Identification of nitric oxide mediated defense signaling and its microRNA mediated regulation during Phytophthora capsici infection in black pepper

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Identification of nitric oxide mediated defense signaling and its microRNA mediated regulation during Phytophthora capsici infection in black pepper. / Asha, Srinivasan; Kattupalli, Divya; Vijayanathan, Mallika; Soniya, E. V.

In: Physiology and Molecular Biology of Plants, Vol. 30, 2024, p. 33-47.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Asha, S, Kattupalli, D, Vijayanathan, M & Soniya, EV 2024, 'Identification of nitric oxide mediated defense signaling and its microRNA mediated regulation during Phytophthora capsici infection in black pepper', Physiology and Molecular Biology of Plants, vol. 30, pp. 33-47. https://doi.org/10.1007/s12298-024-01414-z

APA

Asha, S., Kattupalli, D., Vijayanathan, M., & Soniya, E. V. (2024). Identification of nitric oxide mediated defense signaling and its microRNA mediated regulation during Phytophthora capsici infection in black pepper. Physiology and Molecular Biology of Plants, 30, 33-47. https://doi.org/10.1007/s12298-024-01414-z

Vancouver

Asha S, Kattupalli D, Vijayanathan M, Soniya EV. Identification of nitric oxide mediated defense signaling and its microRNA mediated regulation during Phytophthora capsici infection in black pepper. Physiology and Molecular Biology of Plants. 2024;30:33-47. https://doi.org/10.1007/s12298-024-01414-z

Author

Asha, Srinivasan ; Kattupalli, Divya ; Vijayanathan, Mallika ; Soniya, E. V. / Identification of nitric oxide mediated defense signaling and its microRNA mediated regulation during Phytophthora capsici infection in black pepper. In: Physiology and Molecular Biology of Plants. 2024 ; Vol. 30. pp. 33-47.

Bibtex

@article{508c4e8e9ab545029dfaaae7eb9b1e4c,
title = "Identification of nitric oxide mediated defense signaling and its microRNA mediated regulation during Phytophthora capsici infection in black pepper",
abstract = "Nitric oxide plays a significant role in the defense signaling during pathogen interaction in plants. Quick wilt disease is a devastating disease of black pepper, and leads to sudden mortality of pepper vines in plantations. In this study, the role of nitric oxide was studied during Phytophthora capsici infection in black pepper variety Panniyur-1. Nitric oxide was detected from the different histological sections of P. capsici infected leaves. Furthermore, the genome-wide transcriptome analysis characterized typical domain architect and structural features of nitrate reductase (NR) and nitric oxide associated 1 (NOA1) gene that are involved in nitric oxide biosynthesis in black pepper. Despite the upregulation of nitrate reductase (Pn1_NR), a reduced expression of Pn1_NOA1 was detected in the P. capsici infected black pepper leaf. Subsequent sRNAome-assisted in silico analysis revealed possible microRNA mediated regulation of Pn1_NOA mRNAs. Furthermore, sRNA/miRNA mediated cleavage on Pn1_NOA1 mRNA was validated through modified 5' RLM RACE experiments. Several hormone-responsive cis-regulatory elements involved in stress response was detected from the promoter regions of Pn_NOA1, Pn_NR1 and Pn_NR2 genes. Our results revealed the role of nitric oxide during stress response of P. capsici infection in black pepper, and key genes involved in nitric oxide biosynthesis and their post-transcriptional regulatory mechanisms.",
keywords = "Black pepper, Nitrate reductase, Nitric oxide, NOA, Phytophthora capsici, Quick wilt, Transcriptome",
author = "Srinivasan Asha and Divya Kattupalli and Mallika Vijayanathan and Soniya, {E. V.}",
note = "Publisher Copyright: {\textcopyright} Prof. H.S. Srivastava Foundation for Science and Society 2024.",
year = "2024",
doi = "10.1007/s12298-024-01414-z",
language = "English",
volume = "30",
pages = "33--47",
journal = "Physiology and Molecular Biology of Plants",
issn = "0971-5894",
publisher = "Springer",

}

RIS

TY - JOUR

T1 - Identification of nitric oxide mediated defense signaling and its microRNA mediated regulation during Phytophthora capsici infection in black pepper

AU - Asha, Srinivasan

AU - Kattupalli, Divya

AU - Vijayanathan, Mallika

AU - Soniya, E. V.

