Cyanogenesis in the Sorghum Genus: From Genotype to Phenotype
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Cyanogenesis in the Sorghum Genus : From Genotype to Phenotype. / Cowan, Max; Møller, Birger Lindberg; Norton, Sally; Knudsen, Camilla; Crocoll, Christoph; Furtado, Agnelo; Henry, Robert; Blomstedt, Cecilia; Gleadow, Roslyn M.
In: Genes, Vol. 13, 140, 2022.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Cyanogenesis in the Sorghum Genus
T2 - From Genotype to Phenotype
AU - Cowan, Max
AU - Møller, Birger Lindberg
AU - Norton, Sally
AU - Knudsen, Camilla
AU - Crocoll, Christoph
AU - Furtado, Agnelo
AU - Henry, Robert
AU - Blomstedt, Cecilia
AU - Gleadow, Roslyn M.
N1 - Publisher Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2022
Y1 - 2022
N2 - Domestication has resulted in a loss of genetic diversity in our major food crops, leading to susceptibility to biotic and abiotic stresses linked with climate change. Crop wild relatives (CWR) may provide a source of novel genes potentially important for re-gaining climate resilience. Sorghum bicolor is an important cereal crop with wild relatives that are endemic to Australia. Sorghum bicolor is cyanogenic, but the cyanogenic status of wild Sorghum species is not well known. In this study, leaves of wild species endemic in Australia are screened for the presence of the cyanogenic glucoside dhurrin. The direct measurement of dhurrin content and the potential for dhurrin-derived HCN release (HCNp) showed that all the tested Australian wild species were essentially phenotypically acyanogenic. The unexpected low dhurrin content may reflect the variable and generally nutrient-poor environments in which they are growing in nature. Genome sequencing of six CWR and PCR amplification of the CYP79A1 gene from additional species showed that a high conservation of key amino acids is required for correct protein function and dhurrin synthesis, pointing to the transcriptional regulation of the cyanogenic phenotype in wild sorghum as previously shown in elite sorghum.
AB - Domestication has resulted in a loss of genetic diversity in our major food crops, leading to susceptibility to biotic and abiotic stresses linked with climate change. Crop wild relatives (CWR) may provide a source of novel genes potentially important for re-gaining climate resilience. Sorghum bicolor is an important cereal crop with wild relatives that are endemic to Australia. Sorghum bicolor is cyanogenic, but the cyanogenic status of wild Sorghum species is not well known. In this study, leaves of wild species endemic in Australia are screened for the presence of the cyanogenic glucoside dhurrin. The direct measurement of dhurrin content and the potential for dhurrin-derived HCN release (HCNp) showed that all the tested Australian wild species were essentially phenotypically acyanogenic. The unexpected low dhurrin content may reflect the variable and generally nutrient-poor environments in which they are growing in nature. Genome sequencing of six CWR and PCR amplification of the CYP79A1 gene from additional species showed that a high conservation of key amino acids is required for correct protein function and dhurrin synthesis, pointing to the transcriptional regulation of the cyanogenic phenotype in wild sorghum as previously shown in elite sorghum.
KW - Cyanogenesis
KW - Dhurrin
KW - Sorghum bicolor
KW - Wild crop relatives
U2 - 10.3390/genes13010140
DO - 10.3390/genes13010140
M3 - Journal article
C2 - 35052482
AN - SCOPUS:85123022709
VL - 13
JO - Genes
JF - Genes
SN - 2073-4425
M1 - 140
ER -
ID: 291357452