We are working on the dynamic assembly of biosynthetic enzyme complexes, termed metabolons, for the production of phytochemicals. Plants produce an immense number of bioactive compounds with potential for applications as pharmaceuticals, colorants and flavors. Despite of the chemical diversity of phytochemicals, their biosynthesis involves a highly modular assembly of specific enzyme classes such as oxidoreductases, cytochromes P450 and transferases. Assembly of metabolons facilitates direct transfer of substrates and intermediates between sequential enzymes, which results in increased flux devoid of concomitant release of toxic or labile intermediates. We are interested in understanding how plants assemble metabolons on-demand in response to environmental challenges.
Find more information about the group in the menu below.
- Structural organization We are studying the structural organization of metabolons and their different configurations. We are interested in general mechanisms regulating the assembly of metabolons such as scaffolding proteins, protein-protein interactions and the local membrane environment.
- Dynamic assembly We are uncovering the dynamics of metabolon assembly at the single molecule level. The diffusion of individual enzymes and entire metabolons provides kinetic insight on the assembly and disassembly. In the process, we are developing new platforms for studying protein-protein interactions, membrane sorting and binding of soluble enzymes.
- Functional consequences We apply our findings to study the functional consequences of enzyme engineering and lipid composition on substrate channeling using in vivo and in vitro systems. As part of this research topic we are optimizing biomimetic membrane systems for studying multienzyme complexes.
Current research projects
- Plant metabolon discovery, isolation and molecular characterization for optimizing metabolic highways, Novo Nordisk Foundation (PI: Tomas Laursen)
- Directing plant metabolism towards formation of high value bioactive products, Sapere Aude Starting Grant, Independent Research Fund Denmark (PI: Tomas Laursen)
- Optimization of specialized plant metabolite production, Novo Scholarship Programme (PI: Ketil Mathiasen Viborg)
Department of Plant and Environmental Sciences have various bachelor and master programs.
Partners and networks
- Biochemical engineering (Sotirios Kampranis)
- Cyanogenic glucosides, Vanilin and Carmine (Birger Lindberg Møller)
- Diterpenoids (Birger Lindberg Møller and Irini Pateraki)
- Triterpenoids (Søren Bak)
- Ecophysiology plasticity (Elisabeth Heather Neilson)
- Metabolic plasticity (Nanna Bjarnholt)
- Cryo Electron Microscopy (Bjørn Panyella Pedersen, Aarhus University, DK)
- Native Mass Spectrometry (Jonathan T. Hopper and Carol Robinson, Oxford University, UK)
- Neutron Reflectometry (Marité Cardenas, Malmö University, SE)
- SMALP technology (Timothy Dafforn, University of Birmingham, UK)
- Single Molecule Microscopy (Jay T. Groves, UC-Berkeley, USA and Nikos Hatzakis, University of Copenhagen, DK)
- Computational modeling (Flemming Jørgensen, University of Copenhagen, DK)