N1 - Publisher Copyright: © Prof. H.S. Srivastava Foundation for Science and Society 2024.

PY - 2024

Y1 - 2024

N2 - Nitric oxide plays a significant role in the defense signaling during pathogen interaction in plants. Quick wilt disease is a devastating disease of black pepper, and leads to sudden mortality of pepper vines in plantations. In this study, the role of nitric oxide was studied during Phytophthora capsici infection in black pepper variety Panniyur-1. Nitric oxide was detected from the different histological sections of P. capsici infected leaves. Furthermore, the genome-wide transcriptome analysis characterized typical domain architect and structural features of nitrate reductase (NR) and nitric oxide associated 1 (NOA1) gene that are involved in nitric oxide biosynthesis in black pepper. Despite the upregulation of nitrate reductase (Pn1_NR), a reduced expression of Pn1_NOA1 was detected in the P. capsici infected black pepper leaf. Subsequent sRNAome-assisted in silico analysis revealed possible microRNA mediated regulation of Pn1_NOA mRNAs. Furthermore, sRNA/miRNA mediated cleavage on Pn1_NOA1 mRNA was validated through modified 5' RLM RACE experiments. Several hormone-responsive cis-regulatory elements involved in stress response was detected from the promoter regions of Pn_NOA1, Pn_NR1 and Pn_NR2 genes. Our results revealed the role of nitric oxide during stress response of P. capsici infection in black pepper, and key genes involved in nitric oxide biosynthesis and their post-transcriptional regulatory mechanisms.

AB - Nitric oxide plays a significant role in the defense signaling during pathogen interaction in plants. Quick wilt disease is a devastating disease of black pepper, and leads to sudden mortality of pepper vines in plantations. In this study, the role of nitric oxide was studied during Phytophthora capsici infection in black pepper variety Panniyur-1. Nitric oxide was detected from the different histological sections of P. capsici infected leaves. Furthermore, the genome-wide transcriptome analysis characterized typical domain architect and structural features of nitrate reductase (NR) and nitric oxide associated 1 (NOA1) gene that are involved in nitric oxide biosynthesis in black pepper. Despite the upregulation of nitrate reductase (Pn1_NR), a reduced expression of Pn1_NOA1 was detected in the P. capsici infected black pepper leaf. Subsequent sRNAome-assisted in silico analysis revealed possible microRNA mediated regulation of Pn1_NOA mRNAs. Furthermore, sRNA/miRNA mediated cleavage on Pn1_NOA1 mRNA was validated through modified 5' RLM RACE experiments. Several hormone-responsive cis-regulatory elements involved in stress response was detected from the promoter regions of Pn_NOA1, Pn_NR1 and Pn_NR2 genes. Our results revealed the role of nitric oxide during stress response of P. capsici infection in black pepper, and key genes involved in nitric oxide biosynthesis and their post-transcriptional regulatory mechanisms.

KW - Black pepper

KW - Nitrate reductase

KW - Nitric oxide

KW - NOA

KW - Phytophthora capsici

KW - Quick wilt

KW - Transcriptome

U2 - 10.1007/s12298-024-01414-z

DO - 10.1007/s12298-024-01414-z

M3 - Journal article

AN - SCOPUS:85184869005

VL - 30

SP - 33

EP - 47

JO - Physiology and Molecular Biology of Plants

JF - Physiology and Molecular Biology of Plants

SN - 0971-5894

ER -

ID: 383397096