Reduction of antinutritional glucosinolates in Brassica oilseeds by mutation of genes encoding transporters

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Reduction of antinutritional glucosinolates in Brassica oilseeds by mutation of genes encoding transporters. / Nour-Eldin, Hussam Hassan; Madsen, Svend Roesen; Engelen, Steven; Jørgensen, Morten Egevang; Olsen, Carl Erik; Andersen, Jonathan Sonne; Seynnaeve, David; Verhoye, Thalia; Fulawka, Rudy; Denolf, Peter; Halkier, Barbara Ann.

In: Nature Biotechnology, Vol. 35, No. 4, 2017, p. 377-382.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Nour-Eldin, HH, Madsen, SR, Engelen, S, Jørgensen, ME, Olsen, CE, Andersen, JS, Seynnaeve, D, Verhoye, T, Fulawka, R, Denolf, P & Halkier, BA 2017, 'Reduction of antinutritional glucosinolates in Brassica oilseeds by mutation of genes encoding transporters', Nature Biotechnology, vol. 35, no. 4, pp. 377-382. https://doi.org/10.1038/nbt.3823

APA

Nour-Eldin, H. H., Madsen, S. R., Engelen, S., Jørgensen, M. E., Olsen, C. E., Andersen, J. S., Seynnaeve, D., Verhoye, T., Fulawka, R., Denolf, P., & Halkier, B. A. (2017). Reduction of antinutritional glucosinolates in Brassica oilseeds by mutation of genes encoding transporters. Nature Biotechnology, 35(4), 377-382. https://doi.org/10.1038/nbt.3823

Vancouver

Nour-Eldin HH, Madsen SR, Engelen S, Jørgensen ME, Olsen CE, Andersen JS et al. Reduction of antinutritional glucosinolates in Brassica oilseeds by mutation of genes encoding transporters. Nature Biotechnology. 2017;35(4):377-382. https://doi.org/10.1038/nbt.3823

Author

Nour-Eldin, Hussam Hassan ; Madsen, Svend Roesen ; Engelen, Steven ; Jørgensen, Morten Egevang ; Olsen, Carl Erik ; Andersen, Jonathan Sonne ; Seynnaeve, David ; Verhoye, Thalia ; Fulawka, Rudy ; Denolf, Peter ; Halkier, Barbara Ann. / Reduction of antinutritional glucosinolates in Brassica oilseeds by mutation of genes encoding transporters. In: Nature Biotechnology. 2017 ; Vol. 35, No. 4. pp. 377-382.

Bibtex

@article{b6e2b58d40d64aba9187cd91f806b01e,
title = "Reduction of antinutritional glucosinolates in Brassica oilseeds by mutation of genes encoding transporters",
abstract = "The nutritional value of Brassica seed meals is reduced by the presence of glucosinolates, which are toxic compounds involved in plant defense. Mutation of the genes encoding two glucosinolate transporters (GTRs) eliminated glucosinolates from Arabidopsis thaliana seeds, but translation of loss-of-function phenotypes into Brassica crops is challenging because Brassica is polyploid. We mutated one of seven and four of 12 GTR orthologs and reduced glucosinolate levels in seeds by 60-70% in two different Brassica species (Brassica rapa and Brassica juncea). Reduction in seed glucosinolates was stably inherited over multiple generations and maintained in field trials of two mutant populations at three locations. Successful translation of the gtr loss-of-function phenotype from model plant to two Brassica crops suggests that our transport engineering approach could be broadly applied to reduce seed glucosinolate content in other oilseed crops, such as Camelina sativa or Crambe abyssinica.",
keywords = "Brassica, Genetic Enhancement, Glucosinolates, Monosaccharide Transport Proteins, Mutation, Plant Oils, Plants, Genetically Modified, Seeds, Journal Article",
author = "Nour-Eldin, {Hussam Hassan} and Madsen, {Svend Roesen} and Steven Engelen and J{\o}rgensen, {Morten Egevang} and Olsen, {Carl Erik} and Andersen, {Jonathan Sonne} and David Seynnaeve and Thalia Verhoye and Rudy Fulawka and Peter Denolf and Halkier, {Barbara Ann}",
year = "2017",
doi = "10.1038/nbt.3823",
language = "English",
volume = "35",
pages = "377--382",
journal = "Nature Biotechnology",
issn = "1087-0156",
publisher = "nature publishing group",
number = "4",

}

RIS

TY - JOUR

T1 - Reduction of antinutritional glucosinolates in Brassica oilseeds by mutation of genes encoding transporters

AU - Nour-Eldin, Hussam Hassan

AU - Madsen, Svend Roesen

AU - Engelen, Steven

AU - Jørgensen, Morten Egevang

AU - Olsen, Carl Erik

AU - Andersen, Jonathan Sonne

AU - Seynnaeve, David

AU - Verhoye, Thalia

AU - Fulawka, Rudy

AU - Denolf, Peter

AU - Halkier, Barbara Ann

PY - 2017

Y1 - 2017

N2 - The nutritional value of Brassica seed meals is reduced by the presence of glucosinolates, which are toxic compounds involved in plant defense. Mutation of the genes encoding two glucosinolate transporters (GTRs) eliminated glucosinolates from Arabidopsis thaliana seeds, but translation of loss-of-function phenotypes into Brassica crops is challenging because Brassica is polyploid. We mutated one of seven and four of 12 GTR orthologs and reduced glucosinolate levels in seeds by 60-70% in two different Brassica species (Brassica rapa and Brassica juncea). Reduction in seed glucosinolates was stably inherited over multiple generations and maintained in field trials of two mutant populations at three locations. Successful translation of the gtr loss-of-function phenotype from model plant to two Brassica crops suggests that our transport engineering approach could be broadly applied to reduce seed glucosinolate content in other oilseed crops, such as Camelina sativa or Crambe abyssinica.

AB - The nutritional value of Brassica seed meals is reduced by the presence of glucosinolates, which are toxic compounds involved in plant defense. Mutation of the genes encoding two glucosinolate transporters (GTRs) eliminated glucosinolates from Arabidopsis thaliana seeds, but translation of loss-of-function phenotypes into Brassica crops is challenging because Brassica is polyploid. We mutated one of seven and four of 12 GTR orthologs and reduced glucosinolate levels in seeds by 60-70% in two different Brassica species (Brassica rapa and Brassica juncea). Reduction in seed glucosinolates was stably inherited over multiple generations and maintained in field trials of two mutant populations at three locations. Successful translation of the gtr loss-of-function phenotype from model plant to two Brassica crops suggests that our transport engineering approach could be broadly applied to reduce seed glucosinolate content in other oilseed crops, such as Camelina sativa or Crambe abyssinica.

KW - Brassica

KW - Genetic Enhancement

KW - Glucosinolates

KW - Monosaccharide Transport Proteins

KW - Mutation

KW - Plant Oils

KW - Plants, Genetically Modified

KW - Seeds

KW - Journal Article

U2 - 10.1038/nbt.3823

DO - 10.1038/nbt.3823

M3 - Journal article

C2 - 28288105

VL - 35

SP - 377

EP - 382

JO - Nature Biotechnology

JF - Nature Biotechnology

SN - 1087-0156

IS - 4

ER -

ID: 180759552