carbons, and produced 40 C11 terpene scaffolds that can form the basis for an entire terpenoid class. By identifying a single-residue switch that converts C10 plant monoterpene synthases to C11-specific enzymes, we engineered dedicated synthases for C11 terpene production. This approach will enable the systematic expansion of the chemical space accessed by terpenoids.

U2 - 10.1038/s41589-018-0166-5

DO - 10.1038/s41589-018-0166-5

M3 - Journal article

C2 - 30429605

VL - 14

SP - 1090

EP - 1098

JO - Nature Chemical Biology

JF - Nature Chemical Biology

SN - 1552-4450

IS - 12

ER -

ID: 209366571