Multiplexed microRNA detection using lanthanide-labeled DNA probes and laser ablation inductively coupled plasma mass spectrometry

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Multiplexed microRNA detection using lanthanide-labeled DNA probes and laser ablation inductively coupled plasma mass spectrometry. / de Bang, Thomas Christian; Shah, Pratik; Cho, Seok Keun; Yang, Seong Wook; Husted, Søren.

In: Analytical Chemistry, Vol. 86, No. 14, 2014, p. 6823-6826.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

de Bang, TC, Shah, P, Cho, SK, Yang, SW & Husted, S 2014, 'Multiplexed microRNA detection using lanthanide-labeled DNA probes and laser ablation inductively coupled plasma mass spectrometry', Analytical Chemistry, vol. 86, no. 14, pp. 6823-6826. https://doi.org/10.1021/ac5017166

APA

de Bang, T. C., Shah, P., Cho, S. K., Yang, S. W., & Husted, S. (2014). Multiplexed microRNA detection using lanthanide-labeled DNA probes and laser ablation inductively coupled plasma mass spectrometry. Analytical Chemistry, 86(14), 6823-6826. https://doi.org/10.1021/ac5017166

Vancouver

de Bang TC, Shah P, Cho SK, Yang SW, Husted S. Multiplexed microRNA detection using lanthanide-labeled DNA probes and laser ablation inductively coupled plasma mass spectrometry. Analytical Chemistry. 2014;86(14):6823-6826. https://doi.org/10.1021/ac5017166

Author

de Bang, Thomas Christian ; Shah, Pratik ; Cho, Seok Keun ; Yang, Seong Wook ; Husted, Søren. / Multiplexed microRNA detection using lanthanide-labeled DNA probes and laser ablation inductively coupled plasma mass spectrometry. In: Analytical Chemistry. 2014 ; Vol. 86, No. 14. pp. 6823-6826.

Bibtex

@article{a6d18ce3308447ef81e2bd7749306aac,
title = "Multiplexed microRNA detection using lanthanide-labeled DNA probes and laser ablation inductively coupled plasma mass spectrometry",
abstract = "In the past decade, microRNAs (miRNAs) have drawn increasing attention due to their role in regulation of gene expression. Especially, their potential as biomarkers in disease diagnostics has motivated miRNA research, including the development of simple, accurate, and sensitive detection methods. The narrow size range of miRNAs (20-24 nucleotides) combined with the chemical properties of conventional reporter tags has hampered the development of multiplexed miRNA assays. In this study, we have used lanthanide-labeled DNA probes for the detection of miRNAs on membranes using laser ablation inductively coupled plasma mass spectrometry (LA-ICPMS). Three miRNAs from Arabidopsis thaliana were analyzed simultaneously with high specificity, and the sensitivity of the method was comparable to radioactive detection (low femtomol range). The perspective of the developed method is highly multiplexed and quantitative miRNA analysis with high specificity and sensitivity.",
author = "{de Bang}, {Thomas Christian} and Pratik Shah and Cho, {Seok Keun} and Yang, {Seong Wook} and S{\o}ren Husted",
year = "2014",
doi = "10.1021/ac5017166",
language = "English",
volume = "86",
pages = "6823--6826",
journal = "Industrial And Engineering Chemistry Analytical Edition",
issn = "0003-2700",
publisher = "American Chemical Society",
number = "14",

}

RIS

TY - JOUR

T1 - Multiplexed microRNA detection using lanthanide-labeled DNA probes and laser ablation inductively coupled plasma mass spectrometry

AU - de Bang, Thomas Christian

AU - Shah, Pratik

AU - Cho, Seok Keun

AU - Yang, Seong Wook

AU - Husted, Søren

PY - 2014

Y1 - 2014

N2 - In the past decade, microRNAs (miRNAs) have drawn increasing attention due to their role in regulation of gene expression. Especially, their potential as biomarkers in disease diagnostics has motivated miRNA research, including the development of simple, accurate, and sensitive detection methods. The narrow size range of miRNAs (20-24 nucleotides) combined with the chemical properties of conventional reporter tags has hampered the development of multiplexed miRNA assays. In this study, we have used lanthanide-labeled DNA probes for the detection of miRNAs on membranes using laser ablation inductively coupled plasma mass spectrometry (LA-ICPMS). Three miRNAs from Arabidopsis thaliana were analyzed simultaneously with high specificity, and the sensitivity of the method was comparable to radioactive detection (low femtomol range). The perspective of the developed method is highly multiplexed and quantitative miRNA analysis with high specificity and sensitivity.

AB - In the past decade, microRNAs (miRNAs) have drawn increasing attention due to their role in regulation of gene expression. Especially, their potential as biomarkers in disease diagnostics has motivated miRNA research, including the development of simple, accurate, and sensitive detection methods. The narrow size range of miRNAs (20-24 nucleotides) combined with the chemical properties of conventional reporter tags has hampered the development of multiplexed miRNA assays. In this study, we have used lanthanide-labeled DNA probes for the detection of miRNAs on membranes using laser ablation inductively coupled plasma mass spectrometry (LA-ICPMS). Three miRNAs from Arabidopsis thaliana were analyzed simultaneously with high specificity, and the sensitivity of the method was comparable to radioactive detection (low femtomol range). The perspective of the developed method is highly multiplexed and quantitative miRNA analysis with high specificity and sensitivity.

U2 - 10.1021/ac5017166

DO - 10.1021/ac5017166

M3 - Journal article

C2 - 24945747

AN - SCOPUS:84904339793

VL - 86

SP - 6823

EP - 6826

JO - Industrial And Engineering Chemistry Analytical Edition

JF - Industrial And Engineering Chemistry Analytical Edition

SN - 0003-2700

IS - 14

ER -

ID: 130102304