High level of expression of recombinant human granulocyte-macrophage colony stimulating factor in transgenic rice cell suspension culture
Research output: Contribution to journal › Journal article › Research › peer-review
Recombinant human granulocyte-macrophage colony stimulating factor (hGM-CSF) has been previously produced in tobacco cell suspension cultures. However, the amount of hGM-CSF accumulated in the culture medium dropped quickly from its maximum of 150 microg/L at 5 d after incubation. To overcome this problem, we sought an expression system in which heterologous gene expression could be induced at high levels. We selected a rice amylase expression system in which the promoter Ramy3D is induced to express recombinant protein by sucrose starvation. This induction system was found to give good yield of recombinant hGM-CSF in transgenic rice cell suspension culture and protease activity of this culture medium was low compared to that of tobacco culture system.
Original language | English |
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Journal | Biotechnology and Bioengineering (Print) |
Volume | 82 |
Issue number | 7 |
Pages (from-to) | 778-83 |
Number of pages | 6 |
ISSN | 0006-3592 |
DOIs | |
Publication status | Published - 30 Jun 2003 |
- Cells, Cultured, Endopeptidases, Gene Expression Regulation, Plant, Granulocyte-Macrophage Colony-Stimulating Factor, Humans, Oryza, Plants, Genetically Modified, Protein Engineering, Quality Control, Recombinant Proteins, Tobacco
Research areas
ID: 162127945