Phylogenetic relationships in the Sorghum genus based on sequencing of the chloroplast and nuclear genes
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Phylogenetic relationships in the Sorghum genus based on sequencing of the chloroplast and nuclear genes. / Ananda, Galaihalage; Norton, Sally; Blomstedt, Cecilia; Furtado, Agnelo; Møller, Birger; Gleadow, Roslyn; Henry, Robert.
In: Plant Genome, Vol. 14, No. 3, e20123, 2021.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Phylogenetic relationships in the Sorghum genus based on sequencing of the chloroplast and nuclear genes
AU - Ananda, Galaihalage
AU - Norton, Sally
AU - Blomstedt, Cecilia
AU - Furtado, Agnelo
AU - Møller, Birger
AU - Gleadow, Roslyn
AU - Henry, Robert
PY - 2021
Y1 - 2021
N2 - Sorghum [Sorghum bicolor (L.) Moench] is an important food crop with a diverse gene pool residing in its wild relatives. A total of 15 sorghum accessions from the unexploited wild gene pool of the Sorghum genus, representing the five subgenera, were sequenced, and the complete chloroplast genomes and 99 common single-copy concatenated nuclear genes were assembled. Annotation of the chloroplast genomes identified a total of 81 protein-coding genes, 38 tRNA, and four rRNA genes. The gene content and gene order among the species was identical. A total of 153 nonsynonymous amino acid changes in 40 genes were identified across the species. Phylogenetic analysis of both the whole chloroplast genome and nuclear genes revealed a similar topology with two distinct clades within the genus. The species within the subgenera Eusorghum, Chaetosorghum, and Heterosorghum clustered in one clade, whereas the species within the subgenera Parasorghum and Stiposorghum clustered in a second clade. However, the subgenera Parasorghum and Stiposorghum were not monophyletic, suggesting the need for further research to resolve the relationships within this group. The close relationship between the two monotypic subgenera Chaetosorghum and Heterosorghum suggests that species within these subgenera could be considered as one group. This analysis provides an improved understanding of the genetic relationships within the Sorghum genus and defines diversity in wild sorghum species that may be useful for crop improvement.
AB - Sorghum [Sorghum bicolor (L.) Moench] is an important food crop with a diverse gene pool residing in its wild relatives. A total of 15 sorghum accessions from the unexploited wild gene pool of the Sorghum genus, representing the five subgenera, were sequenced, and the complete chloroplast genomes and 99 common single-copy concatenated nuclear genes were assembled. Annotation of the chloroplast genomes identified a total of 81 protein-coding genes, 38 tRNA, and four rRNA genes. The gene content and gene order among the species was identical. A total of 153 nonsynonymous amino acid changes in 40 genes were identified across the species. Phylogenetic analysis of both the whole chloroplast genome and nuclear genes revealed a similar topology with two distinct clades within the genus. The species within the subgenera Eusorghum, Chaetosorghum, and Heterosorghum clustered in one clade, whereas the species within the subgenera Parasorghum and Stiposorghum clustered in a second clade. However, the subgenera Parasorghum and Stiposorghum were not monophyletic, suggesting the need for further research to resolve the relationships within this group. The close relationship between the two monotypic subgenera Chaetosorghum and Heterosorghum suggests that species within these subgenera could be considered as one group. This analysis provides an improved understanding of the genetic relationships within the Sorghum genus and defines diversity in wild sorghum species that may be useful for crop improvement.
KW - GENOME SEQUENCES
KW - POACEAE
KW - BICOLOR
KW - EVOLUTION
KW - MACROSPERMUM
KW - ANNOTATION
KW - GRASSES
KW - RPOC2
KW - RPL22
KW - ITS1
U2 - 10.1002/tpg2.20123
DO - 10.1002/tpg2.20123
M3 - Journal article
C2 - 34323394
VL - 14
JO - Crop Science
JF - Crop Science
SN - 0011-183X
IS - 3
M1 - e20123
ER -
ID: 275882447