Identification of circular RNAs from the parental genes involved in multiple aspects of cellular metabolism in barley
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Identification of circular RNAs from the parental genes involved in multiple aspects of cellular metabolism in barley. / Darbani Shirvanehdeh, Behrooz; Noeparvar, Shahin; Borg, Søren.
In: Frontiers in Plant Science, Vol. 7, 776, 2016.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Identification of circular RNAs from the parental genes involved in multiple aspects of cellular metabolism in barley
AU - Darbani Shirvanehdeh, Behrooz
AU - Noeparvar, Shahin
AU - Borg, Søren
PY - 2016
Y1 - 2016
N2 - RNA circularization made by head-to-tail back-splicing events is involved in the regulation of gene expression from transcriptional to post-translational levels. By exploiting RNA-Seq data and down-stream analysis, we shed light on the importance of circular RNAs in plants. The results introduce circular RNAs as novel interactors in the regulation of gene expression in plants and imply the comprehensiveness of this regulatory pathway by identifying circular RNAs for a diverse set of genes. These genes are involved in several aspects of cellular metabolism as hormonal signaling, intracellular protein sorting, carbohydrate metabolism and cell-wall biogenesis, respiration, amino acid biosynthesis, transcription and translation, and protein ubiquitination. Additionally, these parental loci of circular RNAs, from both nuclear and mitochondrial genomes, encode for different transcript classes including protein coding transcripts, microRNA, rRNA, and long non-coding/microprotein coding RNAs. The results shed light on the mitochondrial exonic circular RNAs and imply the importance of circular RNAs for regulation of mitochondrial genes. Importantly, we introduce circular RNAs in barley and elucidate their cellular-level alterations across tissues and in response to micronutrients iron and zinc. In further support of circular RNAs’ functional roles in plants, we report several cases where fluctuations of circRNAs do not correlate with the levels of their parental-loci encoded linear transcripts.
AB - RNA circularization made by head-to-tail back-splicing events is involved in the regulation of gene expression from transcriptional to post-translational levels. By exploiting RNA-Seq data and down-stream analysis, we shed light on the importance of circular RNAs in plants. The results introduce circular RNAs as novel interactors in the regulation of gene expression in plants and imply the comprehensiveness of this regulatory pathway by identifying circular RNAs for a diverse set of genes. These genes are involved in several aspects of cellular metabolism as hormonal signaling, intracellular protein sorting, carbohydrate metabolism and cell-wall biogenesis, respiration, amino acid biosynthesis, transcription and translation, and protein ubiquitination. Additionally, these parental loci of circular RNAs, from both nuclear and mitochondrial genomes, encode for different transcript classes including protein coding transcripts, microRNA, rRNA, and long non-coding/microprotein coding RNAs. The results shed light on the mitochondrial exonic circular RNAs and imply the importance of circular RNAs for regulation of mitochondrial genes. Importantly, we introduce circular RNAs in barley and elucidate their cellular-level alterations across tissues and in response to micronutrients iron and zinc. In further support of circular RNAs’ functional roles in plants, we report several cases where fluctuations of circRNAs do not correlate with the levels of their parental-loci encoded linear transcripts.
KW - Circular RNAs
KW - Coding and non-coding transcripts
KW - Leaves
KW - Micronutrients
KW - Mitochondria
KW - Seeds
KW - Transfer cells
U2 - 10.3389/fpls.2016.00776
DO - 10.3389/fpls.2016.00776
M3 - Journal article
C2 - 27375638
AN - SCOPUS:84971619832
VL - 7
JO - Frontiers in Plant Science
JF - Frontiers in Plant Science
SN - 1664-462X
M1 - 776
ER -
ID: 178846722