Biosynthesis of chlorophyll c in a dinoflagellate and heterologous production in planta

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Biosynthesis of chlorophyll c in a dinoflagellate and heterologous production in planta. / Jinkerson, Robert E.; Poveda-Huertes, Daniel; Cooney, Elizabeth C.; Cho, Anna; Ochoa-Fernandez, Rocio; Keeling, Patrick J.; Xiang, Tingting; Andersen-Ranberg, Johan.

In: Current Biology, Vol. 34, No. 3, 2024, p. 594-605.e4.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Jinkerson, RE, Poveda-Huertes, D, Cooney, EC, Cho, A, Ochoa-Fernandez, R, Keeling, PJ, Xiang, T & Andersen-Ranberg, J 2024, 'Biosynthesis of chlorophyll c in a dinoflagellate and heterologous production in planta', Current Biology, vol. 34, no. 3, pp. 594-605.e4. https://doi.org/10.1016/j.cub.2023.12.068

APA

Jinkerson, R. E., Poveda-Huertes, D., Cooney, E. C., Cho, A., Ochoa-Fernandez, R., Keeling, P. J., Xiang, T., & Andersen-Ranberg, J. (2024). Biosynthesis of chlorophyll c in a dinoflagellate and heterologous production in planta. Current Biology, 34(3), 594-605.e4. https://doi.org/10.1016/j.cub.2023.12.068

Vancouver

Jinkerson RE, Poveda-Huertes D, Cooney EC, Cho A, Ochoa-Fernandez R, Keeling PJ et al. Biosynthesis of chlorophyll c in a dinoflagellate and heterologous production in planta. Current Biology. 2024;34(3):594-605.e4. https://doi.org/10.1016/j.cub.2023.12.068

Author

Jinkerson, Robert E. ; Poveda-Huertes, Daniel ; Cooney, Elizabeth C. ; Cho, Anna ; Ochoa-Fernandez, Rocio ; Keeling, Patrick J. ; Xiang, Tingting ; Andersen-Ranberg, Johan. / Biosynthesis of chlorophyll c in a dinoflagellate and heterologous production in planta. In: Current Biology. 2024 ; Vol. 34, No. 3. pp. 594-605.e4.

Bibtex

@article{5d231f6012fb4bc59d6d383300733ae6,
title = "Biosynthesis of chlorophyll c in a dinoflagellate and heterologous production in planta",
abstract = "Chlorophyll c is a key photosynthetic pigment that has been used historically to classify eukaryotic algae. Despite its importance in global photosynthetic productivity, the pathway for its biosynthesis has remained elusive. Here we define the CHLOROPHYLL C SYNTHASE (CHLCS) discovered through investigation of a dinoflagellate mutant deficient in chlorophyll c. CHLCSs are proteins with chlorophyll a/b binding and 2-oxoglutarate-Fe(II) dioxygenase (2OGD) domains found in peridinin-containing dinoflagellates; other chlorophyll c-containing algae utilize enzymes with only the 2OGD domain or an unknown synthase to produce chlorophyll c. 2OGD-containing synthases across dinoflagellate, diatom, cryptophyte, and haptophyte lineages form a monophyletic group, 8 members of which were also shown to produce chlorophyll c. Chlorophyll c1 to c2 ratios in marine algae are dictated in part by chlorophyll c synthases. CHLCS heterologously expressed in planta results in the accumulation of chlorophyll c1 and c2, demonstrating a path to augment plant pigment composition with algal counterparts.",
keywords = "2 oxoglutarate-Fe(II) dioxygenase, biosynthesis, Breviolum minutum, CHLCS, chlorophyll c, CHLOROPHYLL C SYNTHASE, chromalveolate, dinoflagellate",
author = "Jinkerson, {Robert E.} and Daniel Poveda-Huertes and Cooney, {Elizabeth C.} and Anna Cho and Rocio Ochoa-Fernandez and Keeling, {Patrick J.} and Tingting Xiang and Johan Andersen-Ranberg",
note = "Publisher Copyright: {\textcopyright} 2023 The Author(s)",
year = "2024",
doi = "10.1016/j.cub.2023.12.068",
language = "English",
volume = "34",
pages = "594--605.e4",
journal = "Current Biology",
issn = "0960-9822",
publisher = "Cell Press",
number = "3",

}

RIS

TY - JOUR

T1 - Biosynthesis of chlorophyll c in a dinoflagellate and heterologous production in planta

AU - Jinkerson, Robert E.

AU - Poveda-Huertes, Daniel

AU - Cooney, Elizabeth C.

AU - Cho, Anna

AU - Ochoa-Fernandez, Rocio

AU - Keeling, Patrick J.

AU - Xiang, Tingting

AU - Andersen-Ranberg, Johan

N1 - Publisher Copyright: © 2023 The Author(s)

PY - 2024

Y1 - 2024

N2 - Chlorophyll c is a key photosynthetic pigment that has been used historically to classify eukaryotic algae. Despite its importance in global photosynthetic productivity, the pathway for its biosynthesis has remained elusive. Here we define the CHLOROPHYLL C SYNTHASE (CHLCS) discovered through investigation of a dinoflagellate mutant deficient in chlorophyll c. CHLCSs are proteins with chlorophyll a/b binding and 2-oxoglutarate-Fe(II) dioxygenase (2OGD) domains found in peridinin-containing dinoflagellates; other chlorophyll c-containing algae utilize enzymes with only the 2OGD domain or an unknown synthase to produce chlorophyll c. 2OGD-containing synthases across dinoflagellate, diatom, cryptophyte, and haptophyte lineages form a monophyletic group, 8 members of which were also shown to produce chlorophyll c. Chlorophyll c1 to c2 ratios in marine algae are dictated in part by chlorophyll c synthases. CHLCS heterologously expressed in planta results in the accumulation of chlorophyll c1 and c2, demonstrating a path to augment plant pigment composition with algal counterparts.

AB - Chlorophyll c is a key photosynthetic pigment that has been used historically to classify eukaryotic algae. Despite its importance in global photosynthetic productivity, the pathway for its biosynthesis has remained elusive. Here we define the CHLOROPHYLL C SYNTHASE (CHLCS) discovered through investigation of a dinoflagellate mutant deficient in chlorophyll c. CHLCSs are proteins with chlorophyll a/b binding and 2-oxoglutarate-Fe(II) dioxygenase (2OGD) domains found in peridinin-containing dinoflagellates; other chlorophyll c-containing algae utilize enzymes with only the 2OGD domain or an unknown synthase to produce chlorophyll c. 2OGD-containing synthases across dinoflagellate, diatom, cryptophyte, and haptophyte lineages form a monophyletic group, 8 members of which were also shown to produce chlorophyll c. Chlorophyll c1 to c2 ratios in marine algae are dictated in part by chlorophyll c synthases. CHLCS heterologously expressed in planta results in the accumulation of chlorophyll c1 and c2, demonstrating a path to augment plant pigment composition with algal counterparts.

KW - 2 oxoglutarate-Fe(II) dioxygenase

KW - biosynthesis

KW - Breviolum minutum

KW - CHLCS

KW - chlorophyll c

KW - CHLOROPHYLL C SYNTHASE

KW - chromalveolate

KW - dinoflagellate

U2 - 10.1016/j.cub.2023.12.068

DO - 10.1016/j.cub.2023.12.068

M3 - Journal article

C2 - 38157859

AN - SCOPUS:85181254635

VL - 34

SP - 594-605.e4

JO - Current Biology

JF - Current Biology

SN - 0960-9822

IS - 3

ER -

ID: 383748301