CYP303A1 has a conserved function in adult eclosion in Locusta migratoria and Drosophila melanogaster

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Standard

CYP303A1 has a conserved function in adult eclosion in Locusta migratoria and Drosophila melanogaster. / Wu, Lixian; Jia, Qiangqiang; Zhang, Xubo; Zhang, Xueyao; Liu, Suning; Park, Yoonseong; Feyereisen, René; Zhu, Kun Yan; Ma, Enbo; Zhang, Jianzhen; Li, Sheng.

In: Insect Biochemistry and Molecular Biology, Vol. 113, 103210, 10.2019, p. 1-9.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Wu, L, Jia, Q, Zhang, X, Zhang, X, Liu, S, Park, Y, Feyereisen, R, Zhu, KY, Ma, E, Zhang, J & Li, S 2019, 'CYP303A1 has a conserved function in adult eclosion in Locusta migratoria and Drosophila melanogaster', Insect Biochemistry and Molecular Biology, vol. 113, 103210, pp. 1-9. https://doi.org/10.1016/j.ibmb.2019.103210

APA

Wu, L., Jia, Q., Zhang, X., Zhang, X., Liu, S., Park, Y., Feyereisen, R., Zhu, K. Y., Ma, E., Zhang, J., & Li, S. (2019). CYP303A1 has a conserved function in adult eclosion in Locusta migratoria and Drosophila melanogaster. Insect Biochemistry and Molecular Biology, 113, 1-9. [103210]. https://doi.org/10.1016/j.ibmb.2019.103210

Vancouver

Wu L, Jia Q, Zhang X, Zhang X, Liu S, Park Y et al. CYP303A1 has a conserved function in adult eclosion in Locusta migratoria and Drosophila melanogaster. Insect Biochemistry and Molecular Biology. 2019 Oct;113:1-9. 103210. https://doi.org/10.1016/j.ibmb.2019.103210

Author

Wu, Lixian ; Jia, Qiangqiang ; Zhang, Xubo ; Zhang, Xueyao ; Liu, Suning ; Park, Yoonseong ; Feyereisen, René ; Zhu, Kun Yan ; Ma, Enbo ; Zhang, Jianzhen ; Li, Sheng. / CYP303A1 has a conserved function in adult eclosion in Locusta migratoria and Drosophila melanogaster. In: Insect Biochemistry and Molecular Biology. 2019 ; Vol. 113. pp. 1-9.

Bibtex

@article{e156aa528a6e4a3a9128bbaf79b898f4,
title = "CYP303A1 has a conserved function in adult eclosion in Locusta migratoria and Drosophila melanogaster",
abstract = "Insect cytochrome P450 monooxygenases (CYPs) play essential roles in both xenobiotic metabolism and developmental processes. However, the exact physiological function of many CYP genes remains largely unknown. Screening the expression of the CYP genes from the CYP2 and mitochondrial CYP clans of Drosophila melanogaster revealed that Cyp303a1 is highly expressed in the pupal stage. Knockdown of CYP303A1 transcripts by RNAi using the Gal4/UAS system with a ubiquitous driver (tubulin-Gal4) in Drosophila or by dsRNA injection in the last nymph stage of Locusta migratoria resulted in severe defects in eclosion and lethality during and after adult emergence. In Drosophila, tissue-specific RNAi of Cyp303a1 with a wing-specific driver (MS1096-Gal4) revealed that Cyp303a1 was essential for wing extension. Stage-specific RNAi of Cyp303a1 using Gal80ts for thermal-dependent-suppression found that the expression of Cyp303a1 at the middle pupal stage was absolutely required. Meanwhile, Cyp303a1 mutants exhibited more than 80% lethality at the late embryonic development stages. Embryonic lethality of the Cyp303a1 mutants was fully rescued by the ubiquitous overexpression of exogenous Cyp303a1. Taken together, we conclude that Cyp303a1 is indispensable for embryonic development and adult eclosion in D. melanogaster, the latter role being conserved over 400 million years of insect evolution.",
keywords = "Adult eclosion, CYP303A1, Drosophila melanogaster, Embryonic development, Locusta migratoria",
author = "Lixian Wu and Qiangqiang Jia and Xubo Zhang and Xueyao Zhang and Suning Liu and Yoonseong Park and Ren{\'e} Feyereisen and Zhu, {Kun Yan} and Enbo Ma and Jianzhen Zhang and Sheng Li",
year = "2019",
month = oct,
doi = "10.1016/j.ibmb.2019.103210",
language = "English",
volume = "113",
pages = "1--9",
journal = "Insect Biochemistry and Molecular Biology",
issn = "0965-1748",
publisher = "Pergamon Press",

}

RIS

TY - JOUR

T1 - CYP303A1 has a conserved function in adult eclosion in Locusta migratoria and Drosophila melanogaster

AU - Wu, Lixian

AU - Jia, Qiangqiang

AU - Zhang, Xubo

AU - Zhang, Xueyao

AU - Liu, Suning

AU - Park, Yoonseong

AU - Feyereisen, René

AU - Zhu, Kun Yan

AU - Ma, Enbo

AU - Zhang, Jianzhen

AU - Li, Sheng

PY - 2019/10

Y1 - 2019/10

N2 - Insect cytochrome P450 monooxygenases (CYPs) play essential roles in both xenobiotic metabolism and developmental processes. However, the exact physiological function of many CYP genes remains largely unknown. Screening the expression of the CYP genes from the CYP2 and mitochondrial CYP clans of Drosophila melanogaster revealed that Cyp303a1 is highly expressed in the pupal stage. Knockdown of CYP303A1 transcripts by RNAi using the Gal4/UAS system with a ubiquitous driver (tubulin-Gal4) in Drosophila or by dsRNA injection in the last nymph stage of Locusta migratoria resulted in severe defects in eclosion and lethality during and after adult emergence. In Drosophila, tissue-specific RNAi of Cyp303a1 with a wing-specific driver (MS1096-Gal4) revealed that Cyp303a1 was essential for wing extension. Stage-specific RNAi of Cyp303a1 using Gal80ts for thermal-dependent-suppression found that the expression of Cyp303a1 at the middle pupal stage was absolutely required. Meanwhile, Cyp303a1 mutants exhibited more than 80% lethality at the late embryonic development stages. Embryonic lethality of the Cyp303a1 mutants was fully rescued by the ubiquitous overexpression of exogenous Cyp303a1. Taken together, we conclude that Cyp303a1 is indispensable for embryonic development and adult eclosion in D. melanogaster, the latter role being conserved over 400 million years of insect evolution.

AB - Insect cytochrome P450 monooxygenases (CYPs) play essential roles in both xenobiotic metabolism and developmental processes. However, the exact physiological function of many CYP genes remains largely unknown. Screening the expression of the CYP genes from the CYP2 and mitochondrial CYP clans of Drosophila melanogaster revealed that Cyp303a1 is highly expressed in the pupal stage. Knockdown of CYP303A1 transcripts by RNAi using the Gal4/UAS system with a ubiquitous driver (tubulin-Gal4) in Drosophila or by dsRNA injection in the last nymph stage of Locusta migratoria resulted in severe defects in eclosion and lethality during and after adult emergence. In Drosophila, tissue-specific RNAi of Cyp303a1 with a wing-specific driver (MS1096-Gal4) revealed that Cyp303a1 was essential for wing extension. Stage-specific RNAi of Cyp303a1 using Gal80ts for thermal-dependent-suppression found that the expression of Cyp303a1 at the middle pupal stage was absolutely required. Meanwhile, Cyp303a1 mutants exhibited more than 80% lethality at the late embryonic development stages. Embryonic lethality of the Cyp303a1 mutants was fully rescued by the ubiquitous overexpression of exogenous Cyp303a1. Taken together, we conclude that Cyp303a1 is indispensable for embryonic development and adult eclosion in D. melanogaster, the latter role being conserved over 400 million years of insect evolution.

KW - Adult eclosion

KW - CYP303A1

KW - Drosophila melanogaster

KW - Embryonic development

KW - Locusta migratoria

U2 - 10.1016/j.ibmb.2019.103210

DO - 10.1016/j.ibmb.2019.103210

M3 - Journal article

C2 - 31422152

AN - SCOPUS:85071319713

VL - 113

SP - 1

EP - 9

JO - Insect Biochemistry and Molecular Biology

JF - Insect Biochemistry and Molecular Biology

SN - 0965-1748

M1 - 103210

ER -

ID: 234149746